Zobrazeno 1 - 10
of 14
pro vyhledávání: '"Richard A. Fekete"'
Publikováno v:
BioTechniques, Vol 35, Iss 1, Pp 90-98 (2003)
Identification of nucleotides used for RNA chain initiation or for contacting DNA binding proteins is basic to our understanding of gene regulation. Normally, a radioactive primer is used to copy RNA or DNA. The polymerase extension stops at free end
Externí odkaz:
https://doaj.org/article/e8849ade0af245fe868c88920b1d9667
Publikováno v:
Molecular Microbiology. 62:1739-1751
In bacteria, many DNA-protein interactions that initiate transcription, replication and recombination require the mediation of DNA architectural proteins such as IHF and HU. For replication initiation, plasmid P1 requires three origin binding protein
Publikováno v:
Journal of Bacteriology. 188:1060-1070
Genome duplication and segregation normally are completed before cell division in all organisms. The temporal relation of duplication and segregation, however, can vary in bacteria. Chromosomal regions can segregate towards opposite poles as they are
Autor:
Sudeshna Kar, Richard A. Fekete, Sankar Adhya, Kira K. Lueders, Dean H. Hamer, Kenneth R. Henry, Louise McHugh, Stella Hu, Srinivas S. Rao, Qi Zhao
Publikováno v:
Proceedings of the National Academy of Sciences. 102:11993-11998
Most HIV transmission occurs on the mucosal surfaces of the gastrointestinal and cervicovaginal tracts, both of which are normally coated by a biofilm of nonpathogenic commensal bacteria. We propose to genetically engineer such naturally occurring ba
Autor:
Peter P. Papp, Richard A. Fekete, Ashish N. Basuray, Johan Paulsson, Majda Valjavec-Gratian, Dhruba K. Chattoraj, Nilangshu Das
Publikováno v:
Proceedings of the National Academy of Sciences. 102:2856-2861
Many organisms control initiation of DNA replication by limiting supply or activity of initiator proteins. In plasmids, such as P1, initiators are limited primarily by transcription and dimerization. However, the relevance of initiator limitation to
Autor:
Richard A. Fekete, Dhruba K. Chattoraj
Publikováno v:
Molecular Microbiology. 55:175-183
Eukaryotic chromosomes contain a locus, the centromere, at which force is applied to separate replicated chromosomes. A centromere analogue is also found in some bacterial plasmids and chromosomes, although not yet identified in the well-studied Esch
Publikováno v:
BioTechniques. 35:90-98
Identification of nucleotides used for RNA chain initiation or for contacting DNA binding proteins is basic to our understanding of gene regulation. Normally, a radioactive primer is used to copy RNA or DNA. The polymerase extension stops at free end
Autor:
Laura S. Frost, Richard A. Fekete
Publikováno v:
Journal of Biological Chemistry. 277:16705-16711
TraM is a DNA binding protein required for conjugative transfer of the self-transmissible IncF group of plasmids, including F, R1, and R100. F TraM binds to three sites in F oriT: two high affinity binding sites, sbmA and sbmB, which are direct repea
Autor:
Ronald V, Abruzzese, Richard A, Fekete
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 997
Analyzing gene expression profiles from cells en masse provides an average profile for the population which may obscure differences in individual cells. Using an optimized workflow for qRT-PCR, gene expression profiles of undifferentiated pluripotent
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 632
TaqMan Array Cards are high-throughput, accurate, sensitive, and simple-to-use tools for quantitative analysis of mRNA or miRNA transcripts using a real-time PCR protocol. They utilize a microfluidic card with 384 reaction chambers and eight sample l