Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Robert J. Pryor"'
Autor:
Zhonglou Sun, Helong Zhao, Daniel Fang, Chadwick T. Davis, Dallas S. Shi, Kachon Lei, Bianca E. Rich, Jacob M. Winter, Li Guo, Lise K. Sorensen, Robert J. Pryor, Nina Zhu, Samuel Lu, Laura L. Dickey, Daniel J. Doty, Zongzhong Tong, Kirk R. Thomas, Alan L. Mueller, Allie H. Grossmann, Baowei Zhang, Thomas E. Lane, Robert S. Fujinami, Shannon J. Odelberg, Weiquan Zhu
Publikováno v:
Neuron. 110:3106-3120.e7
Breakdown of the blood-central nervous system barrier (BCNSB) is a hallmark of many neuroinflammatory disorders, such as multiple sclerosis (MS). Using a mouse model of MS, experimental autoimmune encephalomyelitis (EAE), we show that endothelial-to-
Autor:
Robert J. Pryor, Zachary Dwight, Robert Palais, Scott O. Sundberg, Joseph T. Myrick, Jarkko J Huuskonen, Sean J Ison, Lindsay N. Sanford, Carl T. Wittwer
Publikováno v:
Clinical chemistry. 65(2)
BACKGROUND Extreme PCR in METHODS A microfluidic platform was enhanced for speed using cycle times as fast as 1.05 s between 66.4 °C and 93.7 °C, with end point melting rates of 8 °C/s. Primer and polymerase concentrations were increased to allow
Autor:
Jared S. Farrar, Renée M. Howell, Heather M. Stiles, Robert J. Pryor, Robert Palais, Ivor T. Knight, Jarkko J Huuskonen, Carl T. Wittwer, Scott O. Sundberg
Publikováno v:
Clinical Chemistry. 60:1306-1313
BACKGROUND Clinical molecular testing typically batches samples to minimize costs or uses multiplex lab-on-a-chip disposables to analyze a few targets. In genetics, multiple variants need to be analyzed, and different work flows that rapidly analyze
Autor:
Ivor T. Knight, Robert J. Pryor, Joseph T. Myrick, Carl T. Wittwer, Robert Palais, Scott O. Sundberg, Jeanette Y. Paek
Publikováno v:
Clinical chemistry. 63(10)
BACKGROUND High-resolution DNA melting analysis of small amplicons is a simple and inexpensive technique for genotyping. Microfluidics allows precise and rapid control of temperature during melting. METHODS Using a microfluidic platform for serial PC
Autor:
Carl T. Wittwer, Robert J. Pryor, Harry R. Hill, Anne E. Tebo, Javier Chinen, Jeffrey M. Bender, Joshua D. Bagnato, Brian M. Pasi, I. Celine Hanson, Nancy H. Augustine, Dirk Roos, Martin de Boer, Gudrun H. Reed
Publikováno v:
Journal of molecular diagnostics, 12(3), 368-376. Association of Molecular Pathology
High-resolution melting analysis was applied to X-linked chronic granulomatous disease, a rare disorder resulting from mutations in CYBB. Melting curves of the 13 PCR products bracketing CYBB exons were predicted by Poland's algorithm and compared wi
Autor:
Robert J. Pryor, Nancy H. Augustine, Mark Leppert, John C. Carey, Harry R. Hill, Ralph J. Faville, Hans D. Ochs, Ralph J. Wedgwood, Carl T. Wittwer, Paul G. Quie, Attila Kumánovics
Publikováno v:
The Journal of Molecular Diagnostics. 12:213-219
With the recent discovery of mutations in the STAT3 gene in the majority of patients with classic Hyper-IgE syndrome, it is now possible to make a molecular diagnosis in most of these cases. We have developed a PCR-based high-resolution DNA-melting a
Publikováno v:
Clinical Chemistry. 51:1770-1777
Background: High-resolution DNA melting analysis with saturation dyes for either mutation scanning of PCR products or genotyping with unlabeled probes has been reported. However, simultaneous PCR product scanning and probe genotyping in the same reac
Publikováno v:
Genomics. 39:136-146
The proximal end of mouse chromosome (Chr) 13 contains regions conserved on human chromosomes 1q42-q44, 6p23-p21, and 7p22-p13. This region also contains mutations that may be models for human disease, including beige (human Chediak-Higashi syndrome)
Publikováno v:
Proceedings of the National Academy of Sciences. 93:5905-5909
Chédiak-Higashi syndrome in man and the beige mutation of mice are phenotypically similar disorders that have profound effects upon lysosome and melanosome morphology and function. We isolated two murine yeast artificial chromosomes (YACs) that, whe
Autor:
Robert J. Pryor, Carl T. Wittwer
Monitoring polymerase chain reaction (PCR) once each cycle is a powerful method to detect and quantify the presence of nucleic acid sequences and has become known as "real-time" PCR. Absolute quantification of initial template copy number can be obta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::e1fcc6c7a67b885704b5dd4dc5c471d1
https://doi.org/10.1385/1-59745-074-x:19
https://doi.org/10.1385/1-59745-074-x:19