Zobrazeno 1 - 10
of 85
pro vyhledávání: '"Rika Numano"'
Publikováno v:
Frontiers in Neuroscience, Vol 18 (2024)
DNA aptamers can bind specifically to biomolecules to modify their function, potentially making them ideal oligonucleotide therapeutics. Herein, we screened for DNA aptamer of melanopsin (OPN4), a blue-light photopigment in the retina, which plays a
Externí odkaz:
https://doaj.org/article/9b35f811bd6b48aaa6c21e06c6728938
Autor:
Rika Numano, Akihiro Goryu, Yoshihiro Kubota, Hirohito Sawahata, Shota Yamagiwa, Minako Matsuo, Tadahiro Iimura, Hajime Tei, Makoto Ishida, Takeshi Kawano
Publikováno v:
FEBS Open Bio, Vol 12, Iss 4, Pp 835-851 (2022)
Genetic modification to restore cell functions in the brain can be performed through the delivery of biomolecules in a minimally invasive manner into live neuronal cells within brain tissues. However, conventional nanoscale needles are too short (len
Externí odkaz:
https://doaj.org/article/8904ddec34774351bd08e76161145020
Publikováno v:
PLoS ONE, Vol 18, Iss 5, p e0285444 (2023)
We investigated gene electrotransfer using electrical short-circuiting via a cell suspension droplet in dielectric oil. An aqueous droplet of a few microliters placed between a pair of electrodes can be deformed by an intense DC electric field depend
Externí odkaz:
https://doaj.org/article/3b48e49edd2449cdabaec6c44c210532
Publikováno v:
Biomedicines, Vol 11, Iss 5, p 1463 (2023)
All organisms maintain an internal clock that matches the Earth’s rotation over a period of 24 h, known as the circadian rhythm. Previously, we established Period1 luciferase (Per1::luc) transgenic (Tg) mice in order to monitor the expression rhyth
Externí odkaz:
https://doaj.org/article/89fe2e8575b542ac9c32b328fa291043
Autor:
Yuki Watanabe, Hirohito Nihonyanagi, Rika Numano, Takayuki Shibata, Kazunori Takashima, Hirofumi Kurita
Publikováno v:
Sensors, Vol 22, Iss 7, p 2494 (2022)
Membrane permeabilization stimulated by high-voltage electric pulses has been used to deliver cell-impermeable exogenous molecules. The electric field effect on the cells depends on various experimental parameters, such as electric field strength, th
Externí odkaz:
https://doaj.org/article/4d979b51df03406789dba63a6d5d7b96
Autor:
Hirofumi Kurita, Hirohito Nihonyanagi, Yuki Watanabe, Kenta Sugano, Ryuto Shinozaki, Kenta Kishikawa, Rika Numano, Kazunori Takashima
Publikováno v:
PLoS ONE, Vol 15, Iss 12, p e0243361 (2020)
We have developed a novel methodology for the delivery of cell-impermeable molecules, based on electrical short-circuiting via a water droplet in dielectric oil. When a cell suspension droplet is placed between a pair of electrodes with an intense DC
Externí odkaz:
https://doaj.org/article/b71db753ddd64365b4b823e3a41e9ea4
Autor:
Tsutomu Sasaki, Rika Numano, Hiromi Yokota-Hashimoto, Sho Matsui, Naobumi Kimura, Hideyuki Takeuchi, Tadahiro Kitamura
Publikováno v:
Molecular Brain, Vol 11, Iss 1, Pp 1-15 (2018)
Abstract A high-fat diet (HFD) causes obesity by promoting excessive energy intake, and simultaneously, by disturbing the timing of energy intake. Restoring the feeding pattern is sufficient to prevent HFD-induced obesity in mice. However, the molecu
Externí odkaz:
https://doaj.org/article/a3a5ddb4a6504a2bba4e5ff8e830ca8a
Autor:
Hirofumi Kurita, Shota Takahashi, Atsushi Asada, Minako Matsuo, Kenta Kishikawa, Akira Mizuno, Rika Numano
Publikováno v:
PLoS ONE, Vol 10, Iss 12, p e0144254 (2015)
Electroporation is the most widely used transfection method for delivery of cell-impermeable molecules into cells. We developed a novel gene transfection method, water-in-oil (W/O) droplet electroporation, using dielectric oil and an aqueous droplet
Externí odkaz:
https://doaj.org/article/47cc82dfa7cc430db6e3a505fc0693c7
Autor:
Tomoaki Banno, Shuhei Tsuruhara, Yu Seikoba, Ryohei Tonai, Koji Yamashita, Shinnosuke Idogawa, Yuto Kita, Ko Suzuki, Yuki Yagi, Yuki Kondo, Rika Numano, Kowa Koida, Takeshi Kawano
Publikováno v:
ACS Nano. 16:10692-10700
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::7c3d12a0e501eda3fc50ca4e8c065502
https://doi.org/10.1021/acsnano.2c02399
https://doi.org/10.1021/acsnano.2c02399
Autor:
Hiroshi Kitamura, Takeshi Ishino, Rika Numano, Yoshinori Shimamoto, Hirofumi Kurita, Rikio Kirisawa
Publikováno v:
The Journal of Veterinary Medical Science
Instrument cost is a major problem for the transduction of DNA fragments and proteins into cells. Water-in-oil droplet electroporation (droplet-EP) was recently invented as a low-cost and effective method for the transfection of plasmids into culture
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f8b2c869d360645cfe8283b4701c35e4
https://doi.org/10.1292/jvms.19-0475
https://doi.org/10.1292/jvms.19-0475