Zobrazeno 1 - 10
of 59
pro vyhledávání: '"Robert H. Rownd"'
Publikováno v:
Journal of Bacteriology. 173:2378-2384
The stability (stb) locus of IncFII plasmid NR1 is composed of an essential cis-acting DNA site located upstream from two tandem genes that encode essential stability proteins. The stb locus was found to be transcribed from a promoter site just upstr
Publikováno v:
Proceedings of the National Academy of Sciences. 71:2188-2192
In vivo , ribosomal RNA of Saccharomyces cerevisiae is transcribed from the light strand of γ DNA. In vitro , γ DNA is transcribed with equal efficiency by both of the yeast nuclear RNA polymerases, polymerase I and polymerase II; however the RNA p
Autor:
David D. Womble, Robert H. Rownd
Publikováno v:
Journal of Molecular Biology. 191:367-382
A quantitative model for the regulation of replication of the low copy number IncFII plasmid NR1 in the Escherichia coli cell division cycle has been developed. The initiation of NR1 replication requires a cis-acting initiator protein whose synthesis
Autor:
Daniel Perlman, Robert H. Rownd
Publikováno v:
Nature. 259:281-284
Electron microscopic examination of a composite R plasmid made up of an RTF component and an r-determinants component shows that replication may originate from either the RTF origin or the r-determinants origin of replication.
Accumulation of replicating bacterial plasmid DNA during thymine limitation or hydroxyurea treatment
Autor:
Dan Perlman, Robert H. Rownd
Publikováno v:
Molecular and General Genetics MGG. 138:281-291
The rate of DNA chain elongation of a thymine auxotroph of Proteus mirabilis harboring an R factor was reduced by either thymine limitation or treatment with hydroxyurea. Using CsCl density gradient centrifugation, sedimentation in sucrose gradients,
Publikováno v:
Molecular and General Genetics MGG. 148:233-241
The size and degree of homogeneity of the repetitive units in purified ribosomal DNA (gamma DNA) from Saccharomyces cerevisiae have been analyzed by restriction endonuclease digestion and heteroduplex mapping. Digestion of the gamma DNA with EcoRI yi
Publikováno v:
Journal of Bacteriology. 123:1035-1042
The R factor NR1 consists of two components: a resistance transfer factor which harbors the tetracycline resistance genes (RTF-TC) and the r-determinants component which harbors the other drug resistance genes. Using partial denaturation mapping it i
Autor:
Robert H. Rownd, Jane Harris Cramer
Publikováno v:
Molecular and General Genetics MGG. 177:199-205
The thermal melting profile of purified Saccharomyces cerevisiae ribosomal DNA (rDNA) is biphasic indicating considerable intramolecular heterogeneity in base composition. The first phase of the transition, about 20% of the total hyperchromic shift,
Autor:
David D. Womble, Robert H. Rownd
Publikováno v:
Journal of Molecular Biology. 192:529-547
Publikováno v:
FEBS Letters. 143:129-132
In most eukaryotic cells, the genes for the ribosomal RNAs (rRNAs) occur as tandemly repeated units arranged in head-to-tail arrays within the chromosome. The transcription unit for the 18 S, 5.8 S and 25 S rRNAs alternates with a non-transcribed ‘