Zobrazeno 1 - 10
of 21
pro vyhledávání: '"Richard F. Parrish"'
Publikováno v:
Analytical Biochemistry. 192:453-457
Horseradish peroxidase which had been aminated by periodate oxidation and reductive amination was purified by cation-exchange chromatography on S-Sepharose. Instead of the expected single peak of aminated enzyme, two distinct peaks of protein were el
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4ead0602e38542be115a1f173a51aa3b
https://doi.org/10.1016/0003-2697(91)90564-a
https://doi.org/10.1016/0003-2697(91)90564-a
Autor:
Thomas M. Li, Richard F. Parrish
Publikováno v:
Topics in Fluorescence Spectroscopy ISBN: 0306439549
In the past ten years, numerous applications of fluorescence methodsfor monitoring homogeneous and heterogeneous immunoassays have been reported. Advances in the design of fluorescent labels have prompted thedevelopment of various fluorescent immunoa
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b045a510bf13c16c55e1f4c347399220
https://doi.org/10.1007/0-306-47059-4_6
https://doi.org/10.1007/0-306-47059-4_6
Publikováno v:
Journal of Biological Chemistry. 257:3095-3099
A proacrosin conversion inhibitor present in boar spermatozoa has been purified and initially characterized. Purification methods included sequential acid extractions of washed spermatozoa at pH 4.0, pH 3.5, and pH 2.5 followed by successive gel filt
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3ec02935a9a723c11183eb7807ccaee0
https://doi.org/10.1016/s0021-9258(19)81078-5
https://doi.org/10.1016/s0021-9258(19)81078-5
Publikováno v:
Journal of Biological Chemistry. 256:5662-5668
Acrosin, an enzyme required for fertilization, is an endogenous proteinase associated with membranes of the sperm acrosome. Liposomes were utilized as a model system to evaluate the mode of association between highly purified boar acrosin and phospho
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::740c3678bbd8c9a07e63133dd1bad4c3
https://doi.org/10.1016/s0021-9258(19)69256-2
https://doi.org/10.1016/s0021-9258(19)69256-2
Autor:
Richard F. Parrish, Kenneth L. Polakoski, Frank M. Stevens, Richard R. Tidwell, J. Dieter Geratz
Publikováno v:
Archives of Biochemistry and Biophysics. 197:551-559
A number of amidino-substituted heterocycles were synthesized and examined for their inhibitory effect against arginine-directed esteroproteases, including human and bovine thrombin (EC 3.4.21.5), human factor Xa and plasmin (EC 3.4.21.7), bovine try
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::16cab8e4a66330734e695a21a079da50
https://doi.org/10.1016/0003-9861(79)90279-0
https://doi.org/10.1016/0003-9861(79)90279-0
Publikováno v:
Journal of Andrology. 1:89-95
Glycosaminoglycans obtained from shark cartilage, whale cartilage, porcine intestinal mucosa, porcine skin, and human umbilical cord were found to accelerate the in vitro conversion of highly purified boar sperm proacrosin Into mα-acrosin. Since non
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::c9ec47de3fd4a6e2e033590725e807e1
https://doi.org/10.1002/j.1939-4640.1980.tb00013.x
https://doi.org/10.1002/j.1939-4640.1980.tb00013.x
Publikováno v:
Biology of Reproduction. 17:417-422
Polyamines, especially spermine, were demonstrated to stimulate both the esterase activity and proteinase activity of acrosin, a critical enzyme in the fertilization process. The stimulation was demonstrated to result from an increase in the maximal
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5bc485b545fef3e989ac86c6662426c8
https://doi.org/10.1095/biolreprod17.3.417
https://doi.org/10.1095/biolreprod17.3.417
Publikováno v:
Biochemistry. 16:4824-4831
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fcd040c626ebaa475231a677a7b4f703
https://doi.org/10.1021/bi00641a011
https://doi.org/10.1021/bi00641a011
Publikováno v:
The Prostate. 4:189-193
Pilocarpine-stimulated canine prostatic secretions were subjected to gel filtration chromatography over Sephadex G-100 and Bio Gel P-4. Free zinc and the zinc in pilocarpine-stimulated canine prostatic secretions were observed to elute in the same fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e2f41adf3a4f62d463afc6c5c5e915ee
https://doi.org/10.1002/pros.2990040209
https://doi.org/10.1002/pros.2990040209
Publikováno v:
Proceedings of the National Academy of Sciences. 75:149-152
Aqueous dispersions of synthetic phospholipids, in the form of anionic, single bilayer vesicles, were observed to stimulate the appearance of acrosin esterase activity from its zymogen precursor, proacrosin. Enzymatic activity measurements, in parall
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0f63f0c4bfb9babe089cb72881001b86
https://doi.org/10.1073/pnas.75.1.149
https://doi.org/10.1073/pnas.75.1.149