Automated Analysis of Flow Cytometry Data to Reduce Inter-Lab Variation in the Detection of Major Histocompatibility Complex Multimer-Binding T Cells

Autor: Natasja Wulff Pedersen, P. Anoop Chandran, Yu Qian, Jonathan Rebhahn, Nadia Viborg Petersen, Mathilde Dalsgaard Hoff, Scott White, Alexandra J. Lee, Rick Stanton, Charlotte Halgreen, Kivin Jakobsen, Tim Mosmann, Cécile Gouttefangeas, Cliburn Chan, Richard H. Scheuermann, Sine Reker Hadrup

Publikováno v: Frontiers in Immunology, Vol 8 (2017)

Manual analysis of flow cytometry data and subjective gate-border decisions taken by individuals continue to be a source of variation in the assessment of antigen-specific T cells when comparing data across laboratories, and also over time in individ

Externí odkaz: https://doaj.org/article/1b4a566cddc84e09ac4febc3dbb62543

A benchmark for evaluation of algorithms for identification of cellular correlates of clinical outcomes

Autor: Greg Finak, Geoffrey J. McLachlan, Sofie Van Gassen, Raphael Gottardo, Celine Vens, Peng Qiu, Pratip K. Chattopadhyay, Dong Tong, Maria Chikina, Kui Wang, Miron B. Kursa, Garry P. Nolan, Nima Aghaeepour, Tom Dhaene, Richard H. Scheuermann, Yvan Saeys, Yu Qian, Slawomir Walkowiak, Mehrnoush Malek, Bart N. Lambrecht, Rick Stanton, Ryan R. Brinkman, Tim R. Mosmann

Publikováno v: Cytometry Part A. 89:16-21

The Flow Cytometry: Critical Assessment of Population Identification Methods (FlowCAP) challenges were established to compare the performance of computational methods for identifying cell populations in multidimensional flow cytometry data. Here we r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::4e8bde5267c7673d4aad17ba9ca0b851
https://doi.org/10.1002/cyto.a.22732

Automated Analysis of Flow Cytometry Data to Reduce Inter-Lab Variation in the Detection of Major Histocompatibility Complex Multimer-Binding T Cells

Autor: Cliburn Chan, Kivin Jakobsen, Charlotte Halgreen, Rick Stanton, Natasja Wulff Pedersen, Sine Reker Hadrup, Cécile Gouttefangeas, P. Anoop Chandran, Jonathan Rebhahn, Scott R. White, Richard H. Scheuermann, Mathilde Dalsgaard Hoff, Tim R. Mosmann, Alexandra J. Lee, Yu Qian, Nadia Viborg Petersen

Publikováno v: Frontiers in Immunology
Pedersen, N W, Chandran, P A, Qian, Y, Rebhahn, J, Petersen, N V, Hoff, M D, White, S, Lee, A J, Stanton, R, Halgreen, C, Jakobsen, K, Mosmann, T, Gouttefangeas, C, Chan, C, Scheuermann, R H & Hadrup, S R 2017, ' Automated Analysis of Flow Cytometry Data to Reduce Inter-Lab Variation in the Detection of Major Histocompatibility Complex Multimer-Binding T Cells ', Frontiers in Immunology, vol. 8, 858 . https://doi.org/10.3389/fimmu.2017.00858
Frontiers in Immunology, Vol 8 (2017)

Manual analysis of flow cytometry data and subjective gate-border decisions taken by individuals continue to be a source of variation in the assessment of antigen-specific T cells when comparing data across laboratories, and also over time in individ

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dc4907d579120381004bac9390441b50
https://doi.org/10.3389/fimmu.2017.00858

Standardizing Flow Cytometry Immunophenotyping Analysis from the Human ImmunoPhenotyping Consortium

Autor: Rick Stanton, Raphael Gottardo, Florian Kern, Marc Langweiler, Greg Finak, Jafar Taghiyar, J. Philip McCoy, Virginia Pascual, Mehrnoush Malek, Bonnie B. Blomberg, Karolina Palucka, Yael Korin, Nikolas Pontikos, Nadia Terrazzini, Marcin L. Pekalski, Ryan R. Brinkman, Tim R. Mosmann, Khadir Raddassi, John A. Ramey, Holden T. Maecker, Yu Qian, Gerlinde Obermoser, Lesley Devine, Susanne Heck, Federica Villanova, Alain Diaz, Kui Wang, Robert B. Nussenblatt, Nima Aghaeepour, Frank Nestle, Maria Jaimes, Richard H. Scheuermann, Elaine F. Reed, Aaron Brandes

Publikováno v: Scientific Reports

Standardization of immunophenotyping requires careful attention to reagents, sample handling, instrument setup, and data analysis, and is essential for successful cross-study and cross-center comparison of data. Experts developed five standardized, e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5a0ec8f720a1136018762b1ad40230ba

Mapping cell populations in flow cytometry data for cross-sample comparison using the Friedman-Rafsky test statistic as a distance measure

Autor: Chiaowen, Hsiao, Mengya, Liu, Rick, Stanton, Monnie, McGee, Yu, Qian, Richard H, Scheuermann

Publikováno v: Cytometry

Flow cytometry (FCM) is a fluorescence‐based single‐cell experimental technology that is routinely applied in biomedical research for identifying cellular biomarkers of normal physiological responses and abnormal disease states. While many comput

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2b87f66cce2fdfcb1cba8eb735e4474c
https://pubmed.ncbi.nlm.nih.gov/26274018