Zobrazeno 1 - 10
of 26
pro vyhledávání: '"Robert E. Dale"'
Autor:
Melissa K. Corbett, Simon Ameer-Beg, James Hunt, Rebecca L. Beavil, Marcus J. Swann, Robert E. Dale, Brian J. Sutton, Klaus Suhling, Andrew J. Beavil, James A. Levitt, Anthony H. Keeble
Publikováno v:
Journal of Biological Chemistry. 287:17459-17470
IgE binding to its high affinity receptor FcϵRI on mast cells and basophils is a key step in the mechanism of allergic disease and a target for therapeutic intervention. Early indications that IgE adopts a bent structure in solution have been confir
Publikováno v:
Chemical Physics Letters. 438:113-118
A deterministic identifiability analysis of kinetic models of a reversible intramolecular excited-state process involving species-dependent rotational Brownian diffusion is performed for spherical and cylindrically symmetric rotors. It is assumed tha
Autor:
Susan Criddle, Birgit Brandmeier, Andrew S. Brack, Robert E. Dale, Roisean E. Ferguson, Malcolm Irving
Publikováno v:
Biophysical Journal. 86:2329-2341
The orientation of the regulatory light chain (RLC) region of the myosin heads in relaxed skinned fibers from rabbit psoas muscle was investigated by polarized fluorescence from bifunctional rhodamine (BR) probes cross-linking pairs of cysteine resid
Publikováno v:
Biophysical Journal. 83(2):1050-1073
The method of polarized fluorescence depletion (PFD) has been applied to enhance the resolution of orientational distributions and dynamics obtained from fluorescence polarization (FP) experiments on ordered systems, particularly in muscle fibers. Pr
Autor:
John Kendrick-Jones, S. Criddle, Birgit Brandmeier, David R. Trentham, Seth C. Hopkins, Cibele Sabido-David, Malcolm Irving, Yale E. Goldman, U. A. van der Heide, John E. T. Corrie, Roisean E. Ferguson, Robert E. Dale
Publikováno v:
Nature. 400:425-430
A new method is described for measuring motions of protein domains in their native environment on the physiological timescale. Pairs of cysteines are introduced into the domain at sites chosen from its static structure and are crosslinked by a bifunc
Publikováno v:
Biophysical Journal. 76:1606-1618
The orientation of proteins in ordered biological samples can be investigated using steady-state polarized fluorescence from probes conjugated to the protein. A general limitation of this approach is that the probes typically exhibit rapid orientatio
Publikováno v:
Journal of the American Chemical Society. 130(50)
Replica exchange molecular dynamics (REMD) calculations were used to determine the conformation and dynamics of bifunctional rhodamine probes attached to pairs of cysteines in three model systems: (a) a polyalanine helix, (b) the isolated C helix (re
Autor:
Andrea C. Knowles, Yin-Biao Sun, Birgit Brandmeier, John E. T. Corrie, Malcolm Irving, Brian D. Sykes, Robert E. Dale, David R. Trentham, Olivier Julien
Publikováno v:
Biophysical journal. 93(3)
As part of a program to develop methods for determining protein structure in situ, sTnC was labeled with a bifunctional rhodamine (BR or BSR), cross-linking residues 56 and 63 of its C-helix. NMR spectroscopy of the N-terminal domain of BSR-labeled s
A model for analyzing Forster resonance energy transfer (FRET) data in relation to the cell plasma membrane raft hypothesis is developed to take into account: (a) the distribution of FRET donors and acceptors at the surface of probing antibody fragme
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e696d3270f919edd7f14e05db164ee46
http://hdl.handle.net/1942/1682
http://hdl.handle.net/1942/1682
Publikováno v:
Biochemistry. 41(41)
The interaction of two bioactive, fluorescent analogues of the anticancer drug Taxol, Flutax1 [7-O-[N-(fluorescein-4'-carbonyl)-L-alanyl]taxol] and Flutax2 [7-O-[N-(2,7-difluorofluorescein-4'-carbonyl)-L-alanyl]taxol], with microtubules in solution h