Zobrazeno 1 - 10
of 43
pro vyhledávání: '"Richard N. Trethewey"'
Publikováno v:
The Plant journal : for cell and molecular biology. 15(5)
We have used top-down metabolic control analysis to investigate the control of carbon flux through potato (Solanum tuberosum) plants during tuberisation. The metabolism of the potato plant was divided into two blocks of reactions (the source and sink
Autor:
Yves Gibon, Manuela Günter, Hendrik Poorter, Fabien Porée, Oliver Bläsing, Björn Usadel, Mark Stitt, Beate Kamlage, Richard N. Trethewey, Melanie Höhne
Publikováno v:
Plant, Cell and Environment
Plant, Cell and Environment, Wiley, 2008, 31 (4), pp.518-547. ⟨10.1111/j.1365-3040.2007.01763.x⟩
Plant, Cell and Environment, Wiley, 2008, 31 (4), pp.518-547. ⟨10.1111/j.1365-3040.2007.01763.x⟩
This paper characterizes the transcriptional and metabolic response of a chilling-tolerant species to an increasingly large decrease of the temperature. Arabidopsis Col-0 was grown at 20 degrees C and transferred to 17, 14, 12, 10 or 8 degrees C for
Autor:
Björn Usadel, Melanie Höhne, Oliver Bläsing, Daniel Osuna, Manuela Günter, Rosa Morcuende, Mark Stitt, Wolf-Rüdiger Scheible, Richard N. Trethewey, Yves Gibon, Beate Kamlage
Publikováno v:
The Plant Journal. 49:463-491
Arabidopsis seedlings were subjected to 2 days of carbon starvation, and then resupplied with 15 mm sucrose. The transcriptional and metabolic response was analyzed using ATH1 arrays, real-time quantitative (q)RT-PCR analysis of >2000 transcription r
Publikováno v:
Nature Reviews Molecular Cell Biology. 5:763-769
The concept of metabolite profiling has been around for several decades, but only recent technical innovations have allowed metabolite profiling to be carried out on a large scale - with respect to both the number of metabolites measured and the numb
Autor:
Richard N. Trethewey
Publikováno v:
Current Opinion in Plant Biology. 7:196-201
The past decade has seen some impressive successes in the metabolic engineering of biotechnologically important plant pathways. However, plant metabolic engineering currently proceeds more by trial and error than by intelligent system design. A chang
Autor:
Eva Tauberger, Richard N. Trethewey, Alisdair R. Fernie, Lothar Willmitzer, Anna Swiedrych, Anna Lytovchenko
Publikováno v:
Plant Physiology and Biochemistry. 40:921-927
Here, we describe results observed on the simultaneous down-regulation of both isoforms of phosphoglucomutase (EC 5.4.2.2; PGM) within potato (Solanum tuberosum L.) plants. Transgenic plants were created that were inhibited in both activities using t
Publikováno v:
Plant Molecular Biology. 49:491-501
Potato plants (Solanum tuberosum L. cv. Desiree) transformed with sense and antisense constructs of a cDNA encoding the potato hexokinase 2 exhibited altered enzyme activities and expression of hexokinase 2 mRNA. Measurements of the maximum catalytic
Publikováno v:
Trends in Plant Science. 7:35-41
The major flux in potato tuber carbon metabolism is the conversion of sucrose through hexose phosphates to starch. The enzymes that mediate this pathway are well characterized, the genes that encode them have been cloned and transgenic plants have be
Autor:
Eva M. Farré, Peter Geigenberger, Axel Tiessen, Lothar Willmitzer, Ute Roessner, Richard N. Trethewey
Publikováno v:
Plant Physiology. 127:685-700
The compartmentation of metabolism in heterotrophic plant tissues is poorly understood due to the lack of data on metabolite distributions and fluxes between subcellular organelles. The main reason for this is the lack of suitable experimental method
Autor:
Ute Roessner, Lothar Willmitzer, Andrea Leisse, Richard N. Trethewey, Jens Lubeck, Alisdair R. Fernie
Publikováno v:
Plant Physiology and Biochemistry. 39:825-830
The aim of this work was to investigate the possibility of modulating different enzyme activities in an antagonistic manner using a single transgenic approach. To this end a yeast (Saccharomyces cerevisiae) cDNA encoding for a β-fructosidase (invert