Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level

Autor: Fei Wang, Peiwen Ding, Xue Liang, Xiangning Ding, Camilla Blunk Brandt, Evelina Sjöstedt, Jiacheng Zhu, Saga Bolund, Lijing Zhang, Laura P. M. H. de Rooij, Lihua Luo, Yanan Wei, Wandong Zhao, Zhiyuan Lv, János Haskó, Runchu Li, Qiuyu Qin, Yi Jia, Wendi Wu, Yuting Yuan, Mingyi Pu, Haoyu Wang, Aiping Wu, Lin Xie, Ping Liu, Fang Chen, Jacqueline Herold, Joanna Kalucka, Max Karlsson, Xiuqing Zhang, Rikke Bek Helmig, Linn Fagerberg, Cecilia Lindskog, Fredrik Pontén, Mathias Uhlen, Lars Bolund, Niels Jessen, Hui Jiang, Xun Xu, Huanming Yang, Peter Carmeliet, Jan Mulder, Dongsheng Chen, Lin Lin, Yonglun Luo

Publikováno v: Nature Communications, Vol 13, Iss 1, Pp 1-18 (2022)

Pigs are important large animal models for biomedical research. Here, the authors construct a single-cell landscape of pig tissues, unravelling the phenotypic heterogeneity of blood endothelial cells in adipose tissues and the evolutionally conserved

Externí odkaz: https://doaj.org/article/c21d50994fe24c6aa783a0e4f2146142

HIF1A Knockout by Biallelic and Selection-Free CRISPR Gene Editing in Human Primary Endothelial Cells with Ribonucleoprotein Complexes

Autor: Camilla Blunk Brandt, Sofie Vestergaard Fonager, János Haskó, Rikke Bek Helmig, Søren Degn, Lars Bolund, Niels Jessen, Lin Lin, Yonglun Luo

Publikováno v: Biomolecules, Vol 13, Iss 1, p 23 (2022)

Primary endothelial cells (ECs), especially human umbilical vein endothelial cells (HUVECs), are broadly used in vascular biology. Gene editing of primary endothelial cells is known to be challenging, due to the low DNA transfection efficiency and th

Externí odkaz: https://doaj.org/article/374b4325e1a346488594f1f56efc2086

Transplacental transfer of SARS-CoV-2 antibodies:a cohort study

Autor: Stine Yde Nielsen, Lars Henning Petersen, May Murra, Lone Hvidman, Rikke Bek Helmig, Jens Kjølseth Møller, Mohammed Rohi Khalil, Maria Kirkeby, Tine Brink Henriksen

Publikováno v: Nielsen, S Y, Pedersen, L H, Murra, M, Hvidman, L, Helmig, R B, Møller, J K, Khalil, M R, Kirkeby, M & Henriksen, T B 2023, ' Transplacental transfer of SARS-CoV-2 antibodies : a cohort study ', European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, vol. 42, no. 3, pp. 277-285 . https://doi.org/10.1007/s10096-023-04553-5

The purpose of this study was to examine the transfer rate of SARS-CoV-2 IgG antibodies in pregnancy and newborns. Two Danish labor wards screened all women for SARS-CoV-2 by PCR upon arrival. Women (n = 99) with a SARS-CoV-2 PCR–positive nasophary

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::972276bf08970c710c46089f76aeada9
https://pure.au.dk/portal/da/publications/transplacental-transfer-of-sarscov2-antibodies(3e2a76b2-daa9-42a2-aab4-242d511e3202).html

Improved pregnancy outcomes in systemic lupus erythematosus:A retrospective study of pregnancies from a single centre in Denmark from 2010-2020 compared with the period 1990-2010

Autor: Mette Gohr, Iris Marie Jakobsen, Rikke Bek Helmig, Kristian Stengaard-Pedersen, Trine Bay Laurberg, Anne Troldborg

Publikováno v: Gohr, M, Jakobsen, I M, Helmig, R B, Stengaard-Pedersen, K, Laurberg, T B & Troldborg, A 2022, ' Improved pregnancy outcomes in systemic lupus erythematosus : A retrospective study of pregnancies from a single centre in Denmark from 2010-2020 compared with the period 1990-2010 ', Lupus, vol. 31, no. 10, pp. 1202-1210 . https://doi.org/10.1177/09612033221107538
Gohr, M, Jakobsen, I M, Helmig, R B, Stengaard-Pedersen, K, Laurberg, T B & Troldborg, A 2022, ' Improved pregnancy outcomes in systemic lupus erythematosus : A retrospective study of pregnancies from a single centre in Denmark from 2010–2020 compared with the period 1990–2010 ', Lupus, vol. 31, no. 10, pp. 1202-1210 . https://doi.org/10.1177/09612033221107538

Objectives Over the past decades new international guidelines recommend that pregnant Systemic lupus erythematosus (SLE) patients are monitored closely in a multi-professional team throughout pregnancy. The importance of low disease activity before p

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41ca68330732f0328124c48fdc648167
https://vbn.aau.dk/da/publications/9149fbcb-6fb4-447c-9151-9b4374e4b864

Women's body mass index and oral administration of Misoprostol for induction of labor - A retrospective cohort study

Autor: Lone Hvidman, Lise Brogaard, Rikke Bek Helmig

Publikováno v: Helmig, R B, Brogaard, L & Hvidman, L 2021, ' Women's body mass index and oral administration of Misoprostol for induction of labor – A retrospective cohort study ', Obesity Research and Clinical Practice, vol. 15, no. 5, pp. 509-511 . https://doi.org/10.1016/j.orcp.2021.09.001

Pregnant women who are obese are at greater risk of pregnancy complications, including preeclampsia, gestational diabetes, and macrosomia, leading to an increased likelihood of induced labor. Little is known about pregnant women with obesity and the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::cce5ca5f88631f2738664bc593cdb0b4
https://pubmed.ncbi.nlm.nih.gov/34503935