Zobrazeno 1 - 10
of 63
pro vyhledávání: '"Richard A Pfuetzner"'
Publikováno v:
eLife, Vol 7 (2018)
The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (N-ethylmaleimide sensitive factor) and an adaptor protein, SNAP (soluble NSF atta
Externí odkaz:
https://doaj.org/article/16de592c06514366be70c0596def7c4f
Autor:
Ucheor B Choi, Minglei Zhao, K Ian White, Richard A Pfuetzner, Luis Esquivies, Qiangjun Zhou, Axel T Brunger
Publikováno v:
eLife, Vol 7 (2018)
SNARE complex disassembly by the ATPase NSF is essential for neurotransmitter release and other membrane trafficking processes. We developed a single-molecule FRET assay to monitor repeated rounds of NSF-mediated disassembly and reassembly of individ
Externí odkaz:
https://doaj.org/article/b6ef30140f2c4082a52d6af39cbdac0b
Autor:
Ying Lai, Jiajie Diao, Daniel J Cipriano, Yunxiang Zhang, Richard A Pfuetzner, Mark S Padolina, Axel T Brunger
Publikováno v:
eLife, Vol 3 (2014)
Previously we showed that fast Ca2+-triggered vesicle fusion with reconstituted neuronal SNAREs and synaptotagmin-1 begins from an initial hemifusion-free membrane point contact, rather than a hemifusion diaphragm, using a single vesicle–vesicle li
Externí odkaz:
https://doaj.org/article/8f0e2952d6a54f16a7698ee6aae9f16e
Autor:
Jeremy Leitz, Chuchu Wang, Luis Esquivies, Richard A. Pfuetzner, John Jacob Peters, Sergio Couoh-Cardel, Austin L. Wang, Axel T. Brunger
Publikováno v:
Cell Reports, Vol 43, Iss 5, Pp 114026- (2024)
Summary: Synaptic vesicle docking and priming are dynamic processes. At the molecular level, SNAREs (soluble NSF attachment protein receptors), synaptotagmins, and other factors are critical for Ca2+-triggered vesicle exocytosis, while disassembly fa
Externí odkaz:
https://doaj.org/article/e1056b181dfa43a28fe7d62ec5082454
Autor:
Julien R C Bergeron, Liam J Worrall, Nikolaos G Sgourakis, Frank DiMaio, Richard A Pfuetzner, Heather B Felise, Marija Vuckovic, Angel C Yu, Samuel I Miller, David Baker, Natalie C J Strynadka
Publikováno v:
PLoS Pathogens, Vol 9, Iss 4, p e1003307 (2013)
The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a "basal body", a lock-nut structure spanning bo
Externí odkaz:
https://doaj.org/article/4db4112da9e849c689b527d13a3699fa
Autor:
Nisha Gopal, Jeremy Leitz, Chuchu Wang, Luis Esquivies, Richard A. Pfuetzner, Axel T. Brunger
Publikováno v:
Current Research in Physiology, Vol 7, Iss , Pp 100121- (2024)
Synaptic vesicles specific to inhibitory GABA-releasing neurons are critical for regulating neuronal excitability. To study the specific molecular composition, architecture, and function of inhibitory synaptic vesicles, we have developed a new method
Externí odkaz:
https://doaj.org/article/676dd94ef0024c84ac2e9b4e8b0a1e30
Autor:
Klaudia Jaczynska, Luis Esquivies, Richard A. Pfuetzner, Baris Alten, Kyle D. Brewer, Qiangjun Zhou, Ege T. Kavalali, Axel T. Brunger, Josep Rizo
Publikováno v:
FEBS Open Bio, Vol 13, Iss 1, Pp 26-50 (2023)
Characterizing interactions of Synaptotagmin‐1 with the SNARE complex is crucial to understand the mechanism of neurotransmitter release. X‐ray crystallography revealed how the Synaptotagmin‐1 C2B domain binds to the SNARE complex through a so
Externí odkaz:
https://doaj.org/article/4c5a346bf0504f2d810b8e365e02f59e
Autor:
Yan Gao, Yousuf A. Khan, Weike Mo, K. Ian White, Matthew Perkins, Richard A. Pfuetzner, Josef G. Trapani, Axel T. Brunger, Teresa Nicolson
Publikováno v:
Cell Reports, Vol 42, Iss 4, Pp 112345- (2023)
Summary: The AAA+ NSF complex is responsible for SNARE complex disassembly both before and after membrane fusion. Loss of NSF function results in pronounced developmental and degenerative defects. In a genetic screen for sensory deficits in zebrafish
Externí odkaz:
https://doaj.org/article/fa469058d265459a95ab589acda03f97
Autor:
Kailu Yang, Chuchu Wang, Alex J. B. Kreutzberger, K. Ian White, Richard A. Pfuetzner, Luis Esquivies, Tomas Kirchhausen, Axel T. Brunger
Publikováno v:
Proceedings of the National Academy of Sciences. 120
The Omicron variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) introduced a relatively large number of mutations, including three mutations in the highly conserved heptad repeat 1 (HR1) region of the spike glycoprotein (S) critic
Autor:
Klaudia Jaczynska, Luis Esquivies, Richard A. Pfuetzner, Baris Alten, Kyle D. Brewer, Qiangjun Zhou, Ege T. Kavalali, Axel T. Brunger, Josep Rizo
Publikováno v:
FEBS open bioReferences.
Characterizing interactions of Synaptotagmin-1 with the SNARE complex is crucial to understand the mechanism of neurotransmitter release. X-ray crystallography revealed how the Synaptotagmin-1 C