Zobrazeno 1 - 7
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pro vyhledávání: '"Robert Hooftman"'
Autor:
Daniel G. Olson, Shuen Hon, Jeroen Girwar Koendjbiharie, Richard van Kranenburg, David M. Stevenson, Daniel Amador-Noguez, Martin Pabst, Jingxuan Cui, Lee R. Lynd, Robert Hooftman
Publikováno v:
Journal of Biological Chemistry, 295(7), 1867-1878
Journal of Biological Chemistry 295 (2020) 7
The Journal of Biological Chemistry
Journal of Biological Chemistry, 295(7)
Journal of Biological Chemistry 295 (2020) 7
The Journal of Biological Chemistry
Journal of Biological Chemistry, 295(7)
The genomes of most cellulolytic clostridia do not contain genes annotated as transaldolase. Therefore, for assimilating pentose sugars or for generating C5 precursors (such as ribose) during growth on other (non-C5) substrates, they must possess a p
Autor:
Robert Hooftman
This protocol can be used to measure the binding capacity of chitin binding proteins and visualizing this using a Bradford assay. The protocol has been adapted from: F. Labroussaa, A. R. Zeilinger, and R. P. P. Almeida, “Blocking the Transmission o
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::99a36b0f64920175ebe7ce7331612713
https://doi.org/10.17504/protocols.io.8akhscw
https://doi.org/10.17504/protocols.io.8akhscw
Autor:
Robert Hooftman
In this protocol, the preparation and running an acrylamide gel is explained. Using this gel, proteins with a size of >15 kDa can be visualized. For the visualization of proteins with a size of 15 -30 kDa, a 12% acrylamide gel can be used. For the vi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::95a31f653384bef2c88f37835bc05317
https://doi.org/10.17504/protocols.io.7syhnfw
https://doi.org/10.17504/protocols.io.7syhnfw
Autor:
Robert Hooftman
In this protocol, the preparation and running of a tricine-urea gel will be explained.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7651495eba7cdab582d1b93118bb3f1e
https://doi.org/10.17504/protocols.io.7sxhnfn
https://doi.org/10.17504/protocols.io.7sxhnfn
Autor:
Robert Hooftman
In this protocol, the preparation and running an acrylamide gel is explained. Using this gel, proteins with a size of >15 kDa can be visualized. For the visualization of proteins with a size of 15 -30 kDa, a 12% acrylamide gel can be used. For the vi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::004472c6610d1867fdfc23d0b2633e22
https://doi.org/10.17504/protocols.io.7q9hmz6
https://doi.org/10.17504/protocols.io.7q9hmz6
Autor:
Robert Hooftman
In this protocol will be explained how to express proteins using E. coli strain BL21DE3 using a plasmid with a T7 promoter. The promoter of T7 polymerase from E. coli BL21DE3 has to be induced with IPTG.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::70859a01167ba9da51955e6a1aeaf3ee
https://doi.org/10.17504/protocols.io.7i4hkgw
https://doi.org/10.17504/protocols.io.7i4hkgw