Improved Sequence Analysis of Intact Proteins by Parallel Ion Parking during Electron Transfer Dissociation

Autor: Maria C. Panepinto, Scott A. Ugrin, John E. P. Syka, Christopher Mullen, Robert Anthony D’Ippolito, Jeffrey Shabanowitz, Donald F. Hunt, Elizabeth M. Duselis, A. Michelle English

Publikováno v: Anal Chem

Electron transfer dissociation (ETD) is an analytically useful tool for primary structure interrogation of intact proteins, but its utility is limited by higher-order reactions with the products. To inhibit these higher-order reactions, first-generat

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d1eb8a4ecd6def1e14570206d61d2418
https://doi.org/10.1021/acs.analchem.1c03652

Protamines from liverwort are produced by post-translational cleavage and C-terminal di-aminopropanelation of several male germ-specific H1 histones

Autor: Donald F. Hunt, Juan Ausió, Takashi Ueda, Dina L. Bai, Manjinder S. Cheema, Jeffrey Shabanowitz, Ciro Rivera-Casas, José M. Eirín-López, Robert Anthony D’Ippolito, Naoki Minamino, Harold E. Kasinsky

Publikováno v: J Biol Chem

Protamines are small, highly-specialized, arginine-rich, and intrinsically-disordered chromosomal proteins that replace histones during spermiogenesis in many organisms. Previous evidence supports the notion that, in the animal kingdom, these protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::33b4bd0d97248950edf64dbbf6e191c3
https://doi.org/10.1074/jbc.ra119.010316

Interlaboratory study for characterizing monoclonal antibodies by top-down and middle-down mass spectrometry

Autor: Sam Hughes, Nicolas L. Young, David Clarke, Frank Sobott, Anja Resemann, Kristina Srzentić, Kim F. Haselmann, Detlev Suckau, Dina L. Bai, Young Ah Goo, Christian Malosse, Mathieu Dupré, Sylvester M. Greer, Neil R. Quebbemann, Ziqing Lin, Jared B. Shaw, Alain Beck, Yury O. Tsybin, Stuart Pengelley, Timothy K. Toby, Lidong He, Paul O. Danis, Henrique S. Seckler, Robert Anthony D’Ippolito, Natalia Gasilova, Jeffrey N. Agar, Donald F. Hunt, Jennifer S. Brodbelt, Matthew V. Holt, Simone Nicolardi, Joseph A. Loo, Yuri E. M. van der Burgt, Nicholas D. Schmitt, Laure Menin, Robert J. Millikin, Ljiljana Paša-Tolić, David R. Goodlett, Mowei Zhou, Joshua D. Hinkle, Michael R. Shortreed, Christopher L. Hendrickson, Wendy Sandoval, Richa Sarin, Jeffrey Shabanowitz, Alan G. Marshall, Anton N. Kozhinov, Neil L. Kelleher, Chad R. Weisbrod, Lissa C. Anderson, Julia Chamot-Rooke, Yunqiu Chen, John R. Yates, Luca Fornelli, Konstantin O. Nagornov, Lloyd M. Smith, Sneha Chatterjee, Ying Ge, Sung Hwan Yoon, Jolene K. Diedrich, Norelle C. Wildburger

Publikováno v: Journal of The American Society for Mass Spectrometry
Journal of The American Society for Mass Spectrometry, Springer Verlag (Germany), 2020, 31 (9), pp.1783-1802. ⟨10.1021/jasms.0c00036⟩
Journal of the American Society for Mass Spectrometry
Journal of the American Society for Mass Spectrometry, vol 31, iss 9
Journal of The American Society for Mass Spectrometry, 31(9), 1783-1802. AMER CHEMICAL SOC
J Am Soc Mass Spectrom
Journal of The American Society for Mass Spectrometry, 2020, 31 (9), pp.1783-1802. ⟨10.1021/jasms.0c00036⟩

International audience; The Consortium for Top-Down Proteomics (www.topdownproteomics.org) launched the present study to assess the current state of top-down mass spectrometry (TD MS) and middle-down mass spectrometry (MD MS) for characterizing monoc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ce93ac618ef03bef2381fe00d09bf9cf
https://hdl.handle.net/1887/3182316