Evaluating the effect of glycation on lipase activity using boronate affinity chromatography and mass spectrometry

Autor: Guusje van Schaick, Sanne Pot, Olaf Schouten, Joost den Hartog, Michiel Akeroyd, Rob van der Hoeven, Wim Bijleveld, Nicolas Abello, Manfred Wuhrer, Maurien Olsthoorn, Elena Dominguez-Vega

Publikováno v: Food Chemistry. 421:136147

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4dffd1e9a28062d685729b19116f80e5
https://doi.org/10.1016/j.foodchem.2023.136147

Native liquid chromatography and mass spectrometry to structurally and functionally characterize endo-xylanase proteoforms

Autor: Guusje van Schaick, Nadi el Hajjouti, Simone Nicolardi, Joost den Hartog, Romana Jansen, Rob van der Hoeven, Wim Bijleveld, Nicolas Abello, Manfred Wuhrer, Maurien M. A. Olsthoorn, Elena Domínguez-Vega

Publikováno v: International Journal of Molecular Sciences; Volume 23; Issue 3; Pages: 1307
International Journal of Molecular Sciences, 23(3). MDPI
International Journal of Molecular Sciences, Vol 23, Iss 1307, p 1307 (2022)

Xylanases are of great value in various industries, including paper, food, and biorefinery. Due to their biotechnological production, these enzymes can contain a variety of post-translational modifications, which may have a profound effect on protein

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::481bbf253f36555b07c335600d46e592
https://hdl.handle.net/1887/3513328

Searching for microbial protein over-expression in a complex matrix using automated high throughput MS-based proteomics tools

Autor: Paul Klaassen, Laurens Ekkelkamp, Kerstin Strupat, Thibaut José Wenzel, Tjeerd van Rij, Diana Gutker-Vermaas, Ed Smit, Marcel W. E. M. van Tilborg, Wim Plugge, Michiel Akeroyd, Jort Steven Johan Gerritsma, Rob van der Hoeven, Maurien M.A. Olsthoorn

Publikováno v: Journal of Biotechnology. 164:112-120

In the discovery of new enzymes genomic and cDNA expression libraries containing thousands of differential clones are generated to obtain biodiversity. These libraries need to be screened for the activity of interest. Removing so-called empty and red

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a70c44db73b7490ab9e9abf8f15c848d
https://doi.org/10.1016/j.jbiotec.2012.11.015

Metabolic Engineering toward Sustainable Production of Nylon-6

Autor: Roel A. L. Bovenberg, Petronella Catharina Raemakers-Franken, Wigard P. Kloosterman, Karin P. A. M. Kolen, Stefaan Marie André De Wildeman, Roland Van Der Stoel, Julia Knutova, Henk Noorman, Martin Schürmann, Margarida Temudo, Dennis K. Ninaber, Axel Christoph Trefzer, Rob van der Hoeven, Michiel Akeroyd, Liang Wu, Erwin Suir, Monika Müller, Stefan C. H. J. Turk, Ruud van der Pol, Leonie M. Raamsdonk

Publikováno v: ACS Synthetic Biology, 5(1), 65. American Chemical Society
ACS Synthetic Biology, 5(1), 65-73. AMER CHEMICAL SOC

Nylon-6 is a bulk polymer used for many applications. It consists of the non-natural building block 6-aminocaproic acid, the linear form of caprolactam. Via a retro-synthetic approach, two synthetic pathways were identified for the fermentative produ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5e898d55318daea44c73880bf4a5c7b8
https://pubmed.ncbi.nlm.nih.gov/26511532

A Non-Canonical NRPS Is Involved in the Synthesis of Fungisporin and Related Hydrophobic Cyclic Tetrapeptides in Penicillium chrysogenum

Autor: Roel A. L. Bovenberg, Noël Nicolaas Maria Elisabeth Van Peij, Olaf Leonardus Schouten, Peter P. Lankhorst, Marek J. Noga, Thomas Hankemeier, Rob van der Hoeven, Hazrat Ali, Rob J. Vreeken, Arnold J. M. Driessen, Marco Ries

Publikováno v: PLoS ONE
PLoS ONE, Vol 9, Iss 6, p e98212 (2014)
PLoS ONE, 9(6):e98212. PUBLIC LIBRARY SCIENCE

The filamentous fungus Penicillium chrysogenum harbors an astonishing variety of nonribosomal peptide synthetase genes, which encode proteins known to produce complex bioactive metabolites from simple building blocks. Here we report a novel non-canon

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e92b23ce03a65b2320356ae13e827e66
https://doi.org/10.1371/journal.pone.0098212