Zobrazeno 1 - 10
of 50
pro vyhledávání: '"Robert C, Shields"'
Autor:
Kassapa Ellepola, Robert C. Shields, Jessica K. Kajfasz, Hua Zhang, Jose A. Lemos, Hui Wu, Zezhang T. Wen
Publikováno v:
mSphere, Vol 9, Iss 12 (2024)
ABSTRACT Our recent studies have shown that deficiency of MecA in Streptococcus mutans significantly affects cell division, growth, and biofilm formation. In this study, an in vitro mixed-species model, proteomics, and affinity pull-down assays were
Externí odkaz:
https://doaj.org/article/71f8cdb73c39499f85f55758f4727993
Publikováno v:
Microbiology Spectrum, Vol 12, Iss 1 (2024)
ABSTRACT Arrayed mutant libraries are important resources that have advanced our understanding of bacterial genetics. Until recently, essential genes which are necessary for bacterial survival, and constitute ~10% of bacterial genes, were not include
Externí odkaz:
https://doaj.org/article/fa8e57461d98414ba99a762b75100845
Autor:
Nadia Rostami, Robert C. Shields, Hannah J. Serrage, Catherine Lawler, Jane L. Brittan, Sufian Yassin, Halah Ahmed, Achim Treumann, Paul Thompson, Kevin J. Waldron, Angela H. Nobbs, Nicholas S. Jakubovics
Publikováno v:
npj Biofilms and Microbiomes, Vol 8, Iss 1, Pp 1-13 (2022)
Abstract Extracellular DNA (eDNA) is a key component of many microbial biofilms including dental plaque. However, the roles of extracellular deoxyribonuclease (DNase) enzymes within biofilms are poorly understood. Streptococcus gordonii is a pioneer
Externí odkaz:
https://doaj.org/article/b8132f7fb87e4172a56a6d890dc4c643
Autor:
Alejandro R. Walker, Robert C. Shields
Publikováno v:
Frontiers in Genetics, Vol 13 (2022)
CRISPR-Cas is a bacterial immune system that restricts the acquisition of mobile DNA elements. These systems provide immunity against foreign DNA by encoding CRISPR spacers that help target DNA if it re-enters the cell. In this way, CRISPR spacers ar
Externí odkaz:
https://doaj.org/article/98189346dabb41bb8b9c8e9b9736d7eb
Autor:
Robert C Shields, Alejandro R Walker, Natalie Maricic, Brinta Chakraborty, Simon A M Underhill, Robert A Burne
Publikováno v:
PLoS Pathogens, Vol 16, Iss 3, p e1008344 (2020)
A recent genome-wide screen identified ~300 essential or growth-supporting genes in the dental caries pathogen Streptococcus mutans. To be able to study these genes, we built a CRISPR interference tool around the Cas9 nuclease (Cas9Smu) encoded in th
Externí odkaz:
https://doaj.org/article/5684d65e60cf41ad99991ef62d12f82a
Autor:
Simon A. M. Underhill, Robert C. Shields, Justin R. Kaspar, Momin Haider, Robert A. Burne, Stephen J. Hagen
Publikováno v:
mSphere, Vol 4, Iss 1 (2019)
Externí odkaz:
https://doaj.org/article/2a5df97fb0b748fd9e6308a72a0308a2
Publikováno v:
PLoS ONE, Vol 14, Iss 4, p e0211848 (2019)
Long-term monitoring of species assemblages provides a unique opportunity to test hypotheses regarding environmentally induced directional trajectories of freshwater species assemblages. We used 57 years of lockchamber fish rotenone and boat electrof
Externí odkaz:
https://doaj.org/article/6c8a03d33fe94f8f82d92025b272f5fe
Publikováno v:
PLoS ONE, Vol 14, Iss 6, p e0218915 (2019)
[This corrects the article DOI: 10.1371/journal.pone.0211848.].
Externí odkaz:
https://doaj.org/article/758bd87c06fc427cb6b6c4ecb572734b
Autor:
Simon A. M. Underhill, Robert C. Shields, Justin R. Kaspar, Momin Haider, Robert A. Burne, Stephen J. Hagen
Publikováno v:
mSphere, Vol 3, Iss 5 (2018)
ABSTRACT Entry into genetic competence in streptococci is controlled by ComX, an alternative sigma factor for genes that enable the import of exogenous DNA. In Streptococcus mutans, the immediate activator of comX is the ComRS quorum system. ComS is
Externí odkaz:
https://doaj.org/article/4ea79d5a5ea8453d9952e74a3f1e71ac
Publikováno v:
mSphere, Vol 3, Iss 1 (2018)
ABSTRACT Transposon mutagenesis coupled with next-generation DNA sequencing (Tn-seq) is a powerful tool for discovering regions of the genome that are required for the survival of bacteria in different environments. We adapted this technique to the d
Externí odkaz:
https://doaj.org/article/f6bb3f099fde41e29ed284e210c02913