Zobrazeno 1 - 7
of 7
pro vyhledávání: '"Rikou Tanaka"'
Publikováno v:
Biophysics
An approach of cell-free synthesis is presented for the functional expression of transmembrane proteins without the need of refolding. The transmembrane region of the pharaonis halobacterial transducer protein, pHtrII, was translated with various lar
Publikováno v:
FEBS Journal. 275:727-738
The molecular dissection of human MCM2, a constituent of MCM2-7 licensing factor complex, was performed to identify the region responsible for its biochemical activities. Partial digestion with trypsin dissected the MCM2 protein into a central region
Publikováno v:
Protein Science. 12:1216-1221
For high-throughput protein structural analysis, it is indispensable to develop a reliable protein overexpression system. Although many protein overexpression systems, such as that involving Escherichia coli cells, have been developed, the number of
Autor:
Shun-ichiro Iemura, Tohru Natsume, Masaki Matsumoto, Rikou Tanaka, Tomohisa Hatta, Shungo Adachi, Masae Homoto, Yusaku Hioki, Hiroshi Murakami, Hiroaki Suga, Keiichi I. Nakayama
Publikováno v:
Nucleic Acids Research
Low-density lipoprotein receptor (LDLR) mRNA is unstable, but is stabilized upon extracellular signal-regulated kinase (ERK) activation, possibly through the binding of certain proteins to the LDLR mRNA 3′-untranslated region (UTR), although the de
Publikováno v:
Analytical biochemistry. 386(2)
Here we report the first application of amino acid-type selective (AATS) isotope labeling of a recombinant protein secreted by Brevibacillus choshinensis for a nuclear magnetic resonance (NMR) study. To prepare the 15N-AATS-labeled protein, the trans
Autor:
Masato Shimizu, Yaeta Endo, Toshiyuki Kohno, Tomio Ogasawara, Rikou Tanaka, Eugene Hayato Morita
Publikováno v:
Journal of biomolecular NMR. 30(1)
For high-throughput protein structural analyses, it is indispensable to develop a reliable protein overexpression system. Although many protein overexpression systems, such as ones utilizing E. coli cells, have been developed, a lot of proteins funct
Autor:
Shungo Adachi, Masae Homoto, Rikou Tanaka, Yusaku Hioki, Hiroshi Murakami, Hiroaki Suga, Masaki Matsumoto, Keiichi I. Nakayama, Tomohisa Hatta, Shun-ichiro Iemura, Tohru Natsume
Publikováno v:
Nucleic Acids Research; Sep2014, Vol. 42 Issue 15, p1-13, 13p, 1 Black and White Photograph, 4 Graphs