Zobrazeno 1 - 10
of 434
pro vyhledávání: '"Robert H, Singer"'
Autor:
Valentina Gandin, Brian P. English, Melanie Freeman, Louis-Philippe Leroux, Stephan Preibisch, Deepika Walpita, Maritza Jaramillo, Robert H. Singer
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
mRNA translation is tightly regulated to preserve cellular homeostasis. Here live-cell spectroscopy and single-particle tracking are combined to interrogate the binding dynamics of endogenous initiation factors to mRNAs 5’cap, revealing the sequenc
Externí odkaz:
https://doaj.org/article/1dfaaf506e714133b4cf6ed43d9eef28
Autor:
Rajiv Pathak, Carolina Eliscovich, Ignacio Mena, Anastasija Cupic, Magdalena Rutkowska, Kartik Chandran, Rohit K. Jangra, Adolfo García-Sastre, Robert H. Singer, Ganjam V. Kalpana
Publikováno v:
Viruses, Vol 16, Iss 2, p 262 (2024)
SARS-CoV-2 infection remains a global burden. Despite intensive research, the mechanism and dynamics of early viral replication are not completely understood, such as the kinetics of the formation of genomic RNA (gRNA), sub-genomic RNA (sgRNA), and r
Externí odkaz:
https://doaj.org/article/5eb3557e958b465e866222b03412dab6
Autor:
Marc Corrales, Benjamin T. Cocanougher, Andrea B. Kohn, Jason D. Wittenbach, Xi S. Long, Andrew Lemire, Albert Cardona, Robert H. Singer, Leonid L. Moroz, Marta Zlatic
Publikováno v:
Neural Development, Vol 17, Iss 1, Pp 1-23 (2022)
Abstract Molecular profiles of neurons influence neural development and function but bridging the gap between genes, circuits, and behavior has been very difficult. Here we used single cell RNAseq to generate a complete gene expression atlas of the D
Externí odkaz:
https://doaj.org/article/8da66f866b114055b6916e18666e7aca
Autor:
Sandipan Dasgupta, Daniella Y Dayagi, Gal Haimovich, Emanuel Wyler, Tsviya Olender, Robert H Singer, Markus Landthaler, Jeffrey E Gerst
Publikováno v:
eLife, Vol 12 (2023)
Full-length mRNAs transfer between adjacent mammalian cells via direct cell-to-cell connections called tunneling nanotubes (TNTs). However, the extent of mRNA transfer at the transcriptome-wide level (the ‘transferome’) is unknown. Here, we analy
Externí odkaz:
https://doaj.org/article/92dc7283d54146dead5e2628d03760f7
Autor:
Hotaka Kobayashi, Robert H. Singer
Publikováno v:
Nature Communications, Vol 13, Iss 1, Pp 1-14 (2022)
For decades, miRNAs have been studied primarily by ensemble methods, where a bulk collection of molecules is measured outside cells. Here, Kobayashi and Singer report methods to image miRNA function at the single-molecule level inside cells.
Externí odkaz:
https://doaj.org/article/a1598bc3a716448ba5aad096c5910c38
Autor:
Hanae Sato, Robert H. Singer
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Here the author developed a single-cell reporter system to identify cell-to-cell variability of nonsense-mediated mRNA decay (NMD). This approach provides a sensitive tool to investigate cellular heterogeneity of NMD in various physiological conditio
Externí odkaz:
https://doaj.org/article/18c8478506f94941be7185fd56584eb2
Autor:
Yongmei Feng, Stefan Grotegut, Predrag Jovanovic, Valentina Gandin, Steven H. Olson, Rabi Murad, Anne Beall, Sharon Colayco, Paul De-Jesus, Sumit Chanda, Brian P. English, Robert H. Singer, Michael Jackson, Ivan Topisirovic, Ze’ev A. Ronai
Publikováno v:
Frontiers in Pharmacology, Vol 13 (2022)
The translation initiation complex 4F (eIF4F) is a rate-limiting factor in protein synthesis. Alterations in eIF4F activity are linked to several diseases, including cancer and infectious diseases. To this end, coronaviruses require eIF4F complex act
Externí odkaz:
https://doaj.org/article/3f5893fb1bab4b94b39dab0daec38624
Publikováno v:
Communications Biology, Vol 4, Iss 1, Pp 1-12 (2021)
Liou et al. report a 4.6 Å resolution structure of the human p53/ RNA polymerase II assembly, using single particle cryoelectron microscopy. This study suggests that p53’s functional domains regulate the DNA binding activity of RNA polymerase II,
Externí odkaz:
https://doaj.org/article/be1a87333b6a4982b660b7ba76442e68
Autor:
Marc Corrales, Benjamin T. Cocanougher, Andrea B. Kohn, Jason D. Wittenbach, Xi S. Long, Andrew Lemire, Albert Cardona, Robert H. Singer, Leonid L. Moroz, Marta Zlatic
Publikováno v:
Neural Development, Vol 17, Iss 1, Pp 1-2 (2022)
Externí odkaz:
https://doaj.org/article/01c5924208d64ba2b8919c3a0c4df678
Publikováno v:
STAR Protocols, Vol 2, Iss 3, Pp 100634- (2021)
Summary: Targets of RNA-binding proteins discovered by editing (TRIBE) determines RNA-proteins interactions and nuclear organization with minimal false positives. We detail necessary steps for performing mammalian cell RBP-TRIBE to determine the targ
Externí odkaz:
https://doaj.org/article/69767ced513e4f8a96078c6dc0a9086c