Zobrazeno 1 - 10
of 24
pro vyhledávání: '"Richard A. Nunamaker"'
Publikováno v:
Journal of Medical Entomology. 38:55-58
The successful long-term storage of insects that are used for research purposes can eliminate the need for ongoing colony maintenance on a large scale. In addition, rare and valuable genotypes of insects can be preserved. This study was conducted to
Autor:
Mona A. Abdallah, Richard A. Nunamaker, Keith E. Murphy, Richard S. Pollenz, Frans N. Droog, Walter J. Tabachnick
Publikováno v:
Biochemical Genetics. 38:377-390
Culicoides variipennis sonorensis is the primary vector of bluetongue viruses in North America. Glutathione S-transferases (GSTs) are enzymes that catalyze nucleophilic substitutions, converting reactive lipophilic molecules into soluble conjugates.
Publikováno v:
Biochemical Genetics. 38:154-162
A cDNA expression library constructed from Culicoides variipennis sonorensis was screened using an antibody specific for Hsp60 of Heliothis virescens. A single clone encoding the complete heat shock protein (Hsp60) of C. variipennis was identified an
Publikováno v:
Journal of Medical Entomology. 34:24-28
An avidin-biotin complex (ABC) dot-blot, an antigen capture enzyme-linked immunosorbent assay (ELISA), and immunoelectron microscopy (IEM) were used to detect bluetongue (BLU) virus and viral antigen in field-collected C. varriipennis sonorensis Wirt
Publikováno v:
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology. 113:73-77
In vivo protein expression in the abdominal viscera of C. v. sonorensis was examined from adult flies that were cold shocked for various lengths of time at 0, -10, or -15°C and labelled at 25°C with 35 S-methionine at 0, 2, 4 and 6 hr during the re
Autor:
Richard A. Nunamaker
Publikováno v:
Journal of Medical Entomology. 30:913-917
Rapid cold-hardening was studied in the adult stage of the biting midge Culicoides variipennis sonorensis WirthJones from a laboratory colony. No individuals could withstand a direct transfer from 20 degrees C (rearing temperature) to -10 degrees C f
Publikováno v:
Journal of Medical Entomology. 37:784-786
Vesicular stomatitis virus serotype New Jersey (VSNJV) was mixed with bovine blood or fetal bovine serum (FBS) and fed across silicone membranes to laboratory populations of Culicoides sonorensis Wirth & Jones. In an initial study, virus was detected
Publikováno v:
Journal of Medical Entomology. 28:74-85
When Culicoides variipennis (Coquillett) ingested a bluetongue virus (BTV)-defibrinated sheep blood suspension, BTV adsorbed to sheep red blood cells (RBCs) within 2 h. The virus had entered RBCs by 6 h and was still seen in RBCs 2 d after ingestion
Autor:
Peggy J Sieburth, Richard A. Nunamaker, James G. Wigington, Carol E Nunamaker, James O. Mecham, Victoria C. Dean
Publikováno v:
Comparative Biochemistry and Physiology Part A: Physiology. 96:19-31
1. 1. Culicoides variipennis midges were fed on a blood meal containing bluetongue virus (BTV) serotype 11 (BTV-11) and on four subsequent non-infective blood meals at 4-day intervals. 2. 2. Eggs were collected before each blood-feeding and reared to
Publikováno v:
American Entomologist. 36:18-27
NATURALLY PRESERVED ANIMALS and plants are of tremendous scientific value. Specimens preserved in ice, amber, or tar and fossilized by various means allow study of organisms and processes that no longer exist. Historically, a great deal of work has f