Výsledky vyhledávání - "Robert C Boltz"

Akademický článek

Identifying and quantifying heterogeneity in high content analysis: application of heterogeneity indices to drug discovery.

Autor: Albert H Gough, Ning Chen, Tong Ying Shun, Timothy R Lezon, Robert C Boltz, Celeste E Reese, Jacob Wagner, Lawrence A Vernetti, Jennifer R Grandis, Adrian V Lee, Andrew M Stern, Mark E Schurdak, D Lansing Taylor

Publikováno v: PLoS ONE, Vol 9, Iss 7, p e102678 (2014)

One of the greatest challenges in biomedical research, drug discovery and diagnostics is understanding how seemingly identical cells can respond differently to perturbagens including drugs for disease treatment. Although heterogeneity has become an a

Externí odkaz: https://doaj.org/article/073440165ed34f8b8cced8d70b8f685a

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Fluorescent protein biosensors applied to microphysiological systems

Autor: Richard DeBiasio, Albert Gough, Nina Senutovitch, Robert C. Boltz, D. Lansing Taylor, Lawrence Vernetti

Publikováno v: Experimental Biology and Medicine. 240:795-808

This mini-review discusses the evolution of fluorescence as a tool to study living cells and tissues in vitro and the present role of fluorescent protein biosensors (FPBs) in microphysiological systems (MPSs). FPBs allow the measurement of temporal a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8bed656fb804865f7268ecd6743e256b
https://doi.org/10.1177/1535370215584934

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A human liver microphysiology platform for investigating physiology, drug safety, and disease models

Autor: D. Lansing Taylor, Richard DeBiasio, Lawrence Vernetti, Albert Gough, Nina Senutovitch, Tong Ying Shun, Robert C. Boltz

Publikováno v: Experimental biology and medicine (Maywood, N.J.). 241(1)

This paper describes the development and characterization of a microphysiology platform for drug safety and efficacy in liver models of disease that includes a human, 3D, microfluidic, four-cell, sequentially layered, self-assembly liver model (SQL-S

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0025d808d1447e70766f6076a01b3a36
https://pubmed.ncbi.nlm.nih.gov/26202373

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Characterization and Quantitation of NF-κB Nuclear Translocation Induced by Interleukin-1 and Tumor Necrosis Factor-α

Autor: Douglas K. Miller, Jack A. Schmidt, Gloria J.-F. Ding, Richard A. Rubin, Robert C. Boltz, Albert Gough, James J. Colaianne, Paul A. Fischer

Publikováno v: Journal of Biological Chemistry. 273:28897-28905

A new quantitative cytometric technique, termed the ArrayScanTM, is described and used to measure NF-kappaB nuclear translocation induced by interleukin (IL)-1 and tumor necrosis factor-alpha (TNFalpha). The amount of p65 staining is measured in both

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::24112d6b2a142ecc3c2d4e84102607f1
https://doi.org/10.1074/jbc.273.44.28897

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Single UV excitation of hoechst 33342 and ethidium bromide for simultaneous cell cycle analysis and viability determinations on in vitro cultures of murine B lymphocytes

Autor: Robert C. Boltz, Laurence B. Peterson, Paul A. Fischer, Linda S. Wicker

Publikováno v: Cytometry. 15:28-34

Assessment of DNA content by flow cytometry has largely depended on staining techniques which do not permit exclusion of dead cells from the data set. During studies of B cell activation in vitro, the large number of nonviable cells greatly affects t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8a1ef76123e13a8f65acbc26e76dffdf
https://doi.org/10.1002/cyto.990150106

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FK-506 and cyclosporin A inhibit highly similar signal transduction pathways in human T lymphocytes

Autor: John Siekierka, Nolan H. Sigal, C. Shirley Lin, Robert C. Boltz

Publikováno v: Cellular Immunology. 133:269-284

This report compares the ability of cyclosporin A and FK-506 to inhibit human T cell activation triggered via cell surface molecules that utilize different intracellular processes. We stimulated highly purified peripheral blood T lymphocytes with mit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f597c7cd39ba99a32d6effb97ddcfcf
https://doi.org/10.1016/0008-8749(91)90103-i

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Suppression of B cell activation by cyclosporin A, FK506 and rapamycin

Autor: Robert C. Boltz, Nolan H. Sigal, Victoria Matt, Elizabeth A. Nichols, Laurence B. Peterson, Linda S. Wicker

Publikováno v: European Journal of Immunology. 20:2277-2283

The effects of the immunosuppressants cyclosporin A (CsA), FK506 and rapamycin have been compared using murine B cells activated with a variety of mitogens. FK506 is a macrolide antibiotic that has been recently shown to inhibit T cell activation by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d7c68e210974bb2a010cc75845c5db9c
https://doi.org/10.1002/eji.1830201017

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Anti-proliferative activity of L-651,582 correlates with calcium-mediated regulation of nucleotide metabolism at phosphoribosyl pyrophosphate synthetase

Autor: Nancy Behrens, Robert C. Boltz, Donald Hupe

Publikováno v: Journal of Cellular Physiology. 144:457-466

L-651,582, 5-amino-(4-(4-chlorobenzoyl)-3,5-dichlorobenzyl)-1, 2,3-triazole-4-carboxamide, is an antiproliferative and antiparasitic agent which inhibits nucleotide metabolism in mammalian cells. The drug equivalently inhibited 3H-hypoxanthine, 14C-a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5dc16ca3d2d21cfad8a769d65fde1d20
https://doi.org/10.1002/jcp.1041440313

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