Zobrazeno 1 - 10
of 444
pro vyhledávání: '"Richard J Roberts"'
Autor:
Zhiru Li, Jacqueline L Bruce, Barry Cohen, Caileigh V Cunningham, William E Jack, Katell Kunin, Bradley W Langhorst, Jacob Miller, Reynes A Moncion, Catherine B Poole, Prem K Premsrirut, Guoping Ren, Richard J Roberts, Nathan A Tanner, Yinhua Zhang, Clotilde K S Carlow
Publikováno v:
PLoS ONE, Vol 17, Iss 5, p e0268692 (2022)
Effective management of the COVID-19 pandemic requires widespread and frequent testing of the population for SARS-CoV-2 infection. Saliva has emerged as an attractive alternative to nasopharyngeal samples for surveillance testing as it does not requi
Externí odkaz:
https://doaj.org/article/92026c356e18426da597bb818ba0347a
Publikováno v:
PLoS ONE, Vol 16, Iss 5, p e0247541 (2021)
Single-molecule Real-Time (SMRT) sequencing can easily identify sites of N6-methyladenine and N4-methylcytosine within DNA sequences, but similar identification of 5-methylcytosine sites is not as straightforward. In prokaryotic DNA, methylation typi
Externí odkaz:
https://doaj.org/article/2900462d9e0a40dcb80924cc2589c2eb
Autor:
Iain A Murray, Yvette A Luyten, Alexey Fomenkov, Nan Dai, Ivan R Corrêa, William G Farmerie, Tyson A Clark, Jonas Korlach, Richard D Morgan, Richard J Roberts
Publikováno v:
PLoS ONE, Vol 16, Iss 7, p e0253267 (2021)
We report a new subgroup of Type III Restriction-Modification systems that use m4C methylation for host protection. Recognition specificities for six such systems, each recognizing a novel motif, have been determined using single molecule real-time D
Externí odkaz:
https://doaj.org/article/2f77b6fdc04f4e65b94bfac091b7ee0f
Autor:
Elena V Liyaskina, Nadezhda A Rakova, Alevtina A Kitykina, Valentina V Rusyaeva, Philip V Toukach, Alexey Fomenkov, Saulius Vainauskas, Richard J Roberts, Victor V Revin
Publikováno v:
PLoS ONE, Vol 16, Iss 7, p e0253482 (2021)
Paenibacillus spp. exopolysaccharides (EPSs) have become a growing interest recently as a source of biomaterials. In this study, we characterized Paenibacillus polymyxa 2020 strain, which produces a large quantity of EPS (up to 68 g/L),and was isolat
Externí odkaz:
https://doaj.org/article/1e18ed39829e40febaa23f6a0793ea59
Autor:
Simon Kasif, Richard J Roberts
Publikováno v:
PLoS Biology, Vol 18, Iss 11, p e3000999 (2020)
How do we scale biological science to the demand of next generation biology and medicine to keep track of the facts, predictions, and hypotheses? These days, enormous amounts of DNA sequence and other omics data are generated. Since these data contai
Externí odkaz:
https://doaj.org/article/4ba3b1e37bb04bf8a6be513099dacf3b
Autor:
Christopher D Johnston, Chelsey A Skeete, Alexey Fomenkov, Richard J Roberts, Susan R Rittling
Publikováno v:
PLoS ONE, Vol 12, Iss 9, p e0185234 (2017)
Prevotella intermedia, a major periodontal pathogen, is increasingly implicated in human respiratory tract and cystic fibrosis lung infections. Nevertheless, the specific mechanisms employed by this pathogen remain only partially characterized and po
Externí odkaz:
https://doaj.org/article/48fe3eb0c481446d85763e442d1bcb45
Autor:
Alexey Fomenkov, Zhiyi Sun, Deborah K Dila, Brian P Anton, Richard J Roberts, Elisabeth A Raleigh
Publikováno v:
PLoS ONE, Vol 12, Iss 6, p e0179853 (2017)
Here we characterize the modification-dependent restriction enzyme (MDE) EcoBLMcrX in vivo, in vitro and in its genomic environment. MDE cleavage of modified DNAs protects prokaryote populations from lethal infection by bacteriophage with highly modi
Externí odkaz:
https://doaj.org/article/9fbd402b65bd46b0bce69b299b9ac339
Autor:
Scott J Callahan, Yvette A Luyten, Yogesh K Gupta, Geoffrey G Wilson, Richard J Roberts, Richard D Morgan, Aneel K Aggarwal
Publikováno v:
PLoS Biology, Vol 14, Iss 4, p e1002442 (2016)
The creation of restriction enzymes with programmable DNA-binding and -cleavage specificities has long been a goal of modern biology. The recently discovered Type IIL MmeI family of restriction-and-modification (RM) enzymes that possess a shared targ
Externí odkaz:
https://doaj.org/article/35e0325c03de4884acbf1c4e9414666d
Autor:
Matthew J Blow, Tyson A Clark, Chris G Daum, Adam M Deutschbauer, Alexey Fomenkov, Roxanne Fries, Jeff Froula, Dongwan D Kang, Rex R Malmstrom, Richard D Morgan, Janos Posfai, Kanwar Singh, Axel Visel, Kelly Wetmore, Zhiying Zhao, Edward M Rubin, Jonas Korlach, Len A Pennacchio, Richard J Roberts
Publikováno v:
PLoS Genetics, Vol 12, Iss 2, p e1005854 (2016)
DNA methylation acts in concert with restriction enzymes to protect the integrity of prokaryotic genomes. Studies in a limited number of organisms suggest that methylation also contributes to prokaryotic genome regulation, but the prevalence and prop
Externí odkaz:
https://doaj.org/article/611a8e3186b0460990c4d6a70850c22d
Publikováno v:
PLoS ONE, Vol 11, Iss 8, p e0161499 (2016)
We examined and compared both the methylomes and the modification-related gene content of four sequenced strains of Bibersteinia trehalosi isolated from the nasopharyngeal tracts of Nebraska cattle with symptoms of bovine respiratory disease complex.
Externí odkaz:
https://doaj.org/article/e57dcba6778f42bc8162e3302c3272ab