Zobrazeno 1 - 10
of 99
pro vyhledávání: '"Robert Eisenthal"'
Publikováno v:
Advances in Polymer Technology. 27:27-34
A temperature-sensitive hydrogel based on a copolymer of BSA and poly(N-isopropylacrylamide) (PNIPAAm) has been synthesized using carbodiimide chemistry. Fourier transform infrared spectroscopy confirmed primary complex formation between carbodiimide
Publikováno v:
Biochemical Society Transactions. 35:1543-1546
Arising from careful measurements of the thermal behaviour of enzymes, a new model, the Equilibrium Model, has been developed to explain more fully the effects of temperature on enzymes. The model describes the effect of temperature on enzyme activit
Publikováno v:
Extremophiles. 12:51-59
The two established thermal properties of enzymes are their activation energy and their thermal stability. Arising from careful measurements of the thermal behaviour of enzymes, a new model, the Equilibrium Model, has been developed to explain more f
Autor:
Robert Eisenthal, S. Craig Cary, David J. Saul, Michael J. Danson, Charis Shepherd, Roy M. Daniel, Michelle E. Peterson, Charles Kai-Wu Lee
Publikováno v:
The FASEB Journal. 21:1934-1941
The "Equilibrium Model" has provided new tools for describing and investigating enzyme thermal adaptation. It has been shown that the effect of temperature on enzyme activity is not only governed by deltaG(double dagger)(cat) and deltaG(double dagger
Publikováno v:
Trends in Biotechnology. 24:289-292
The way that enzymes respond to temperature is fundamental to many areas of biotechnology. This has long been explained in terms of enzyme stability and catalytic activation energy, but recent observations of enzyme behaviour suggest that this pictur
Publikováno v:
Reactive and Functional Polymers. 66:757-767
A facile method is described for the preparation of d -glucose-sensitive hydrogel membranes based on cross-linking carboxymethyl dextran (CM-dextran) with the glucose binding lectin concanavalin A (ConA) using carbodiimide chemistry. The mesh size of
Publikováno v:
Biomaterials. 26:4677-4683
A fast and simple method for the preparation of pH-sensitive hydrogel membranes for drug delivery and tissue engineering applications has been developed using carbodiimide chemistry. The hydrogels were formed by the intermolecular cross-linking of ca
Publikováno v:
Journal of Biological Chemistry. 279:20717-20722
Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activi
Publikováno v:
Biotechnology and Bioengineering. 87:791-796
A hydrogel membrane containing immobilized ligands and receptors was synthesized and investigated for the controlled diffusion of test proteins (cytochrome C and hemoglobin). Both Cibacron blue (ligand) and lysozyme (receptor) were covalently linked
Publikováno v:
Biotechnology and Bioengineering. 82:47-53
Glucose-sensitive hydrogel membranes have been synthesized and characterized for their rate-of-delivery of macromolecules. The mechanism for changing this rate is based on variable displacement of the affinity interaction between dextran and concanav