Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Richard Y-H. Wang"'
Autor:
Shyh-Ching Lo, M. M. Hayes, Richard Y-H. Wang, Douglas J. Wear, Teresa Grandinetti, J. W.-K. Shih, Nianxiang Zou
Publikováno v:
FEMS immunology and medical microbiology. 44(3)
We examined Mycoplasma penetrans-specific antibodies in sera of five male homosexual AIDS patients from whom M. penetrans was isolated during the disease process. No consistent immune reaction pattern could be recognized in Western blot using whole c
Publikováno v:
Vaccine. 17(23-24)
Hepatitis C Virus (HCV) causes most cases of posttransfusion hepatitis. Chronic HCV infection is highly related to chronic hepatitis, cirrhosis and hepatocellular carcinoma. Current therapies are only minimally effective and no vaccine has been devel
Autor:
Keiji Mitamura, James Wai-Kuo Shih, Kazumasa Hiroishi, Minoru Shibata, Harvey J. Alter, Takayoshi Ito, Makoto Yoshiba, Richard Y-H Wang
Publikováno v:
Gastroenterology. 120:A556
Publikováno v:
Nucleic Acids Research. 14:1599-1614
A nuclear protein isolated from human placenta, methylated DNA-binding protein (MDBP), binds selectively to DNA enriched in 5-methylcytosine. We now demonstrate that MDBP is a sequence-specific, as well as methylation-specific, DNA-binding protein. F
Two sequence-specific endonucleases from Xanthomonas oryzae. Characterization and unusual properties
Autor:
Daniel Kuebbing, Richard Y.-H. Wang, Melanie Ehrlich, Michael B. Farber, Joseph G. Shedlarski
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis. 606:371-385
XorI and XorII, two sequence-specific endonucleases, have been partially purified from Xanthomas oryzae. XorI and XorII were shown to be isoschizomers of PstI and PvuI, respectively. X. oryzae is a particularly good source of this PvuI isoschizomer b
Publikováno v:
Nucleic Acids Research. 8:4777-4790
Sodium bisulfite is a mutagen which can specifically deaminate more than 96% of the cytosine residues in single-stranded DNA via formation of a 5,6-dihydrocytosine-6-sulfonate intermediate. Under the same reaction conditions, only 2-3% of the 5-methy
Publikováno v:
Nucleic Acids Research. 14:8387-8397
Methylated DNA-binding protein (MDBP) from human placenta is the first protein shown to bind specifically to certain DNA sequences only when they are methylated at cytosine residues. Among the sites recognized by MDBP is pB site 1, a pBR322-derived s
Publikováno v:
Analytical Biochemistry. 122:89-93
An enzymatic method has been developed to synthesize 5-methyldeoxycytidine triphosphate (m5dCTP) starting with dCMP and formaldehyde as substrates. Methylation is catalyzed by a dCMP methyltransferase in the presence of tetrahydrofolic acid. The resu
Autor:
Richard Y.-H. Wang, Melanie Ehrlich, Lan-Hsiang Huang, Rana Khan, Xian-Yang Zhang, Youwen Zhou
Publikováno v:
Nucleic Acids Research. 14:9843-9860
Methylated DNA-binding protein (MDBP) from human placenta recognizes specific DNA sequences containing 5-methylcytosine (m5C) residues. Comparisons of binding of various prokaryotic DNAs to MDBP indicate that m5CpG is present in the recognition sites
Publikováno v:
Nucleic Acids Research. 11:3087-3095
Previously, we found much tissue- or cell-specificity in the levels of 5-methylcytosine (m5C) in the total human genome as well as in DNA fractions resolved by reassociation kinetics. We now report that there were even greater differences in the m5C