Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Robert C. Habbersett"'
Autor:
Travis A. Woods, Steven W. Graves, Robert C. Habbersett, John P. Nolan, Mark A. Naivar, David S. Sebba, Mark E. Wilder
Publikováno v:
Cytometry Part A. :979-989
Fully digital data acquisition systems for use in flow cytometry provide excellent flexibility and precision. Here, we demonstrate the development of a low cost, small, and low power digital flow cytometry data acquisition system using a single micro
Autor:
Larry A. Sklar, Bruce S. Edwards, Mark A. Naivar, John C. Martin, Robert C. Habbersett, James H. Jett, Jimmie D. Parson, James P. Freyer, Mark E. Wilder, Steven W. Graves, John P. Nolan
Publikováno v:
Cytometry Part A. :915-924
A digital signal processing (DSP)-based digital data acquisition system has been developed to support novel flow cytometry efforts. The system flexibility includes how it detects, captures, and processes event data. Custom data capture boards utilizi
Autor:
Robert C. Habbersett, James H. Jett, Xiaomei Yan, Thomas M. Yoshida, John P. Nolan, Babetta L. Marrone
Publikováno v:
Analytical Chemistry. 77:3554-3562
Rapid binding kinetics of SYTOX Orange stain with double-stranded DNA (dsDNA) was revealed on the DNA fragment sizing flow cytometer. We demonstrated for the first time that the dye molecules could be adsorbed onto the capillary surface and native DN
Autor:
Julia M. Cordek, John P. Nolan, Thomas M. Yoshida, Xiaomei Yan, Robert C. Habbersett, James H. Jett, Babetta L. Marrone
Publikováno v:
Analytical Biochemistry. 286:138-148
Accurate measurement of single DNA fragments by DNA fragment sizing flow cytometry (FSFC) depends upon precise, stoichiometric DNA staining by the intercalating dye molecules. In this study, we determined the binding characteristics of a commercially
Publikováno v:
Cytometry. 43:23-30
Background Flow cytometry is a valuable tool for the study of cellular and molecular interactions. We sought to develop instrumentation that would allow accurate and precise inline dynamic temperature control of flow cytometry samples in order to exp
Autor:
Dirk van Papendorp, Robert C. Habbersett, Yulin Shou, Bruce E. Lehnert, J.C. Seegers, Mona-Liza Lottering, Christina J.S. Grobler
Publikováno v:
The Journal of Steroid Biochemistry and Molecular Biology. 62:253-267
The endogenous metabolite, 2-methoxyestradiol (2ME), is an inhibitor of tubulin polymerization and is therefore toxic to dividing fast-growing tumor cells. Transformed cells are not equally susceptible to the effects of 2ME. In this study the effects
Autor:
John A. Steinkamp, Chiranjit Deka, Robert C. Habbersett, John C. Martin, L. S. Cram, Larry A. Sklar
Publikováno v:
Cytometry. 21:318-328
In frequency-domain lifetime spectroscopy, the apparent fluorescence lifetimes obtained from phase-shift measurements are independent of modulation frequency only in the special case of a single exponential fluorescence decay. For heterogeneous fluor
Publikováno v:
Cytometry. 21:223-229
Kinetic approaches are valuable tools for mechanistic studies of cell function. Flow cytometry is well suited to make sensitive kinetic measurements, but the time required to deliver mixed samples to the point of measurement (10-20 s in a conventiona
Autor:
Terri Gilbert Houghton, Larry A. Sklar, Richard P. Haugland, Robert C. Habbersett, Shawn P. Fay, Ed Pierson, Mark D. Domalewski, Richard J. Freer, Richard G. Posner, Natesa Muthukumaraswamy, James E. Whitaker
Publikováno v:
Cytometry. 15:148-153
Environmentally sensitive molecules have many potential cellular applications. We have investigated the utility of a pH sensitive ligand for the formyl peptide receptor, CHO-Met-Leu-Phe-Phe-Lys (SNAFL)-OH (SNAFL-seminaphtho-fluorescein), because in p
Publikováno v:
Review of Scientific Instruments. 62:2751-2764
An improved multilaser instrument has been developed for quantitative analysis and separation of biological cells and particles. Argon ion, krypton ion, and dye lasers are employed as excitation sources to sequentially illuminate cells labeled with m