Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Robert G. Blazej"'
Publikováno v:
BioProcessing Journal. 11:45-48
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::a5f2dfb7b14a52dc273a86d6693d03ee
https://doi.org/10.12665/j111.neb
https://doi.org/10.12665/j111.neb
Publikováno v:
Analytical Chemistry. 79:4499-4506
A new affinity-capture-based inline purification, concentration, and injection method is developed for microchip capillary electrophoresis (CE) and used to perform efficient attomole-scale Sanger DNA sequencing separations. The microdevice comprises
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::63964ad4846f9fbe38e4d420ac8dae22
https://doi.org/10.1021/ac070126f
https://doi.org/10.1021/ac070126f
Publikováno v:
Proceedings of the National Academy of Sciences. 103:7240-7245
An efficient, nanoliter-scale microfabricated bioprocessor integrating all three Sanger sequencing steps, thermal cycling, sample purification, and capillary electrophoresis, has been developed and evaluated. Hybrid glass–polydimethylsiloxane (PDMS
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::79dbab07b57a5a4687508bbeba4fc43c
https://doi.org/10.1073/pnas.0602476103
https://doi.org/10.1073/pnas.0602476103
Autor:
Eric T. Lagally, James R. Scherer, Richard A. Mathies, Binh An Diep, Lee W. Riley, George F. Sensabaugh, Robert G. Blazej, Nicholas Toriello, M Ramchandani
Publikováno v:
Analytical Chemistry. 76:3162-3170
An integrated portable genetic analysis microsystem including PCR amplification and capillary electrophoretic (CE) analysis coupled with a compact instrument for electrical control and laser-excited fluorescence detection has been developed. The micr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::628a509a77351b3b7ec8971c93893db4
https://doi.org/10.1021/ac035310p
https://doi.org/10.1021/ac035310p
Publikováno v:
Current Opinion in Biotechnology. 14:42-50
Modern DNA sequencing 'factories' have revolutionized biology by completing the human genome sequence, but in the race to completion we are left with inefficient, cumbersome, and costly macroscale processes and supporting facilities. During the same
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::20cecb251a06b0c6fa4330cafc3230a5
https://doi.org/10.1016/s0958-1669(02)00004-6
https://doi.org/10.1016/s0958-1669(02)00004-6
Autor:
Charles A. Emrich, Igor L. Medintz, James R. Scherer, Robert G. Blazej, Brian M. Paegel, Richard A. Mathies, Lorenzo Berti
Publikováno v:
ELECTROPHORESIS. 22:3845-3856
This review focuses on some recent advances in realizing microfabricated capillary array electrophoresis (microCAE). In particular, the development of a novel rotary scanning confocal fluorescence detector has facilitated the high-speed collection of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c5438032993cf9aa15ce87e4c8c46d9e
https://doi.org/10.1002/1522-2683(200110)22:18<3845::aid-elps3845>3.0.co
https://doi.org/10.1002/1522-2683(200110)22:18<3845::aid-elps3845>3.0.co
Autor:
Samantha A. Cronier, Robert G. Blazej, Palani Kumaresan, Richard A. Mathies, Chaoyong James Yang
Publikováno v:
Analytical chemistry. 80(10)
A high-throughput single copy genetic amplification (SCGA) process is developed that utilizes a microfabricated droplet generator (microDG) to rapidly encapsulate individual DNA molecules or cells together with primer functionalized microbeads in uni
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4cfa2d4b9c3311690ffae497c3f854ae
https://pubmed.ncbi.nlm.nih.gov/18410131
https://pubmed.ncbi.nlm.nih.gov/18410131
Publikováno v:
Analytical chemistry. 79(22)
A novel injection method is developed that utilizes a thermally switchable oligonucleotide affinity capture gel to mediate the concentration, purification, and injection of dsDNA for quantitative microchip capillary electrophoresis analysis. The affi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43e33faa3d44ad78c791e49cdad0236c
https://pubmed.ncbi.nlm.nih.gov/17929900
https://pubmed.ncbi.nlm.nih.gov/17929900
Publikováno v:
Genome research. 13(2)
Polymorphism ratio sequencing (PRS) combines the advantages of high-throughput DNA sequencing with new labeling and pooling schemes to produce a powerful assay for sensitive single nucleotide polymorphism (SNP) discovery, rapid genotyping, and accura
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f32188354add03c6da059a6a8c3602ab
https://pubmed.ncbi.nlm.nih.gov/12566407
https://pubmed.ncbi.nlm.nih.gov/12566407
Publisher Summary This chapter outlines the progress toward novel microfabricated capillary array electrophoresis (μCAE)) devices capable of fully integrated, high-throughput genotyping and DNA sequencing. These integrated bio processors lead the wa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::f682f02f9d06be6442367e154ddb313f
https://doi.org/10.1016/b978-044451100-3/50012-7
https://doi.org/10.1016/b978-044451100-3/50012-7
