Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis

Autor: Tom van der Wel, Riet Hilhorst, Hans den Dulk, Tim van den Hooven, Nienke M. Prins, Joost A. P. M. Wijnakker, Bogdan I. Florea, Eelke B. Lenselink, Gerard J. P. van Westen, Rob Ruijtenbeek, Herman S. Overkleeft, Allard Kaptein, Tjeerd Barf, Mario van der Stelt

Publikováno v: Nature Communications, Vol 11, Iss 1, Pp 1-20 (2020)

Chemical tools to monitor drug-target engagement of endogenous enzymes are essential for preclinical target validation. Here, the authors present a chemical genetics strategy to study target engagement of endogenous kinases, achieving specific labeli

Externí odkaz: https://doaj.org/article/749ac4b664044b4d9100599885c8e063

Analysis of Jak2 catalytic function by peptide microarrays: the role of the JH2 domain and V617F mutation.

Autor: Arturo Sanz, Daniela Ungureanu, Tuija Pekkala, Rob Ruijtenbeek, Ivo P Touw, Riet Hilhorst, Olli Silvennoinen

Publikováno v: PLoS ONE, Vol 6, Iss 4, p e18522 (2011)

Janus kinase 2 (JAK2) initiates signaling from several cytokine receptors and is required for biological responses such as erythropoiesis. JAK2 activity is controlled by regulatory proteins such as Suppressor of Cytokine Signaling (SOCS) proteins and

Externí odkaz: https://doaj.org/article/69ae644367c84bc2b99c88a21596b32e

Time dependent effect of cold ischemia on the phosphoproteome and protein kinase activity in fresh-frozen colorectal cancer tissue obtained from patients

Autor: Niek Bastiaensen, Richard R. de Haas, Rosanne van den Oord, Mariette Labots, Adrienne van den Berg, Rik de Wijn, Peet T. G. A. Nooijen, Tineke E. Buffart, Thang V. Pham, Riet Hilhorst, Henk M.W. Verheul, Adriaan J. C. van den Brule, Rob Ruijtenbeek, Theo van der Leij, Hans Pruijt, Connie R. Jimenez, Sander R. Piersma, Henk L. Dekker

Publikováno v: Clinical Proteomics, 18
Buffart, T E, van den Oord, R A H M, van den Berg, A, Hilhorst, R, Bastiaensen, N, Pruijt, H F M, van den Brule, A, Nooijen, P, Labots, M, de Goeij-de Haas, R R, Dekker, H, Piersma, S R, Pham, T V, van der Leij, T, de Wijn, R, Ruijtenbeek, R, Jiménez, C R & Verheul, H M W 2021, ' Time dependent effect of cold ischemia on the phosphoproteome and protein kinase activity in fresh-frozen colorectal cancer tissue obtained from patients ', Clinical Proteomics, vol. 18, no. 1, 8 . https://doi.org/10.1186/s12014-020-09306-6
Clinical Proteomics, 18, 1
Clinical Proteomics
Clinical Proteomics, 18(1):8. BioMed Central Ltd.

Background Based on their potential to analyze aberrant cellular signaling in relation to biological function, kinase activity profiling in tumor biopsies by peptide microarrays and mass spectrometry-based phosphoproteomics may guide selection of pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::121f69f67b3b653c5163000ee13c5ea3
https://doi.org/10.1186/s12014-020-09306-6

Technological advancements to study cellular signaling pathways in inherited retinal degenerative diseases

Autor: François Paquet-Durand, Akanksha Roy, Tushar Tomar, John P. Groten, Riet Hilhorst

Publikováno v: Current Opinion in Pharmacology, 60, 102-110
Current Opinion in Pharmacology 60 (2021)

Inherited retinal degenerative diseases (IRDs) are rare neurodegenerative disorders with mutations in hundreds of genes leading to vision loss, primarily owing to photoreceptor cell death. This genetic diversity is impeding development of effective t

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8773915769cffd2ce358b554b63e62bc
https://research.wur.nl/en/publications/technological-advancements-to-study-cellular-signaling-pathways-i

KRSA: Network-based Prediction of Differential Kinase Activity from Kinome Array Data

Autor: Eduard Bentea, Faris Naji, Jennifer L. McGuire, Jaroslaw Meller, Nawshaba Nawreen, Riet Hilhorst, Khaled Alganem, Robert E. McCullumsmith, Erica A K DePasquale

MotivationPhosphorylation by serine-threonine and tyrosine kinases is critical for determining protein function. Array-based approaches for measuring multiple kinases allow for the testing of differential phosphorylation between conditions for distin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::eb655f0014b36d755106119fc86ff188
https://doi.org/10.1101/2020.08.26.268581