High-Throughput Screening Assay for Detecting Drug-Induced Changes in Synchronized Neuronal Oscillations and Potential Seizure Risk Based on Ca2+ Fluorescence Measurements in Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neuronal 2D and 3D Cultures

Autor: Hua-Rong Lu, Manabu Seo, Mohamed Kreir, Tetsuya Tanaka, Rie Yamoto, Cristina Altrocchi, Karel van Ammel, Fetene Tekle, Ly Pham, Xiang Yao, Ard Teisman, David J. Gallacher

Publikováno v: Cells, Vol 12, Iss 6, p 958 (2023)

Drug-induced seizure liability is a significant safety issue and the basis for attrition in drug development. Occurrence in late development results in increased costs, human risk, and delayed market availability of novel therapeutics. Therefore, the

Externí odkaz: https://doaj.org/article/30146c9b38b545e3a4445329880d9e44

Branched-chain Amino Acid Biosensing Using Fluorescent Modified Engineered Leucine/Isoleucine/Valine Binding Protein

Autor: Stefano Ferri, Akane Sakaguchi, Koji Sode, Rie Yamoto, Sakura Chino

Publikováno v: International Journal of Molecular Sciences
Volume 8
Issue 6
Pages 513-525
International Journal of Molecular Sciences, Vol 8, Iss 6, Pp 513-525 (2007)

A novel fluorescence sensing system for branched-chain amino acids (BCAAs) was developed based on engineered leucine/isoleucine/valine-binding proteins (LIVBPs) conjugated with environmentally sensitive fluorescence probes. LIVBP was cloned from Esch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c41626d6e86cfca07a07f337b9b33619