Zobrazeno 1 - 10
of 18
pro vyhledávání: '"Robert Kinach"'
Publikováno v:
Biomacromolecules. 12:3997-4010
We describe the synthesis of metal-chelating polymers (MCPs) with four different pendant polyaminocarboxylate ligands (EDTA, DTPA, TTHA, DOTA) and an orthogonal end-group, either a fluorescein molecule or a bismaleimide linker for antibody attachment
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f92085c89060cf6cb14bc4d7476d1ee
https://doi.org/10.1021/bm201011t
https://doi.org/10.1021/bm201011t
Autor:
Dmitry Bandura, Scott D. Tanner, Sergey Vorobiev, Olga Ornatsky, Serguei Pavlov, Xudong Lou, Robert Kinach, Alexei Antonov, Vladimir I. Baranov, John E. Dick
Publikováno v:
Analytical Chemistry. 81:6813-6822
A novel instrument for real time analysis of individual biological cells or other microparticles is described. The instrument is based on inductively coupled plasma time-of-flight mass spectrometry and comprises a three-aperture plasma-vacuum interfa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20bb89ceae045f29edbdba617d10509d
https://doi.org/10.1021/ac901049w
https://doi.org/10.1021/ac901049w
Autor:
Olga Ornatsky, Robert Kinach, Vladimir Baranov, Isaac Herrera, Guohua Zhang, Mark Nitz, Mitchell A. Winnik, Xudong Lou
Publikováno v:
Angewandte Chemie. 119:6223-6226
To identify a rare (e.g., diseased or foreign) cell in a complex mixture, or to understand the proteomic complexity[1,2] of cells, one needs to be able to measure simultaneously and quantitatively a large number of proteins or other biomarkers that m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1926b0e1b224b77e03b9f2a9f55eb352
https://doi.org/10.1002/ange.200700796
https://doi.org/10.1002/ange.200700796
Autor:
Donald W. Hughes, Gerard D. Wright, Suzanne L. Jacques, Hoi Kiong Lai, Albert M. Berghuis, Linda Ejim, John F. Honek, I. Ahmad Mirza, Kirk Green, Kalinka Koteva, Robert Kinach
Publikováno v:
Chemistry & Biology. 10:989-995
The structure of the antifungal drug 5-hydroxy-4-oxonorvaline (HON) in complex with its target homoserine dehydrogenase (HSD) has been determined by X-ray diffraction to 2.6 A resolution. HON shows potent in vitro and in vivo activity against various
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::750741a5fdca5cd6216d48ff9e2ac146
https://doi.org/10.1016/j.chembiol.2003.09.015
https://doi.org/10.1016/j.chembiol.2003.09.015
Autor:
Gerard D. Wright, Suzanne L. Jacques, Catharine Nieman, David C. Bareich, Greg Broadhead, John F. Honek, Robert Kinach
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1544:28-41
Fungal homoserine dehydrogenase (HSD) is required for the biosynthesis of threonine, isoleucine and methionine from aspartic acid, and is a target for antifungal agents. HSD from the yeast Saccharomyces cerevisiae was overproduced in Escherichia coli
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d23784ea2a0f62a1d040f3894a504655
https://doi.org/10.1016/s0167-4838(00)00203-x
https://doi.org/10.1016/s0167-4838(00)00203-x
Autor:
Johanna M. Rommens, Christopher Hicks, Robert Kinach, Danuta Zylka, Delphine Lechardeur, Luke Drzymala, Nawaid Usmani, Manu Sharma, Gergely L. Lukacs, Joanna Pacia
Publikováno v:
The Journal of Cell Biology
Programmed cell death or apoptosis leads to the activation of the caspase-activated DNase (CAD), which degrades chromosomal DNA into nucleosomal fragments. Biochemical studies revealed that CAD forms an inactive heterodimer with the inhibitor of casp
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0cc11d46e23dbfe2155dae740ded1df2
https://doi.org/10.1083/jcb.150.2.321
https://doi.org/10.1083/jcb.150.2.321
Autor:
Robert Kinach, Elisabeth Daub, Robert Ruman, Susan L. Clugston, John F. Barnard, John F. Honek, Denise Miedema
Publikováno v:
Biochemistry. 37:8754-8763
The ubiquitous glyoxalase system converts toxic alpha-keto aldehydes into their corresponding nontoxic 2-hydroxycarboxylic acids, utilizing glutathione (GSH) as a cofactor. The first enzyme in this system, glyoxalase I (GlxI), catalyzes the isomeriza
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d29d65e47d12eff146f7a271cf75c73
https://doi.org/10.1021/bi972791w
https://doi.org/10.1021/bi972791w
Autor:
Susan L. Clugston, John F. Barnard, John F. Honek, Elisabeth Daub, Denise Miedema, Robert Kinach
Publikováno v:
Gene. 186:103-111
The glyoxalase I gene (gloA) from Salmonella typhimurium has been isolated in Escherichia coli on a multi-copy pBR322-derived plasmid, selecting for resistance to 3 mM methylglyoxal on Luria-Bertani agar. The region of the plasmid which confers the m
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b3d10f8e6abe1396c30d5b661aaea92f
https://doi.org/10.1016/s0378-1119(96)00691-9
https://doi.org/10.1016/s0378-1119(96)00691-9
Autor:
Olga Ornatsky, Mark Nitz, Dmitry R. Bandura, Mitchell A. Winnik, Vladimir Baranov, Scott D. Tanner, Xudong Lou, Robert Kinach
Publikováno v:
Journal of analytical atomic spectrometry. 23(4)
Advances in the development of highly multiplexed bio-analytical assays with inductively coupled plasma mass spectrometry (ICP-MS) detection are discussed. Use of novel reagents specifically designed for immunological methods utilizing elemental anal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::012d7b4b23c929612235c6a26f09877b
https://pubmed.ncbi.nlm.nih.gov/19122859
https://pubmed.ncbi.nlm.nih.gov/19122859
Autor:
Olga Ornatsky, Eric R. Lechman, Michael Milyavsky, Scott D. Tanner, Vladimir Baranov, Robert Kinach, Mitchell A. Winnik, Eva J. Razumienko
Publikováno v:
Journal of immunological methods. 336(1)
We have investigated the possibility of using element-tagged antibodies for protein detection and quantification in microplate format using Inductively Coupled Plasma Mass Spectrometry (ICP-MS), and compared the results to conventional immunoassays,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::71ac0e71fcd6f0a3bf77d1af263a0828
https://pubmed.ncbi.nlm.nih.gov/18456275
https://pubmed.ncbi.nlm.nih.gov/18456275