Zobrazeno 1 - 10
of 35
pro vyhledávání: '"Richard S. Hayward"'
Publikováno v:
Journal of Bacteriology. 177:5193-5196
Two hundred forty-five consecutive amino acids of the sigma 70 subunit of Escherichia coli RNA polymerase are not conserved in the homologous protein of Bacillus subtilis. We show that their deletion from a sigma 70-32 hybrid protein caused no severe
Autor:
Richard S. Hayward, Mary Logan, Helen Williamson, Brenda Grimes, Ashok Kumar, Stephen Wedgwood
Publikováno v:
Journal of Molecular Biology. 246:563-571
Most of the sigma (transcriptional initiation specificity) subunits of RNA polymerase, from a wide range of eubacteria, show strong elements of amino acid sequence similarity. There is evidence that two of the "conserved" regions, 2.4 and 4.2, are in
Autor:
Richard S. Hayward
Publikováno v:
Genetical Research. 75:123-125
Publikováno v:
Technical Tips Online. 3(1):141-143
Publikováno v:
Journal of molecular biology. 299(5)
Formation of an initiation-competent RNA polymerase-promoter complex involves DNA melting over a region of about 12 base-pairs, which includes the start site of transcription, thus enabling the template strand to base-pair with the initiating nucleos
Autor:
Kozo Makino, Brenda Grimes, Akira Ishihama, Richard A. Malloch, Nobuyuki Fujita, Richard S. Hayward, Ashok Kumar
Publikováno v:
Journal of molecular biology. 235(2)
The role of the sigma 70 subunit of Escherichia coli RNA polymerase in transcription activation by positive transcription factors was investigated. For this purpose, we constructed a nested set of E. coli rpoD deletion mutants generating carboxy-term
Autor:
Richard S. Hayward, Nobuyuki Fujita, Richard A. Malloch, Ashok Kumar, David A. Smillie, Akira Ishihama
Publikováno v:
Journal of molecular biology. 232(2)
It is known that Escherichia coli promoters having the major minus 10 consensus sequence TATAAT, but lacking significant resemblance to consensus in the minus 35 region, allow transcriptional initiation in vivo and in vitro if they have an additional
Publikováno v:
Journal of Bacteriology. 174:3826-3827
Publikováno v:
Nature. 249:519-522
The genes for at least two Escherichia coli RNA polymerase subunits are contained in a single operon.
Publikováno v:
Nucleic Acids Research. 12:5465-5470
To investigate the possibility that the unusual dominant rifampicin-resistance characteristic of the rifd18 allele of E. coli rpoB is due to a secondary, regulatory mutation, we have determined the nucleotide sequence of a 1.1 Kbp wild-type DNA fragm