Zobrazeno 1 - 10
of 38
pro vyhledávání: '"Robert B. Gunn"'
Autor:
Robert B. Gunn, Shelley D. Kozlowski, Young Hee Kim, Tekla D. Smith, Jeff M. Sands, John J. Doran, Janet D. Klein
Publikováno v:
American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 290:R1446-R1459
Mammalian urea transporters are facilitated membrane transport proteins belonging to two families, UT-A and UT-B. They are best known for their role of maintaining the renal inner medullary urinary concentrating gradient. Urea transporters have also
Publikováno v:
American Journal of Physiology-Cell Physiology. 286:C1264-C1270
Progress in understanding the cell biology of urea transporter proteins has been hampered by the lack of an appropriate cell culture system. The goal of this study was to create a polarized epithelial cell line that stably expresses the largest of th
Autor:
Makoto Uchikawa, Diane L. Cope, Rui-jun Pan, Robert B. Gunn, Lesley J. Bruce, Michael J. A. Tanner, Richard J. Cherry
Publikováno v:
Journal of Biological Chemistry. 279:2414-2420
We have studied the properties of band 3 in different glycophorin A (GPA)-deficient red cells. These red cells lack either both GPA and glycophorin B (GPB) (M(k)M(k) cells) or GPA (En(a-) cells) or contain a hybrid of GPA and GPB (MiV cells). Sulfate
Autor:
Sammy Elmariah, Robert B. Gunn
Publikováno v:
American Journal of Physiology-Cell Physiology. 285:C446-C456
The molecular basis for Na/Li exchange is unknown. Li can be transported by the Na pump, anion exchanger (AE1), a background leak, and the Na/Li exchanger. In vivo the intraerythrocyte concentration of Li results from the balance of passive entry, mo
Publikováno v:
Kidney International. 61(3):995-1002
Down-regulation of urea transporters in the renal inner medulla of lithium-fed rats. Background Lithium is commonly used to treat bipolar psychiatric disorders but can cause reduced urine concentrating ability. Methods To test whether lithium alters
Autor:
Janet D. Klein, Richard T. Timmer, Jill W. Verlander, Jeff M. Sands, John J. Doran, Serena M. Bagnasco, Robert B. Gunn
Publikováno v:
American Journal of Physiology-Cell Physiology. 281:C1318-C1325
A new polyclonal antibody to the human erythrocyte urea transporter UT-B detects a broad band between 45 and 65 kDa in human erythrocytes and between 37 and 51 kDa in rat erythrocytes. In human erythrocytes, the UT-B protein is the Kidd (Jk) antigen,
Autor:
Robert B. Gunn, Richard T. Timmer
Publikováno v:
The Journal of General Physiology
The kinetics of sodium-stimulated phosphate flux and phosphate-stimulated sodium flux in human red cells have been previously described (Shoemaker, D.G., C.A. Bender, and R.B. Gunn. 1988. J. Gen. Physiol. 92:449–474). However, despite the identific
Autor:
Robert B. Gunn, Richard T. Timmer
Publikováno v:
American Journal of Physiology-Cell Physiology. 276:C66-C75
A permanent cell line with inducible expression of the human anion exchanger protein 1 (hAE1) was constructed in a derivative of human embryonic kidney cells (HEK-293). In the absence of the inducer, muristerone A, the new cell line had no detectable
Autor:
Robert B. Gunn, Richard T. Timmer
Publikováno v:
American Journal of Physiology-Cell Physiology. 274:C757-C769
The human renal Na-PO4cotransporter gene NaPi-3 was expressed in human embryonic kidney HEK-293 cells, and the transport characteristics were measured in cells transfected with a vector containing NaPi-3 or with the vector alone (sham transfected). T