Zobrazeno 1 - 10
of 15
pro vyhledávání: '"Rihab Boushaba"'
Autor:
Nassim Madi, Rihab Boushaba
Publikováno v:
Food Science and Technology Research. 23:679-688
Autor:
Rihab Boushaba, Spyridon Gerontas, Daniel G. Bracewell, H Baldascini, Nigel J. Titchener-Hooker
Publikováno v:
Biotechnology Progress. 27:1009-1017
The high resolution afforded by packed bed chromatography makes it an indispensable operation in the downstream processing of therapeutic molecules. Packed bed performance is however inherently susceptible to changes in feed stream characteristics an
Publikováno v:
Biotechnology Progress. 19:1176-1182
An alternative route for the production of polyclonal F(ab')(2) fragments that might be adopted for the facile preparation of antivenoms is assessed in this work. The method involves the digestion of whole serum by free pepsin, which results in reduc
Publikováno v:
Biotechnology Progress. 24:192-201
Scale-down models of individual operations are widely used in biopharmaceutical process development to obtain information about the performance of production-scale equipment on the basis of inexpensive and efficient laboratory-scale tests, for the pu
Publikováno v:
Process Biochemistry. 42:812-819
Dual fluorescence confocal laser scanning microscopy has been used to visualise the binding of a fluorescently labelled polyclonal ovine anti-fluorescein F(ab′) 2 antibody to immobilised fluorescein. The fluorescent ligand was immobilised on a Stre
Publikováno v:
Journal of Chromatography A. 1142:137-147
This work presents a systematic evaluation of the influence of lipids and casein on the performance of a chromatographic capture step for the recovery of a target protein from transgenic milk. Lactoperoxidase (LPO) was spiked at concentrations typica
Publikováno v:
Journal of Chemical Technology & Biotechnology. 82:871-881
BACKGROUND: The fouling impact of selected fouling species was assessed by utilising confocal scanning laser microscopy (CSLM) to image a packed chromatographic bed during operation. A custom-made flow cell was packed with Q Sepharose FF and loaded w
Publikováno v:
Biotechnology Progress. 23:858-865
Supercoiled circular (SC) plasmid DNA is often subjected to fluid stress in large-scale manufacturing processes. It is thus important to characterize the engineering environment within a particular unit operation as well as within the associated anci
Autor:
Rihab Boushaba, Guy W. J. Moss, Sorwar Choudhury, Alex Graham, Sun Chau Siu, Vithaya Topoyassakul, Nigel J. Titchener-Hooker
Publikováno v:
Biotechnology and Bioengineering. 95:714-723
Confocal scanning laser microscopy (CSLM) was used to visualise the spatial location of foulants during the fouling of Q Sepharose FF matrix in finite batch experiments and for examining the subsequent effectiveness of clean-in-place (CIP) treatments
Publikováno v:
Food and Bioproducts Processing. 80:88-97
Asimple process for the manufacture of polyclonal F(ab′)2 fragments that might be adopted for the facile preparation of antivenoms is described. The production of polyclonal F(ab′)2 fragments from serum commonly involves the initial purification