Zobrazeno 1 - 10
of 367
pro vyhledávání: '"Robert, Tjian"'
Autor:
Nike Walther, Sathvik Anantakrishnan, Thomas G.W. Graham, Gina M. Dailey, Robert Tjian, Xavier Darzacq
Publikováno v:
Cell Reports, Vol 43, Iss 11, Pp 114914- (2024)
Summary: Lineage transcription factors (TFs) provide one regulatory level of differentiation crucial for the generation and maintenance of healthy tissues. To probe TF function by measuring their dynamics during adult intestinal homeostasis, we estab
Externí odkaz:
https://doaj.org/article/b193fa916d574acd963b26bdf2b9e090
Publikováno v:
eLife, Vol 13 (2024)
Externí odkaz:
https://doaj.org/article/33c440eff80f480fb68596378006e930
Publikováno v:
eLife, Vol 11 (2022)
Transcription factors (TFs) are classically attributed a modular construction, containing well-structured sequence-specific DNA-binding domains (DBDs) paired with disordered activation domains (ADs) responsible for protein-protein interactions target
Externí odkaz:
https://doaj.org/article/db180d29f34047c3bda457b3b6de0993
Publikováno v:
eLife, Vol 11 (2022)
Single-particle tracking (SPT) directly measures the dynamics of proteins in living cells and is a powerful tool to dissect molecular mechanisms of cellular regulation. Interpretation of SPT with fast-diffusing proteins in mammalian cells, however, i
Externí odkaz:
https://doaj.org/article/732963cee82c4d2482f9ddccbd79c962
Publikováno v:
eLife, Vol 11 (2022)
Single-molecule imaging provides a powerful way to study biochemical processes in live cells, yet it remains challenging to track single molecules while simultaneously detecting their interactions. Here, we describe a novel property of rhodamine dyes
Externí odkaz:
https://doaj.org/article/fdebf4b0ce4d48e9b56210b11f475b52
Autor:
Ina Hollerer, Juliet C. Barker, Victoria Jorgensen, Amy Tresenrider, Claire Dugast-Darzacq, Leon Y. Chan, Xavier Darzacq, Robert Tjian, Elçin Ünal, Gloria A. Brar
Publikováno v:
G3: Genes, Genomes, Genetics, Vol 9, Iss 4, Pp 1045-1053 (2019)
We recently described an unconventional mode of gene regulation in budding yeast by which transcriptional and translational interference collaborate to down-regulate protein expression. Developmentally timed transcriptional interference inhibited pro
Externí odkaz:
https://doaj.org/article/fbddfaee35014676a9c0c782a5c6f2d3
Autor:
Thomas G W Graham, Claire Dugast-Darzacq, Gina M Dailey, Xammy H Nguyenla, Erik Van Dis, Meagan N Esbin, Abrar Abidi, Sarah A Stanley, Xavier Darzacq, Robert Tjian
Publikováno v:
PLoS ONE, Vol 16, Iss 2, p e0246647 (2021)
Re-opening of communities in the midst of the ongoing COVID-19 pandemic has ignited new waves of infections in many places around the world. Mitigating the risk of reopening will require widespread SARS-CoV-2 testing, which would be greatly facilitat
Externí odkaz:
https://doaj.org/article/d2e00b68e6d249dcbc99fedaffd42153
Publikováno v:
Bio-Protocol, Vol 10, Iss 4 (2020)
Accurate abundance measurements of cellular proteins are required to achieve a quantitative and predictive understanding of any biological process inside the cell. Existing methods to determine absolute protein abundances are labor-intensive and/or r
Externí odkaz:
https://doaj.org/article/0b6dec14823b4a9292d476ce8c09d600
Publikováno v:
Bio-Protocol, Vol 10, Iss 4 (2020)
Protein-protein interactions constitute the molecular foundations of virtually all biological processes. Co-immunoprecipitation (CoIP) experiments are probably the most widely used method to probe both heterotypic and homotypic protein-protein intera
Externí odkaz:
https://doaj.org/article/c18839cc371d49919ba1bf7a813fc630
Autor:
Francesco M Piccolo, Zhe Liu, Peng Dong, Ching-Lung Hsu, Elitsa I Stoyanova, Anjana Rao, Robert Tjian, Nathaniel Heintz
Publikováno v:
eLife, Vol 8 (2019)
Methyl-CpG-binding-Protein 2 (MeCP2) is an abundant nuclear protein highly enriched in neurons. Here we report live-cell single-molecule imaging studies of the kinetic features of mouse MeCP2 at high spatial-temporal resolution. MeCP2 displays dynami
Externí odkaz:
https://doaj.org/article/483913f973bd4afd88a544e5341b3a8c