Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Robert Dupras"'
Autor:
Thierry Vernet, Robert Ménard, David Y. Thomas, Céline Plouffe, Henry Khouri, Pierre Laflamme, Robert Dupras, Daniel C. Tessier, Andrew C. Storer
Publikováno v:
Biochemistry. 30:5531-5538
In a previous study, it was shown that replacing Asp158 in papain by Asn had little effect on activity and that the negatively charged carboxylate of Asp158 does not significantly stabilize the active site thiolate-imidazolium ion pair of papain (Mé
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccd8866ee07b5ab90f9ff5f0d7777555
https://doi.org/10.1021/bi00236a028
https://doi.org/10.1021/bi00236a028
Autor:
Thierry Vernet, Robert Dupras, Robert Ménard, H E Khouri, Andrew C. Storer, Lalberté F, David Y. Thomas, Daniel C. Tessier, Céline Plouffe, Daniel R. Ripoll
Publikováno v:
Biochemistry. 29:6706-6713
The controversy concerning the various suggested roles for the side chain of Asp158 in the active site of papain has been clarified by using site-directed mutagenesis. Both wild-type papain and an Asp158 Asn variant were produced in a baculovirus-ins
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0824769fd903e1218a955b477037c8a1
https://doi.org/10.1021/bi00480a021
https://doi.org/10.1021/bi00480a021
Autor:
Andrew C. Storer, Feng Ni, Wim F. Vranken, Marie-Claude Magny, Elisa de Miguel, Robert Dupras, Youlu Yu, John S. Mort, Nathalie Goudreau
Publikováno v:
FEBS letters. 429(1)
Selected fragments of the 62-residue proregion (or residues 1p–62p) of the cysteine protease cathepsin B were synthesized and their interactions with cathepsin B studied by use of proton NMR spectroscopy. Peptide fragments 16p–51p and 26p–51p e
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f9806becc5e0659596816e13015ee66e
https://pubmed.ncbi.nlm.nih.gov/9657374
https://pubmed.ncbi.nlm.nih.gov/9657374
The 49-kDa, nuclear inclusion a-like, viral protein genome-linked proteinase (VPg-Pro) of turnip mosaic potyvirus (TuMV) was expressed in Escherichia coli. The protein was produced in a soluble form at high levels and was active, as demonstrated by i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::62612deab242b01e8775b3a8f272691d
https://nrc-publications.canada.ca/eng/view/object/?id=97168b4a-9053-448a-8558-4069722e3527
https://nrc-publications.canada.ca/eng/view/object/?id=97168b4a-9053-448a-8558-4069722e3527
Publikováno v:
Biochemistry and cell biology = Biochimie et biologie cellulaire. 69(8)
The aim of this work was to compare the disappearance rate of human and rat intermediate density lipoproteins (IDL) using the rat liver perfusion system. Human and rat IDL were produced in vitro by incubating human or rat very low density lipoprotein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::bf9879f7f72ff886e028c3f4dbe5874e
https://pubmed.ncbi.nlm.nih.gov/1662053
https://pubmed.ncbi.nlm.nih.gov/1662053
Autor:
Andrew C. Storer, Daniel C. Tessier, Robert Ménard, David Y. Thomas, Céline Plouffe, Robert Dupras, Thierry Vernet, H E Khouri
Publikováno v:
Protein engineering. 4(3)
A mutant of papain, where an inter-domain hydrogen bond between the side chain hydroxyl group of a serine residue at position 176 and the side chain carbonyl oxygen of a glutamine residue at position 19 has been removed by site-directed mutagenesis,
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6f89ed71def74b738607538e2a1ad31b
https://pubmed.ncbi.nlm.nih.gov/1907009
https://pubmed.ncbi.nlm.nih.gov/1907009
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 796:277-284
Using two methods, we have compared the ability of three types of very-low-density lipoprotein (VLDL), namely, rat hepatic perfusate VLDL, rat serum VLDL and human plasma VLDL, to accept cholesteryl esters from human plasma high-density lipoprotein (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d96b486e21f7e2f06cb1a0416305a419
https://doi.org/10.1016/0005-2760(84)90128-0
https://doi.org/10.1016/0005-2760(84)90128-0