Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Richard W. Hammond"'
Autor:
Michael W. Deem, Richard W. Hammond, Joel S. Bader, Steven A. Henck, Jonathan M. Rothberg, John W. Simpson
Publikováno v:
Applied Physics A. 75:275-278
We have fabricated a micron-scale device capable of transporting DNA oligomers using Brownian ratchets. The ratchet potential is generated by applying a voltage difference to interdigitated electrodes. Cycling between the charged state and a discharg
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d8626fe8bcd1be0664c86ba44a93f483
https://doi.org/10.1007/s003390201330
https://doi.org/10.1007/s003390201330
Autor:
Hidehiro Oana, Olivia de Carmejane, Michael D. Morris, Jeffrey Bonadio, Richard W. Hammond, Jeffrey J. Schwinefus, Shau Chun Wang
Publikováno v:
Macromolecules. 32:4625-4630
The electrophoretic migration of 40 and 90 kbp supercoiled (sc) double-stranded (ds) plasmid DNA in dilute and entangled solutions of linear hydroxyethyl cellulose (HEC, Mn = 438 800) was visualized by video fluorescence microscopy at 60−222 frames
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5891e0b17bf8c8491c36e270da339062
https://doi.org/10.1021/ma990129x
https://doi.org/10.1021/ma990129x
Autor:
Masao Doi, Richard W. Hammond, Jeffrey J. Schwinefus, Michael D. Morris, Shau Chun Wang, Hidehiro Oana
Publikováno v:
Analytical Chemistry. 70:574-579
Capillary electrophoresis in dilute and semidilute (slightly entangled) hydroxyethyl cellulose (HEC) is shown to separate linear double-stranded DNA (ds-DNA) and supercoiled plasmid DNA in the size range 1-16 thousand base pairs in 3 min. The mobilit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfd44607262fda95a09f6cf728dfcc6b
https://doi.org/10.1021/ac970938j
https://doi.org/10.1021/ac970938j
Publikováno v:
Electrophoresis. 17:1110-1114
Fluorescence resonance energy transfer (FRET) microscopy is used to observe structures of DNA aggregates formed during electrophoresis in ultradilute hydroxyethylcellulose (HEC). FRET gives evidence of aggregates with a tightly packed core and random
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::97ea9b9d8dea089e52f4bc46c692409b
https://doi.org/10.1002/elps.1150170621
https://doi.org/10.1002/elps.1150170621
Publikováno v:
Journal of Microcolumn Separations. 8:201-210
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::59615adc0fc410c524c08b026568adc8
https://doi.org/10.1002/(sici)1520-667x(1996)8:3<201::aid-mcs5>3.0.co
https://doi.org/10.1002/(sici)1520-667x(1996)8:3<201::aid-mcs5>3.0.co
Publikováno v:
Analytical Chemistry. 67:1132-1138
Video microscopy of nucleic acids (DNA) undergoing electrophoresis in hydroxyethyl cellulose (HEC) sieving buffers demonstrates previously unobserved shapechanging interactions between DNA and HEC molecules. We provide the first visual demonstration
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4af8ab3ed01b483bf4f9071a87fd4852
https://doi.org/10.1021/ac00102a018
https://doi.org/10.1021/ac00102a018
Autor:
James Bustillo, Richard W. Hammond, Michael W. Deem, Steven A. Henck, Joel S. Bader, John W. Simpson, Gregory T. Mulhern, Jonathan M. Rothberg, Gregory A. McDermott
We have micromachined a silicon-chip device that transports DNA with a Brownian ratchet that rectifies the Brownian motion of microscopic particles. Transport properties for a DNA 50mer agree with theoretical predictions, and the DNA diffusion consta
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::13e8c60917be8a83c78c558ce1784d9e
Publikováno v:
SPIE Proceedings.
Nucleic acid electrophoretic separations in unentangled water-soluble polymers are rapid and applicable over the length range 500 bp to greater than 1.5 Mbp, depending upon the polymer matrix chosen. We have been able to achieve high resolution separ
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::82c319d8e6bc30c6b79e863010123846
https://doi.org/10.1117/12.237609
https://doi.org/10.1117/12.237609
Publikováno v:
Analytical chemistry. 67(18)
Using fluorescence video microscopy, DNA electrophoretic behavior under field inversion conditions has been investigated in hydroxyethyl cellulose (HEC) solutions both above and below its entanglement limit. DNA conformational fluctuation periods are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f9d31ee9db2912a7b90c8f62005170c
https://pubmed.ncbi.nlm.nih.gov/8686887
https://pubmed.ncbi.nlm.nih.gov/8686887
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