Výsledky vyhledávání - "Rob J. Frankenberg"

Chemical denaturation and elevated folding temperatures are required for wild-type activity and stability of recombinant Methanococcus jannaschii 20S proteasome

Autor: Douglas S. Clark, Hisao Yakota, Rob J. Frankenberg, Tina S. Hsu, Rosalind Kim

Publikováno v: Protein Science. 10:1887-1896

The 20S proteasome from the extreme thermophile Methanococcus jannaschii (Mj) was purified and sequenced to facilitate production of the recombinant proteasome in E. coli. The recombinant proteasome remained in solution at a purity level of 80-85% (a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cbd55759da47b320a6058ca2c0ebc6f2
https://doi.org/10.1110/ps.ps.05801

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Effect of temperature and pressure on the proteolytic specificity of the recombinant 20S proteasome from Methanococcus jannaschii

Autor: Maria Andersson, Douglas S. Clark, Rob J. Frankenberg

Publikováno v: Extremophiles : life under extreme conditions. 7(5)

The hydrolytic specificity of the recombinant 20S proteasome from the deep-sea thermophile Methanococcus jannaschii was evaluated toward oxidized insulin B-chain across a range of temperatures (35 degrees, 55 degrees, 75 degrees, and 90 degrees C) an

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ffb3752ed51aa9d475c2d33fe53ec4f3
https://pubmed.ncbi.nlm.nih.gov/12820035

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