Formation of carbohydrate-functionalised polystyrene and glass slides and their analysis by MALDI-TOF MS

Autor: Martin J. Weissenborn, Johannes W. Wehner, Christopher J. Gray, Robert Šardzík, Claire E. Eyers, Thisbe K. Lindhorst, Sabine L. Flitsch

Publikováno v: Beilstein Journal of Organic Chemistry, Vol 8, Iss 1, Pp 753-762 (2012)

Glycans functionalised with hydrophobic trityl groups were synthesised and adsorbed onto polystyrene and glass slides in an array format. The adsorbed glycans could be analysed directly on these minimally conducting surfaces by MALDI-TOF mass spectro

Externí odkaz: https://doaj.org/article/26944d9e23cb43759035cc69d3bb2e23

Label-Free Discovery Array Platform for the Characterization of Gly-can Binding Proteins and Glycoproteins

Autor: Mirja Hartmann, Sabine L. Flitsch, Santanu Mandal, Yassir A. Ahmed, Antonio Sánchez-Ruiz, Jerry Turnbull, Rebecca L. Miller, Kun Huang, Edward Pallister, Hannah N. Roberts, Robert Šardzík, Christopher J. Gray, Ivana Šardzíková, Juana Elizabeth Reyes Martinez, Peter Both, Claire E. Eyers

Publikováno v: Gray, C, Sanchez-Ruiz, A, Sardzikova, I, Ahmed, Y, Miller, R L, Reyes Martinez, J, Pallister, E, Huang, K, Both, P, Hartmann, M, Roberts, H, Sardzik, R, Mandal, S, Turnbull, J, Eyers, C & Flitsch, S 2017, ' Label-Free Discovery Array Platform for the Characterization of Gly-can Binding Proteins and Glycoproteins ', Analytical Chemistry, vol. 89, no. 8, pp. 4444-4451 . https://doi.org/10.1021/acs.analchem.6b04122
ANALYTICAL CHEMISTRY

The identification of carbohydrate-protein interactions is central to our understanding of the roles of cell-surface carbohydrates (the glycocalyx), fundamental for cell-recognition events. Therefore, there is a need for fast high-throughput biochemi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::153288e761ef7fc870053927a3989395
https://pure.manchester.ac.uk/ws/files/53145536/Gray_et_al_Anal_Chem_2017_Glycan_Arrays_Accepted_Manuscript.pdf

Chemoenzymatic Synthesis ofO-Mannosylpeptides in Solution and on Solid Phase

Autor: Stuart M. Haslam, Nicolas Laurent, Göran Widmalm, Anthony P. Green, Josef Voglmeir, Sabine L. Flitsch, Peter Both, Carolina Fontana, Rose Haddoub, Paola Grassi, Robert Šardzík, Martin J. Weissenborn

Publikováno v: Journal of the American Chemical Society

O-mannosyl glycans are known to play an important role in regulating the function of α-dystroglycan (α-DG), as defective glycosylation is associated with various phenotypes of congenital muscular dystrophy. Despite the well-established biological s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f51397db22ffcb3703bffa92336172db

Preparation of aminoethyl glycosides for glycoconjugation

Autor: Andrew Martin, Simon J. Webb, Sabine L. Flitsch, Robert Šardzík, Gavin T. Noble, Martin J. Weissenborn

Publikováno v: Beilstein Journal of Organic Chemistry
Beilstein Journal of Organic Chemistry, Vol 6, Iss 1, Pp 699-703 (2010)

The synthesis of a number of aminoethyl glycosides of cell-surface carbohydrates, which are important intermediates for glycoarray synthesis, is described. A set of protocols was developed which provide these intermediates, in a short number of steps

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::98158e6bca8cebe440c1864ccd5126a7
https://doi.org/10.3762/bjoc.6.81

Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing

Autor: Christopher J. Gray, Martin Rejzek, Claire E. Eyers, Anthony P. Green, D. Richardson, Sabine L. Flitsch, Josef Voglmeir, Carolina Fontana, Robert Šardzík, Martina Austeri, Peter Both, Robert A. Field, Göran Widmalm

Publikováno v: Nature Chemistry

Mass spectrometry is the primary analytical technique used to characterize the complex oligosaccharides that decorate cell surfaces. Monosaccharide building blocks are often simple epimers, which when combined produce diastereomeric glycoconjugates i

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e4b1edc46bcb801dc68326826c409891
http://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-100100