Zobrazeno 1 - 10
of 114
pro vyhledávání: '"Richard E. Reeves"'
Autor:
Richard K. Firmin, Hilliary M. Killer, Richard E. Reeves, Giles J. Peek, Andrezj W. Sosnowski
Publikováno v:
ASAIO journal (American Society for Artificial Internal Organs : 1992). 48(5)
Silicone oxygenators are the standard devices used for Extracorporeal Life Support (ECLS), but they have some limitations. Microporous polypropylene hollow fiber oxygenators overcome many of these problems but, unfortunately, develop plasma leak. Pol
Publikováno v:
Biochemical Journal. 204:191-199
Isotope studies indicate that hexose-to-pentose interconversion by axenic Entamoeba histolytica conserves the C-1 and C-6 hexose carbon atoms. Transketolase was readily identified in amoebal extracts, and transaldolase could not be demonstrated. Howe
Publikováno v:
The American Journal of Tropical Medicine and Hygiene. 32:976-979
Axenically grown Entamoeba histolytica was found to contain adenosine kinase. This organism lacks de novo purine biosynthetic pathways. Adenosine kinase provides the amoeba with a method for salvaging adenosine from ingested nucleosides or from degra
Publikováno v:
Experimental Parasitology. 49:83-88
A significant portion of the nonheme iron and acid-labile sulfide of axenically grown Entamoeba histolytica was obtained in a colored, low molecular weight protein fraction from centrifuged amoeba homogenates following gel filtration. The colored com
Autor:
Hsin-sheng Lo, Richard E. Reeves
Publikováno v:
Experimental Parasitology. 47:180-184
The individual flavin species of axenic Entamoeba histolytica were assayed: separated riboflavin was assayed by the lumiflavin method; flavin-adenine dinucleotide (FAD), by an enzymatic method; flavin mononucleotide (FMN) was calculated from the diff
Publikováno v:
Journal of Biological Chemistry. 249:7737-7741
The major enzyme of Entamoeba histolytica which produces fructose 1,6-diphosphate from fructose 6-phosphate utilizes inorganic pyrophosphate as phosphate donor. In its reverse reaction the enzyme utilizes orthophosphate and fructose diphosphate to yi
Publikováno v:
Journal of Biological Chemistry. 252:726-731
Under anaerobic conditions, cells of Entamoeba histolytica grown with bacteria produce H2 and acetate while cells grown axenically produce neither. Aerobically, acetate is produced and O2 is consumed by amebae from either type of cells. Centrifuged e
Autor:
Richard E. Reeves, Hsin-sheng Lo
Publikováno v:
The American Journal of Tropical Medicine and Hygiene. 28:194-197
Riboflavin was found to be essential for the cultivation of axenic Entamoeba histolytica. This is the first demonstration of a flavin requirement by the organism. Panmede, the principal source of flavins in the axenic medium, was treated with activat
Autor:
Richard E. Reeves, Hsin-Sheng Lo
Publikováno v:
Molecular and Biochemical Parasitology. 2:23-30
Amebal NADPH:flavin oxidoreductase was purified to apparent homogeneity. Molecular weights of 40 000 and 38 000 were estimated by gel filtration and by sodium dodecyl sulfate polyacrylamide gel electrophoresis, respectively, indicating that the enzym