Zobrazeno 1 - 10 of 13 pro vyhledávání: '"Richard B. Gayle"'
1
Site-Directed Mutagenesis of Human Endothelial Cell Ecto-ADPase/Soluble CD39: Requirement of Glutamate 174 and Serine 218 for Enzyme Activity and Inhibition of Platelet Recruitment
Autor: Charles R. Maliszewski, M. Johan Broekman, Aaron J. Marcus, Richard B. Gayle, Naziba Islam, Joan H.F. Drosopoulos
Publikováno v: Biochemistry. 39:6936-6943
Endothelial cell CD39/ecto-ADPase plays a major role in vascular homeostasis. It rapidly metabolizes ADP released from stimulated platelets, thereby preventing further platelet activation and recruitment. We recently developed a recombinant, soluble
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::470ca8896ea1c84c116b32141180020c
https://doi.org/10.1021/bi992581e
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2
The endothelial cell ecto-ADPase responsible for inhibition of platelet function is CD39
Autor: Richard B. Gayle, T. N. Alyonycheva, Aaron J. Marcus, M. A. Schoenborn, Charles R. Maliszewski, Joan H.F. Drosopoulos, M J Broekman, K. A. Schooley, L. B. Safier, Katherine A. Hajjar, David N. Posnett, Naziba Islam
Publikováno v: Journal of Clinical Investigation. 99:1351-1360
We previously demonstrated that when platelets are in motion and in proximity to endothelial cells, they become unresponsive to agonists (Marcus, A.J., L.B. Safier, K.A. Hajjar, H.L. Ullman, N. Islam, M.J. Broekman, and A.M. Eiroa. 1991. J. Clin. Inv
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::67d2bc4023013fb0fcf02faac654470d
https://doi.org/10.1172/jci119294
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3
Induction of recombinant gene expression in Escherichia coli using an alkaline pH shift
Autor: Kurt Poindexter, Richard B. Gayle
Publikováno v: Gene. 97:125-130
A commonly used approach to control recombinant protein production in Escherichia coli utilizes the lambda pL promoter-operator and the lambda repressor. Inactivation of the lambda repressor function allows transcription to proceed. However, inductio
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::084222b0df1ad6888a38c4e26299507e
https://doi.org/10.1016/0378-1119(91)90019-8
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4
A preliminary and comparative evaluation of a novel Ad5 [E1-, E2b-] recombinant-based vaccine used to induce cell mediated immune responses
Autor: Frank R. Jones, Joseph P. Balint, Lois H. Yoshida, Younong Xu, Andrea Amalfitano, Richard B. Gayle, Elizabeth Gabitzsch
Publikováno v: Immunology letters. 122(1)
Adenovirus vectors have been shown to be highly effective as vaccine platforms capable of inducing both humoral and cell mediated immune (CMI) responses. An Ad serotype 5 vector containing unique deletions in the E2b region (Ad5 [E1-, E2b-]) has been
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ecfff55dff6a4db56a87c3060d0f5199
https://pubmed.ncbi.nlm.nih.gov/19073216
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5
Role of the Cro repressor carboxy terminal domain and flexible dimer linkage in operator and nonspecific DNA binding
Autor: Laurent Bracco, Marvin H. Caruthers, Scott J. Eisenbeis, Richard B. Gayle, Graham Beaton, Adrian J. Hubbard
Publikováno v: Biochemistry. 29:9241-9249
A series of mutations comprising single and multiple substitutions, deletions, and extensions within the carboxy-terminal domain of the bacteriophage lambda Cro repressor have been constructed. These mutations generally affect the affinity of repress
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a65eb619f2474bbab2b29ca6e31c69a9
https://doi.org/10.1021/bi00491a019
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8
Thromboregulation by endothelial cells: significance for occlusive vascular diseases
Autor: Joan H.F. Drosopoulos, Aaron J. Marcus, M. Johan Broekman, Richard B. Gayle, Naziba Islam, David J. Pinsky, Charles R. Maliszewski
Publikováno v: Arteriosclerosis, thrombosis, and vascular biology. 21(2)
Abstract —During their 7- to 9-day lifespan in the circulation, platelets perform an ill-defined baseline function that maintains the integrity of the vasculature. In thrombocytopenic states, there is an increase in vascular permeability and fragil
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d5675103c487f96a25ef909d4c33f498
https://pubmed.ncbi.nlm.nih.gov/11156849
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9
Construction of interleukin-1 alpha mutants using unequal contamination of synthetic oligonucleotides
Autor: Kurt Poindexter, Richard B. Gayle, Rita Jerzy
Publikováno v: Nucleic acids research. 19(8)
Proteins without readily available three-dimensional structural data present a difficult problem in the exploration of structure/function relationships. Saturation mutagenesis using contaminated oligonucleotides can identify potentially interesting r
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a9760ec8ab809081da6eca8af99ca820
https://pubmed.ncbi.nlm.nih.gov/1840657
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