Výsledky vyhledávání - "Robert J. Ihry"

Akademický článek

Genome-Scale CRISPR Screens Identify Human Pluripotency-Specific Genes

Publikováno v: Cell Reports, Vol 27, Iss 2, Pp 616-630.e6 (2019)

Summary: Human pluripotent stem cells (hPSCs) generate a variety of disease-relevant cells that can be used to improve the translation of preclinical research. Despite the potential of hPSCs, their use for genetic screening has been limited by techni

Externí odkaz: https://doaj.org/article/39ad2f3ea8e94904a721c8785edecb36

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DRUG-seq Provides Unbiased Biological Activity Readouts for Neuroscience Drug Discovery

Publikováno v: ACS chemical biology. 17(6)

Unbiased transcriptomic RNA-seq data has provided deep insights into biological processes. However, its impact in drug discovery has been narrow given high costs and low throughput. Proof-of-concept studies with Digital RNA with pertUrbation of Genes

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::84df92c55bb23f5e3de70243ffd28703
https://pubmed.ncbi.nlm.nih.gov/35508359

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Patient brain organoids identify a link between the 16p11.2 copy number variant and the RBFOX1 gene

Autor: S. H. Cheon, J. Chung, Tayfun Tumkaya, Joe Raymond, Ricardo E. Dolmetsch, Katie A. Worringer, Y. Sun, B. Henry, J. Dvornik, J. Khlghatyan, Robert J. Ihry, M. Kostic, J. Hsiao

SUMMARYCopy number variants (CNVs) that delete or duplicate 30 genes within the 16p11.2 genomic region give rise to a range of neurodevelopmental phenotypes with high penetrance in humans. Despite the identification of this small region, the mechanis

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::653141757fd03785ec9c163905ad5486
https://doi.org/10.1101/2021.11.21.469432

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DRUG-seq Provides Unbiased Biological Activity Readouts for Drug Discovery

Autor: Kathleen A. Worringer, Robert J. Ihry, Marc Hild, Carsten Russ, Marilisa Neri, Leandra Mansur, Alicia Lindeman, Frederic Sigoillot, Chian Yang, Robin Ge, Jeremy L. Jenkins, Daniel J. Ho, Caroline Gubser Keller, Tayfun Tumkaya, Jingyao Li, Steffen Renner, Martin Henault

Unbiased transcriptomic RNA-seq data has provided deep insights about biological processes. However, its impact in drug discovery has been narrow given high costs and low throughput. Proof-of-concept studies with Digital RNA with pertUrbation of Gene

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c4d7c984cbca815756ba3415d0008b95
https://doi.org/10.1101/2021.06.07.447456

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Akademický článek

Translational control by the DEAD Box RNA helicase belle regulates ecdysone-triggered transcriptional cascades.

Autor: Robert J Ihry, Anne L Sapiro, Arash Bashirullah

Publikováno v: PLoS Genetics, Vol 8, Iss 11, p e1003085 (2012)

Steroid hormones act, through their respective nuclear receptors, to regulate target gene expression. Despite their critical role in development, physiology, and disease, however, it is still unclear how these systemic cues are refined into tissue-sp

Externí odkaz: https://doaj.org/article/0fcfc4f0d80a4b458c3bb3ee08b4d99d

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DRUG-seq for miniaturized high-throughput transcriptome profiling in drug discovery

Autor: Robert J. Ihry, Nadezda Mostacci, Gregory McAllister, Martin Henault, Caroline Gubser Keller, Jeremy L. Jenkins, Steffen Renner, Daniel J. Ho, Chaoyang Ye, Ajamete Kaykas, Marilisa Neri, Chian Yang, Leandra Mansur, Marc Hild, Tripti Kulkarni, Pierre Farmer, Ranjit Randhawa

Publikováno v: Nature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
Nature Communications

Here we report Digital RNA with pertUrbation of Genes (DRUG-seq), a high-throughput platform for drug discovery. Pharmaceutical discovery relies on high-throughput screening, yet current platforms have limited readouts. RNA-seq is a powerful tool to

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d9089b74819c1f0e0dfaf422f38c70de
https://doi.org/10.1038/s41467-018-06500-x

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Genome-Scale CRISPR Screening Identifies Novel Human Pluripotent Gene Networks

Autor: Gregory R. Hoffman, Kathleen A. Worringer, Ranjit Randhawa, Sravya Kommineni, Robert J. Ihry, Gregory McAllister, Marie Sondey, Steven Paula, Ricardo E. Dolmetsch, Zinger Yang, Ajamete Kaykas, Elizabeth Frias, Daniel J. Ho, Joe Raymond, Bob Altshuler, Max R. Salick, Carsten Russ, John S. Reece-Hoyes

Publikováno v: SSRN Electronic Journal.

Human pluripotent stem cells (hPSCs) generate a wide variety of disease-relevant cells that can be used to improve the translation of preclinical research. Despite the potential of hPSCs, their use for genetic screening has been limited because of te

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0c345a1bdb5c383f6a79f625d6ac07ed
https://doi.org/10.2139/ssrn.3189387

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p53 inhibits CRISPR-Cas9 engineering in human pluripotent stem cells

Publikováno v: Nature medicine. 24(7)

CRISPR/Cas9 has revolutionized our ability to engineer genomes and conduct genome-wide screens in human cells1–3. Whereas some cell types are amenable to genome engineering, genomes of human pluripotent stem cells (hPSCs) have been difficult to eng

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7f9a2862fe1c264657cc670822d146f
https://pubmed.ncbi.nlm.nih.gov/30017531

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Genetic Control of Specificity to Steroid-Triggered Responses in Drosophila

Autor: Arash Bashirullah, Robert J. Ihry

Publikováno v: Genetics. 196:767-780

Steroid hormones trigger a wide variety of biological responses through stage- and tissue-specific activation of target gene expression. The mechanisms that provide specificity to systemically released pulses of steroids, however, remain poorly under

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9f7605654b5ab7f51fbeafa67e2d324
https://doi.org/10.1534/genetics.113.159707

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