Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Robert E. Shell"'
Autor:
Sa V. Ho, Kevin J. Rust, Alan K. Hunter, John T. Herberg, Joseph K. McLaughlin, Robert E. Shell, Kristen E. Thomas, Paul B. Lanter, Robert L. Dufield, Bruce F. Bishop, Maire H. Caparon, Xing Wang
Publikováno v:
Biotechnology and Bioengineering. 105:239-249
Apolipoprotein A 1 Milano (ApoA-1M), the protein component of a high-density lipoprotein (HDL) mimic with promising potential for reduction of atherosclerotic plaque, is produced at large scale by expression in E. coli. Significant difficulty with cl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4c1cdbafda045dc7be30d1cf8bf57c81
https://doi.org/10.1002/bit.22542
https://doi.org/10.1002/bit.22542
Autor:
Tze-Chein Wun, Kuniko K Kretzmer, Mark O Palmier, Kathleen C Day, Margaret D Huang, Dean J Welsch, Charles Lewis, Richard A Wolfe, James F Zobel, Gary W Lange, Ronald B Frazier, Gary S Bild, Michelle A Peel, Robert E Shell, Nancy A Horn, Kurt D Junger, Barbara A Foy, Mark E Gustafson, Richard M Leimgruber, William F Novotny, George J Broze, Yvonne E Pyla, Paul J Hippenmeyer, Thomas G Warren
Publikováno v:
Thrombosis and Haemostasis. 68:054-059
SummaryRecombinant tissue factor pathway inhibitor (rTFPI) has been expressed in four mammalian expression systems using human SK hepatoma, mouse C127, baby hamster kidney (BHK), and Chinese hamster ovary (CHO) cells as hosts. On sodium dodecyl sulfa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1d63389b2c57bc358d4345af1819cf2f
https://doi.org/10.1055/s-0038-1656317
https://doi.org/10.1055/s-0038-1656317
Autor:
Eric J. Suda, Sa V. Ho, Robert L. Dufield, Xing Wang, Alan K. Hunter, Robert E. Shell, Mark E. Gustafson, Ned M. Mozier, John T. Herberg, Thomas Kristin Elizabeth
Publikováno v:
Biotechnology progress. 25(2)
We have shown how product associating E. coli host cell proteins (HCPs) OppA and DppA can be substantially separated from apolipoprotein A-I(Milano) (apo A-I(M)) using Butyl Sepharose hydrophobic interaction chromatography (HIC). This work illustrate
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::091ab56ebf84da55a45b5b5e1d2cbabc
https://pubmed.ncbi.nlm.nih.gov/19291803
https://pubmed.ncbi.nlm.nih.gov/19291803
Autor:
Alan K. Hunter, Sa V. Ho, Robert E. Shell, Eric J. Suda, John T. Herberg, Mark E. Gustafson, Thomas Kristin Elizabeth
Publikováno v:
Journal of chromatography. A. 1204(1)
We have shown how protein self-association impacts the ion-exchange separation of modified forms and aggregates for apolipoprotein A-I(Milano). It is well known that reversible self-association of a protein can lead to chromatographic band broadening
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22eb02df86f8bce2acaa8857d6fec897
https://pubmed.ncbi.nlm.nih.gov/18701112
https://pubmed.ncbi.nlm.nih.gov/18701112
Autor:
Tapan K. Das, Mark E. Gustafson, Alan K. Hunter, Eric J. Suda, Robert E. Shell, John T. Herberg, Natraj Ramasubramanyan, Sa V. Ho
Publikováno v:
Biotechnology journal. 2(1)
We have studied the equilibrium uptake behavior and mass transfer rate of recombinant apolipoprotein A-I(Milano) (apo A-I(M)) on Q Sepharose HP under non-denaturing, partially denaturing, and fully denaturing conditions. The protein of interest in th
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::76792879f4409d3e54db3416d48a33a9
https://pubmed.ncbi.nlm.nih.gov/17136731
https://pubmed.ncbi.nlm.nih.gov/17136731
Autor:
Amit Banerjee, Denis M. Boyle, Gary V. Johnson, Robert E. Shell, Mark E. Gustafson, Robert A. Heeren
Publikováno v:
Biotechnology and Applied Biochemistry. 49:73
Conditions to obtain correctly folded PMP-1a (promegapoietin-1a), an engineered fusion IL-3 (interleukin-3) and thrombopoietin receptor agonist from recombinant Escherichia coli IBs (inclusion bodies), were defined to generate sufficient amounts of p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::06ab63385bd81ba8b339c28cbbe159a4
https://doi.org/10.1042/ba20070069
https://doi.org/10.1042/ba20070069
Autor:
Denis M. Boyle, Gary V. Johnson, Robert A. Heeren, Robert E. Shell, Amit Banerjee, Mark E. Gustafson
Publikováno v:
Biotechnology & Applied Biochemistry; 2008, Vol. 049 Issue 1, p73-83, 11p