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of 5
pro vyhledávání: '"Richard C Silva"'
Autor:
Richard C Silva, Martina Dautel, Bruno M Di Genova, David C Amberg, Beatriz A Castilho, Evelyn Sattlegger
Publikováno v:
PLoS ONE, Vol 10, Iss 7, p e0131070 (2015)
The Saccharomyces cerevisiae protein Yih1, when overexpressed, inhibits the eIF2 alpha kinase Gcn2 by competing for Gcn1 binding. However, deletion of YIH1 has no detectable effect on Gcn2 activity, suggesting that Yih1 is not a general inhibitor of
Externí odkaz:
https://doaj.org/article/0596328631ed47d090dc88db2982f095
Autor:
Cristian Cortez, Rafael M Martins, Renan M Alves, Richard C Silva, Luciana C Bilches, Silene Macedo, Vanessa D Atayde, Silvia Y Kawashita, Marcelo R S Briones, Nobuko Yoshida
Publikováno v:
PLoS Neglected Tropical Diseases, Vol 6, Iss 10, p e1804 (2012)
BackgroundDiversity of T. cruzi strains is a central problem in Chagas disease research because of its correlation with the wide range of clinical manifestations and the biogeographical parasite distribution. The role played by parasite microdiversit
Externí odkaz:
https://doaj.org/article/1007e355be034cc8abd44cb4df6e270f
Autor:
Su Jung, Lee, Rashmi, Ramesh, Valerie, de Boor, Jan M, Gebler, Richard C, Silva, Evelyn, Sattlegger
Publikováno v:
Yeast (Chichester, England). 34(9)
The common method for liberating proteins from Saccharomyces cerevisiae cells involves mechanical cell disruption using glass beads and buffer containing inhibitors (protease, phosphatase and/or kinase inhibitors), followed by centrifugation to remov
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3183cf3a555111333e5ee84cfbec7aa6
https://pubmed.ncbi.nlm.nih.gov/28568773
https://pubmed.ncbi.nlm.nih.gov/28568773
Publikováno v:
MethodsX, Vol 5, Iss , Pp 75-82 (2018)
Many studies require the detection and relative quantitation of proteins from cell culture samples using immunoblotting. Limiting factors are the cost of protease inhibitors, the time required to break cells and generate samples, as well as the high
Externí odkaz:
https://doaj.org/article/8398f1f465b24a48a75d6666cb1f13e2
Autor:
Zain Akram, Ishtiaq Ahmed, Heike Mack, Ramandeep Kaur, Richard C. Silva, Beatriz A. Castilho, Sylvie Friant, Evelyn Sattlegger, Alan L. Munn
Publikováno v:
Cells, Vol 9, Iss 3, p 672 (2020)
The budding yeast Saccharomyces cerevisiae has an actin cytoskeleton that comprises a set of protein components analogous to those found in the actin cytoskeletons of higher eukaryotes. Furthermore, the actin cytoskeletons of S. cerevisiae and of hig
Externí odkaz:
https://doaj.org/article/53130aeed9e94c908a481fdaa6eb383f