Zobrazeno 1 - 10
of 217
pro vyhledávání: '"Richard L. Schowen"'
Autor:
Valentino J. Stella, Gautam Dalwadi, Justin T. Douglas, Antonio Lopalco, Sida Niu, Richard L. Schowen
Publikováno v:
Journal of Pharmaceutical Sciences. 105:705-713
The purpose of this work was to probe the rate and mechanism of rapid decarboxylation of pyruvic acid in the presence of hydrogen peroxide (H2O2) to acetic acid and carbon dioxide over the pH range 2-9 at 25 °C, utilizing UV spectrophotometry, high
Publikováno v:
Journal of Physical Organic Chemistry. 26:1066-1070
The glucose dehydrogenase of the mesophilic bacterium Bacillus megaterium (optimal growth around 35 °C) exhibits non-linear Eyring temperature dependences from 25 to 55 °C in its catalysis of the oxidation by hydride-transfer to NAD+ of the β-anom
Autor:
Richard L. Schowen
The chapter introduces or recalls the main ideas of enzyme kinetics in very brief form. The main elements of a theoretical determination by a QM/MM simulation of rate constants for an enzyme-catalysed reaction are enumerated and discussed and illustr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::19f466a81acb976bd8f4106f7eae54ec
https://doi.org/10.1039/9781782626831-00031
https://doi.org/10.1039/9781782626831-00031
Publikováno v:
Journal of Physical Organic Chemistry. 23:586-605
Arrhenius curves of selected hydrogen transfer reactions in organic molecules and enzymes are reviewed with the focus on systems exhibiting temperature-independent kinetic isotope effects. The latter can be rationalized in terms of a ‘pre-tunneling
Autor:
C. Russell Middaugh, Jianwen Fang, Ronald T. Borchardt, Krzysztof Kuczera, Sumin Cai, Richard L. Schowen, Qing-Shan Li
Publikováno v:
Nucleosides, Nucleotides & Nucleic Acids. 28:473-484
S-Adenosylhomocysteine (AdoHcy) hydrolases (SAHHs) from human sources (Hs-SAHHs) bind the cofactor NAD(+) more tightly than several parasitic SAHHs by around 1000-fold. This property suggests the cofactor binding site of this essential enzyme as a po
Publikováno v:
Zeitschrift für Physikalische Chemie. 222:1333-1347
The thermophilic archaeon Thermoplasma acidophilum, with an optimal growth temperature in the region of 60 °C, has evolved a D-glucose dehydrogenase, dependent on NADP+ and accepting only the β-anomer of D-glucose, that exhibits a temperature depen
Publikováno v:
Proteins: Structure, Function, and Bioinformatics. 71:131-143
S-Adenosyl-L-homocysteine hydrolase (SAHH) is an enzyme regulating intracellular methylation reactions. The homotetrameric SAHH exists in an open conformation in absence of substrate, while enzyme:inhibitor complexes crystallize in the closed conform
Autor:
Richard L. Schowen, Sumin Cai, Qing-Shan Li, Krzysztof Kuczera, Jianwen Fang, C. Russell Middaugh, Ronald T. Borchardt
Publikováno v:
Biochemistry. 47:4983-4991
The S-adenosyl- l-homocysteine (AdoHcy) hydrolases (SAHH) from Homo sapiens (Hs-SAHH) and from the parasite Trypanosoma cruzi (Tc-SAHH) are very similar in structure and catalytic properties but differ in the kinetics and thermodynamics of associatio
Autor:
Richard L. Schowen
Publikováno v:
Isotopes in Environmental and Health Studies. 43:1-16
Solvent isotope effect and β-secondary isotope effect studies of the steady-state reaction of the pyruvate decarboxylase of Saccharomyces cerevisiae (ScPDC), which gains its full activity in a process requiring some seconds, suggested a model that a
Autor:
Richard L. Schowen
Publikováno v:
Journal of Labelled Compounds and Radiopharmaceuticals. 50:1052-1062
The basic principles and illustrative applications to enzyme mechanisms of solvent isotope effects, with emphasis on the use of rate measurements in mixtures of protium and deuterium oxides (‘proton inventories’), are reviewed over a period sligh