Zobrazeno 1 - 10
of 22
pro vyhledávání: '"Richard A J, Darby"'
Autor:
Richard A. J. Darby, Stephane R. Gross, Roslyn M. Bill, Debasmita Sarkar, Mark P. Ashe, Stephanie P. Cartwright, Nicklas Bonander
Publikováno v:
Cartwright, S P, Darby, R A J, Sarkar, D, Bonander, N, Gross, S R, Ashe, M P & Bill, R M 2017, ' Constitutively-stressed yeast strains are high-yielding for recombinant Fps1 : Implications for the translational regulation of an aquaporin ', Microbial Cell Factories, vol. 16, no. 1, 41 . https://doi.org/10.1186/s12934-017-0656-2
Microbial Cell Factories
Microbial Cell Factories
Background We previously selected four strains of Saccharomyces cerevisiae for their ability to produce the aquaporin Fps1 in sufficient yield for further study. Yields from the yeast strains spt3Δ, srb5Δ, gcn5Δ and yTHCBMS1 (supplemented with 0.5
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f3177c23937555f49c52850cf7ecf37
https://doi.org/10.1186/s12934-017-0656-2
https://doi.org/10.1186/s12934-017-0656-2
Autor:
Chris Barton, Richard A. J. Darby, Matthieu Sollogoub, Keith R. Fox, Lynda J. Brown, Tom Brown, Nick Brown, Antonina Risitano, Catherine M. McKeen
Publikováno v:
Scopus-Elsevier
We have used oligonucleotides containing molecular beacons to determine melting profiles for intramolecular DNA duplexes, triplexes and quadruplexes (tetraplexes). The synthetic oligonucleotides used in these studies contain a fluorophore (fluorescei
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6dd4ebb3f18961598b2f64844764bcf0
https://doi.org/10.1093/nar/30.9.e39
https://doi.org/10.1093/nar/30.9.e39
Publikováno v:
ResearcherID
The multidrug resistant phenotype of cancer cells can often result from the over-production of a number of ATP binding cassette (ABC) transporters, including P-glycoprotein (P-gp). These multidrug efflux transporters expel administered anti-cancer dr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ac08f4f5cd380b2d0b2db449a2a417a4
https://doi.org/10.2174/138920011798357006
https://doi.org/10.2174/138920011798357006
Publikováno v:
Biotechnology and Bioengineering. 98:1103-1108
We have shown previously that a sequence-specific DNA-binding protein based on the Lac repressor protein can isolate pre-purified DNA efficiently from simple buffer solution but our attempts to purify plasmids directly from crude starting materials w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::916d9439c8e3daef8766d61de0809381
https://doi.org/10.1002/bit.21492
https://doi.org/10.1002/bit.21492
Autor:
Anna V. Hine, Anthony Gordon Crewe Hitchcock, Richard A. J. Darby, Gareth M. Forde, Siddhartha Ghose, Nigel K. H. Slater
Publikováno v:
Biotechnology and Bioengineering. 95:67-75
Current approaches for purifying plasmids from bacterial production systems exploit the physiochemical properties of nucleic acids in non-specific capture systems. In this study, an affinity system for plasmid DNA (pDNA) purification has been develop
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a40ebe2f8ddf98e44e982622ec1ac1cd
https://doi.org/10.1002/bit.20955
https://doi.org/10.1002/bit.20955
Autor:
Richard A. J. Darby, Anna V. Hine
Publikováno v:
The FASEB Journal. 19:1-20
Affinity purification of plasmid DNA is an attractive option for the biomanufacture of therapeutic plasmids, which are strictly controlled for levels of host protein, DNA, RNA, and endotoxin. Plasmid vectors are considered to be a safer alternative t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fd0809c921e27933c9652c47f330255e
https://doi.org/10.1096/fj.04-2812fje
https://doi.org/10.1096/fj.04-2812fje
Autor:
Mohammed, Jamshad, Richard A J, Darby
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 866
There are several ways to introduce non-native DNA into yeast cells, including chemical transformation and electroporation. Methods for both of these procedures are outlined in this chapter. Both methods permit the uptake of DNA from the environment
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::cdd0e9051a84c98e0501591c0ad4010b
https://pubmed.ncbi.nlm.nih.gov/22454114
https://pubmed.ncbi.nlm.nih.gov/22454114
Autor:
Mohammed, Jamshad, Richard A J, Darby
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 866
Yeast is a proven host for the production of recombinant proteins, which may be incorporated in cellular membranes or localized in subcellular compartments. In order to gain access to these proteins, cellular disruption is required to permit extracti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::987d47f676d72fdaaac89a7c8d6202f8
https://pubmed.ncbi.nlm.nih.gov/22454128
https://pubmed.ncbi.nlm.nih.gov/22454128
Autor:
Zharain, Bawa, Richard A J, Darby
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 866
A common method for inducing the production of recombinant proteins in Pichia pastoris is through the use of methanol. However, the by-products of methanol metabolism are toxic to yeast cells and therefore its addition to recombinant cultures must be
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::6b045ec31f80f22c7ea9d036b5863506
https://pubmed.ncbi.nlm.nih.gov/22454123
https://pubmed.ncbi.nlm.nih.gov/22454123
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 866
Having decided on yeast as a production host, the choice of species is often the first question any researcher new to the field will ask. With over 500 known species of yeast to date, this could pose a significant challenge. However, in reality, only
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e368414bbcd881921e7199934f64fc17
https://pubmed.ncbi.nlm.nih.gov/22454110
https://pubmed.ncbi.nlm.nih.gov/22454110