The phosphorylated trimeric SOSS1 complex and RNA polymerase II trigger liquid-liquid phase separation at double-strand breaks

Autor: Qilin Long, Marek Sebesta, Katerina Sedova, Vojtech Haluza, Adele Alagia, Zhichao Liu, Richard Stefl, Monika Gullerova

Publikováno v: Cell Reports, Vol 42, Iss 12, Pp 113489- (2023)

Summary: Double-strand breaks (DSBs) are the most severe type of DNA damage. Previously, we demonstrated that RNA polymerase II (RNAPII) phosphorylated at the tyrosine 1 (Y1P) residue of its C-terminal domain (CTD) generates RNAs at DSBs. However, th

Externí odkaz: https://doaj.org/article/7c47db390a97410190d2f9062b9b5371

Phosphorylated trimeric SOSS1 complex and RNA polymerase II trigger liquid-liquid phase separation at double-strand breaks

Autor: Qilin Long, Marek Sebesta, Katerina Sedova, Vojtech Haluza, Adele Alagia, Zhichao Liu, Richard Stefl, Monika Gullerova

The most toxic forms of DNA damage are double-strand breaks (DSBs). We have previously shown that RNA polymerase II (RNAPII), phosphorylated at tyrosine 1 (Y1P) on the C- terminal domain, transcribes RNA at DSBs to promote efficient DNA repair. Howev

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::064618b4bbfaddc54f4e0f81f2330aff
https://doi.org/10.1101/2023.05.10.540130

Structural and functional basis of mammalian microRNA biogenesis by Dicer

Autor: David Zapletal, Eliska Taborska, Josef Pasulka, Radek Malik, Karel Kubicek, Martina Zanova, Christian Much, Marek Sebesta, Valeria Buccheri, Filip Horvat, Irena Jenickova, Michaela Prochazkova, Jan Prochazka, Matyas Pinkas, Jiri Novacek, Diego F. Joseph, Radislav Sedlacek, Carrie Bernecky, Dónal O’Carroll, Richard Stefl, Petr Svoboda

Publikováno v: Zapletal, D, Taborska, E, Pasulka, J, Malik, R, Kubicek, K, Zanova, M, Much, C, Sebesta, M, Buccheri, V, Horvat, F, Jenickova, I, Prochazkova, M, Prochazka, J, Pinkas, M, Novacek, J, Joseph, D F, Sedlacek, R, Bernecky, C, O'Carroll, D, Stefl, R & Svoboda, P 2022, ' Structural and functional basis of mammalian microRNA biogenesis by Dicer ', Molecular Cell, vol. 82, no. 21, pp. 4064-4079.e13 . https://doi.org/10.1016/j.molcel.2022.10.010

MicroRNA (miRNA) and RNA interference (RNAi) pathways rely on small RNAs produced by Dicer endonucleases. Mammalian Dicer primarily supports the essential gene-regulating miRNA pathway, but how it is specifically adapted to miRNA biogenesis is unknow

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6d7ba0dd8dca43acb8f9a0f66b38fecb
https://pubmed.ncbi.nlm.nih.gov/36332606

Cooperation between intrinsically disordered and ordered regions of Spt6 regulates nucleosome and Pol II CTD binding, and nucleosome assembly

Autor: Aiste Kasiliauskaite, Karel Kubicek, Tomas Klumpler, Martina Zanova, David Zapletal, Eliska Koutna, Jiri Novacek, Richard Stefl

Publikováno v: Nucleic acids research 50(10), 5961-5973 (2022). doi:10.1093/nar/gkac451

Nucleic acids research / Special publication 50(10), 5961 - 5973 (2022). doi:10.1093/nar/gkac451
Transcription elongation factor Spt6 associates with RNA polymerase II (Pol II) and acts as a histone chaperone, which promotes the reassembly of nu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa9ed38e5cc6c98e60fb529848e7c907

In vivo SELEX reveals novel sequence and structural determinants of Nrd1-Nab3-Sen1-dependent transcription termination

Autor: Odil, Porrua, Fruzsina, Hobor, Jocelyne, Boulay, Karel, Kubicek, Yves, D'Aubenton-Carafa, Rajani Kanth, Gudipati, Richard, Stefl, Domenico, Libri

Publikováno v: The EMBO journal. 31(19)

The Nrd1-Nab3-Sen1 (NNS) complex pathway is responsible for transcription termination of cryptic unstable transcripts and sn/snoRNAs. The NNS complex recognizes short motifs on the nascent RNA, but the presence of these sequences alone is not suffici

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::b98110888d9c9647fd3a3d6825a725d2
https://pubmed.ncbi.nlm.nih.gov/23032188