Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Robert C Shields"'
Autor:
Robert C Shields, Alejandro R Walker, Natalie Maricic, Brinta Chakraborty, Simon A M Underhill, Robert A Burne
Publikováno v:
PLoS Pathogens, Vol 16, Iss 3, p e1008344 (2020)
A recent genome-wide screen identified ~300 essential or growth-supporting genes in the dental caries pathogen Streptococcus mutans. To be able to study these genes, we built a CRISPR interference tool around the Cas9 nuclease (Cas9Smu) encoded in th
Externí odkaz:
https://doaj.org/article/5684d65e60cf41ad99991ef62d12f82a
Publikováno v:
PLoS ONE, Vol 14, Iss 4, p e0211848 (2019)
Long-term monitoring of species assemblages provides a unique opportunity to test hypotheses regarding environmentally induced directional trajectories of freshwater species assemblages. We used 57 years of lockchamber fish rotenone and boat electrof
Externí odkaz:
https://doaj.org/article/6c8a03d33fe94f8f82d92025b272f5fe
Publikováno v:
PLoS ONE, Vol 14, Iss 6, p e0218915 (2019)
[This corrects the article DOI: 10.1371/journal.pone.0211848.].
Externí odkaz:
https://doaj.org/article/758bd87c06fc427cb6b6c4ecb572734b
Autor:
Kassapa Ellepola, Robert C. Shields, Jessica K. Kajfasz, Hua Zhang, Jose A. Lemos, Hui Wu, Zezhang T. Wen
Publikováno v:
mSphere, Vol 9, Iss 12 (2024)
ABSTRACT Our recent studies have shown that deficiency of MecA in Streptococcus mutans significantly affects cell division, growth, and biofilm formation. In this study, an in vitro mixed-species model, proteomics, and affinity pull-down assays were
Externí odkaz:
https://doaj.org/article/71f8cdb73c39499f85f55758f4727993
Autor:
Robert C Shields, Norehan Mokhtar, Michael Ford, Michael J Hall, J Grant Burgess, Mohamed Reda ElBadawey, Nicholas S Jakubovics
Publikováno v:
PLoS ONE, Vol 8, Iss 2, p e55339 (2013)
BackgroundThe persistent colonization of paranasal sinus mucosa by microbial biofilms is a major factor in the pathogenesis of chronic rhinosinusitis (CRS). Control of microorganisms within biofilms is hampered by the presence of viscous extracellula
Externí odkaz:
https://doaj.org/article/1c5df9a6d75c4ff79fe3e2af09c44761
Publikováno v:
Microbiology Spectrum, Vol 12, Iss 1 (2024)
ABSTRACT Arrayed mutant libraries are important resources that have advanced our understanding of bacterial genetics. Until recently, essential genes which are necessary for bacterial survival, and constitute ~10% of bacterial genes, were not include
Externí odkaz:
https://doaj.org/article/fa8e57461d98414ba99a762b75100845
Autor:
Alejandro R. Walker, Robert C. Shields
Publikováno v:
Frontiers in Genetics, Vol 13 (2022)
CRISPR-Cas is a bacterial immune system that restricts the acquisition of mobile DNA elements. These systems provide immunity against foreign DNA by encoding CRISPR spacers that help target DNA if it re-enters the cell. In this way, CRISPR spacers ar
Externí odkaz:
https://doaj.org/article/98189346dabb41bb8b9c8e9b9736d7eb
Geomorphology variables predict fish assemblages for forested and endorheic rivers of two continents
Autor:
Bud Mendsaikhan, Mark Pyron, Solongo Altangerel, Emily R. Arsenault, Amarbat Otgonganbat, Robert C. Shields, John Costello, Caleb C. Artz, Alain Maasri, James H. Thorp, Mario Minder
Publikováno v:
Ecology and Evolution
Ecology and Evolution, Vol 11, Iss 23, Pp 16745-16762 (2021)
Ecology and Evolution, Vol 11, Iss 23, Pp 16745-16762 (2021)
Stream fishes are restricted to specific environments with appropriate habitats for feeding and reproduction. Interactions between streams and surrounding landscapes influence the availability and type of fish habitat, nutrient concentrations, suspen
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a70422afd022a8b20fe3ef8d8ed50388
http://europepmc.org/articles/PMC8668727
http://europepmc.org/articles/PMC8668727
Autor:
Nadia Rostami, Robert C. Shields, Hannah J. Serrage, Catherine Lawler, Jane L. Brittan, Sufian Yassin, Halah Ahmed, Achim Treumann, Paul Thompson, Kevin J. Waldron, Angela H. Nobbs, Nicholas S. Jakubovics
Publikováno v:
Rostami, N, Shields, R C, Serrage, H J, Lawler, C, Brittan, J L, Yassin, S, Ahmed, H, Treumann, A, Thompson, P, Waldron, K J, Nobbs, A H & Jakubovics, N S 2022, ' Interspecies competition in oral biofilms mediated by Streptococcus gordonii extracellular deoxyribonuclease SsnA ', npj Biofilms and Microbiomes, vol. 8, no. 1, 96 . https://doi.org/10.1038/s41522-022-00359-z
Extracellular DNA (eDNA) is a key component of many microbial biofilms including dental plaque. However, the roles of extracellular deoxyribonuclease (DNase) enzymes within biofilms are poorly understood. Streptococcus gordonii is a pioneer colonizer
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a086b82984b038350b2db60d2de5b518
https://doi.org/10.1038/s41522-022-00359-z
https://doi.org/10.1038/s41522-022-00359-z
Publikováno v:
Microbiology (Reading, England). 168(10)
Integrative and conjugative elements (ICEs) are chromosomally encoded mobile genetic elements that can transfer DNA between bacterial strains. Recently, as part of efforts to determine hypothetical gene functions, we have discovered an important regu
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::1cc006a207057cfb0c262c15cdbf4e8a
https://pubmed.ncbi.nlm.nih.gov/36201342
https://pubmed.ncbi.nlm.nih.gov/36201342