Zobrazeno 1 - 10
of 132
pro vyhledávání: '"Richard Brimacombe"'
Autor:
Dmitry V. Lesnyak, Alexey A. Bogdanov, Dmitry E. Burakovsky, Sergey V. Kiparisov, Petr V. Sergiev, Olga A. Dontsova, Andrei A. Leonov, Richard Brimacombe
Publikováno v:
Journal of Biological Chemistry. 280:31882-31889
Translocation catalyzed by elongation factor G occurs after the peptidyltransferase reaction on the large ribosomal subunit. Deacylated tRNA in the P-site stimulates multiple turnover GTPase activity of EF-G. We suggest that the allosteric signal fro
Publikováno v:
Journal of Biological Chemistry. 278:25664-25670
A method for preparation of Escherichia coli ribosomes carrying lethal mutations in 23 S rRNA was developed. The method is based on the site-directed incorporation of a streptavidin binding tag into functionally neutral sites of the 23 S rRNA and sub
Autor:
Richard Brimacombe, Jutta Rinke-Appel, Monika Osswald, Petr V. Sergiev, Klaus von Knoblauch, Olga A. Dontsova, Olga N. Avdeeva, Florian Mueller, Alexey A. Bogdanov
Publikováno v:
RNA. 8:612-625
Radioactively labeled 4.5S RNA containing statistically distributed 4-thiouridine residues in place of normal uridine was prepared by T7 transcription. The ability of this modified 4.5S RNA to form a complex with the protein Ffh was demonstrated by a
Autor:
Richard Brimacombe, Jutta Rinke-Appel, Barbara Greuer, Ingolf Sommer, Florian Mueller, Monika Osswald
Publikováno v:
The Ribosome
The structure of the bacterial ribosome, in particular that of Escherichia coli, has been studied over a period of several decades with the help of a wide variety of biochemical and biophysical techniques. This chapter summarizes briefly how the 70S
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4885cb6f96e181157c4e3bcab5f5ec4d
https://doi.org/10.1128/9781555818142.ch14
https://doi.org/10.1128/9781555818142.ch14
Autor:
Florian Mueller, Michael Huber, Paola Londei, Isabella Moll, Udo Bläsi, Richard Brimacombe, Sonja Grill, Pooneh Sairafi
Publikováno v:
Journal of Bacteriology. 183:3499-3505
Based on the complementarity of the initial coding region (downstream box [db]) of several bacterial and phage mRNAs to bases 1469 to 1483 in helix 44 of 16S rRNA (anti-downstream box [adb]), it has been proposed that db-adb base pairing enhances tra
Autor:
Richard Brimacombe, E van der Sluis, Andrei A. Leonov, K. von Knoblauch, Alexey A. Bogdanov, Tillmann Pape, Olga A. Dontsova, Florian Mueller, Monika Osswald, M. van Heel, Rishi Matadeen, Petr V. Sergiev
Publikováno v:
Journal of Molecular Biology. 307:1341-1349
Insertions were introduced by a two-step mutagenesis procedure into each of five double-helical regions of Escherichia coli 23 S rRNA, so as to extend the helix concerned by 17 bp. The helices chosen were at sites within the 23 S molecule (h9, h25, h
Autor:
Ingolf Sommer, Richard Brimacombe
Publikováno v:
Journal of Computational Chemistry. 22:407-417
A computational multiscale modeling approach that was used for the refinement of the structure of the bacterial ribosome is presented. Though designed for the ribosome, the methods are applicable to other large biomolecules as well. Algorithms were d
Autor:
Monika Osswald, Richard Brimacombe
Publikováno v:
Nucleic Acids Research. 27:2283-2290
Three contiguous fragments of Escherichia coli 5S rRNA were prepared by T7 transcription from synthetic DNA templates. The central fragment, comprising residues 33-71 of the molecule, was transcribed in the presence of 4-thiouridine triphosphate toge
Autor:
Olga A. Dontsova, A. N. Topin, Petr V. Sergiev, Svetlana Dokudovskaya, Richard Brimacombe, Elena A. Romanova, Alexey A. Bogdanov
Publikováno v:
Nucleic Acids Research. 26:2519-2525
Two photoreactive diazirine derivatives of uridine were used to study contacts between 5S rRNA and 23 rRNA in situ in Escherichia coli ribosomes. 2'-Amino-2'-deoxy-uridine or 5-methyleneaminouridine were introduced into 5S rRNA by T7 transcription. A
New features of 23S ribosomal RNA folding: The long helix 41–42 makes a 'U-turn' inside the ribosome
Publikováno v:
RNA. 4:658-668
23S rRNA from Escherichia coli was cleaved at single internucleotide bonds using ribonuclease H in the presence of appropriate chimeric oligonucleotides; the individual cleavage sites were between residues 384 and 385, 867 and 868, 1045 and 1046, and