Preparative free-flow electrophoresis, a versatile technology complementing gradient centrifugation in the isolation of highly purified cell organelles

Autor: Alfred Völkl, Robert Wildgruber, Markus Islinger

Publikováno v: Electrophoresis. 39(18)

Free-flow electrophoresis (FFE) exploits differences in the overall charge of bio-particles separating cells, organelles, macromolecules, ions, etc. according to their distinct electrophoretic mobility and isoelectric point (pI) values. Indeed, aroun

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::974ae3df6610c078323fbc7ef155b8d5
https://pubmed.ncbi.nlm.nih.gov/29761848

Metabolic enzyme diversity in different human thyroid cell lines and their sensitivity to gravitational forces

Autor: Gerhard Weber, Albert Sickmann, Jessica Pietsch, Marcel Egli, Daniela Grimm, Robert Wildgruber, Manfred Infanger, Johann Bauer

Publikováno v: Pietsch, J, Sickmann, A, Weber, G, Bauer, J, Egli, M, Wildgruber, R, Infanger, M & Grimm, D G 2012, ' Metabolic enzyme diversity in different human thyroid cell lines and their sensitivity to gravitational forces ', Proteomics, vol. 12, no. 15-16, pp. 2539-46 . https://doi.org/10.1002/pmic.201200070

Many cancer cells show unique protein expression patterns. We used proteome technology including MS, free flow isoelectric focusing and Western blotting to determine current concentrations of metabolic enzymes in healthy and malignant human thyroid c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cd826723d3711c35d24eebe39eb7a368
https://doi.org/10.1002/pmic.201200070

Mammalian Proteasome Subpopulations with Distinct Molecular Compositions and Proteolytic Activities

Autor: Chenggong Zong, Aldrin V. Gomes, Ute Sukop, Peipei Ping, Robert Wildgruber, Gerhard Weber, Mikkel Nissum, Oliver Drews

Publikováno v: Molecular & Cellular Proteomics. 6:2021-2031

The proteasome-dependent protein degradation participates in multiple essential cellular processes. Modulation of proteasomal activities may alter cardiac function and disease phenotypes. However, cardiovascular studies reported thus far have yielded

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be8c12c663fe5c8bd528fb885d11e99f
https://doi.org/10.1074/mcp.m700187-mcp200

Free Flow Electrophoresis for Separation of Native Membrane Protein Complexes

Autor: Lutz A. Eichacker, Ute Sukop-Köppel, Robert Wildgruber, Gerhard Weber

Publikováno v: Methods in Molecular Biology ISBN: 9781493925490

This chapter describes the technology of free flow electrophoresis (FFE) and protocols to separate membrane protein complexes for proteome analysis. FFE is a highly versatile technology applied in the field of protein analysis. It is superior to nati

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https://doi.org/10.1007/978-1-4939-2550-6_29

Web-based two-dimensional database ofSaccharomyces cerevisiaeproteins using immobilized pH gradients from pH 6 to pH 12 and matrix-assisted laser desorption/ionization-time of flight mass spectrometry

Autor: Angelika Görg, Gerold Reil, Oliver Drews, Robert Wildgruber, Harun Parlar

Publikováno v: PROTEOMICS. 2:727-732

An image based two-dimensional (2-D) reference map of very alkaline yeast cell proteins was established by using immobilized pH gradients (IPG) up to pH 12 (IPG 6-12, IPG 9-12 and IPG 10-12) for 2-D electrophoresis and by using matrix-assisted laser

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https://doi.org/10.1002/1615-9861(200206)2:6<727::aid-prot727>3.0.co