Zobrazeno 1 - 10
of 633
pro vyhledávání: '"Ribosomal depletion"'
Autor:
Zachary T. Herbert, Jamie P. Kershner, Vincent L. Butty, Jyothi Thimmapuram, Sulbha Choudhari, Yuriy O. Alekseyev, Jun Fan, Jessica W. Podnar, Edward Wilcox, Jenny Gipson, Allison Gillaspy, Kristen Jepsen, Sandra Splinter BonDurant, Krystalynne Morris, Maura Berkeley, Ashley LeClerc, Stephen D. Simpson, Gary Sommerville, Leslie Grimmett, Marie Adams, Stuart S. Levine
Publikováno v:
BMC Genomics, Vol 19, Iss 1, Pp 1-10 (2018)
Abstract Background Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of ma
Externí odkaz:
https://doaj.org/article/5f84f85b08874e36850d23efaeb4633c
Akademický článek
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Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
Lymphatic filariasis is caused by parasitic nematodes and is a leading cause of disability worldwide. Many filarial worms contain the bacterium Wolbachia as an obligate endosymbiont. RNA sequencing is a common technique used to study their molecular
Externí odkaz:
https://doaj.org/article/f0e5a1b5bec54b91aa22c18ee44ba2e9
Autor:
Stephen Simpson, Maura Berkeley, Allison F. Gillaspy, Edward Wilcox, Jamie P. Kershner, Gary Sommerville, Stuart S. Levine, Leslie Grimmett, Jyothi Thimmapuram, Vincent L. Butty, Jessica W. Podnar, Krystalynne Morris, Sulbha Choudhari, Marie Adams, Yuriy O. Alekseyev, Zachary T. Herbert, Sandra Splinter BonDurant, Jun Fan, Kristen Jepsen, Ashley LeClerc, Jenny Gipson
Publikováno v:
BMC Genomics, Vol 19, Iss 1, Pp 1-10 (2018)
BMC Genomics
BioMed Central
BMC Genomics
BioMed Central
Background Ribosomal RNA (rRNA) comprises at least 90% of total RNA extracted from mammalian tissue or cell line samples. Informative transcriptional profiling using massively parallel sequencing technologies requires either enrichment of mature poly
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4d619c86a18152de2cb83e56952101d3
https://doi.org/10.1186/s12864-018-4585-1
https://doi.org/10.1186/s12864-018-4585-1
Autor:
Herbert, Zachary, Kershner, Jamie, Butty, Vincent, Jyothi Thimmapuram, Sulbha Choudhari, Alekseyev, Yuriy, Fan, Jun, Podnar, Jessica, Wilcox, Edward, Gipson, Jenny, Gillaspy, Allison, Jepsen, Kristen, BonDurant, Sandra, Krystalynne Morris, Berkeley, Maura, LeClerc, Ashley, Simpson, Stephen, Sommerville, Gary, Grimmett, Leslie, Adams, Marie, Levine, Stuart
Figure S4. Fraction of reads mapping to each ERCC mRNA is shown for each replicate. Light horizontal lines show 2-fold changes in fraction observed (log scale). Each expected concentration is shown in a different color. Data sets ordered by intact/de
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::140306917b633e94d7089119471dc2ad
Autor:
Herbert, Zachary, Kershner, Jamie, Butty, Vincent, Jyothi Thimmapuram, Sulbha Choudhari, Alekseyev, Yuriy, Fan, Jun, Podnar, Jessica, Wilcox, Edward, Gipson, Jenny, Gillaspy, Allison, Jepsen, Kristen, BonDurant, Sandra, Krystalynne Morris, Berkeley, Maura, LeClerc, Ashley, Simpson, Stephen, Sommerville, Gary, Grimmett, Leslie, Adams, Marie, Levine, Stuart
Contains the following: Describes normalization of kit protocols and any individual site deviations from this normalization. Figure S1. – BioAnalyzer traces for samples used in the study. Left: Intact UHR, Right: Heat degraded UHR RNA. Figure S2.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1860a65c1b3f0dd72136773da944995c
Autor:
Herbert, Zachary, Kershner, Jamie, Butty, Vincent, Jyothi Thimmapuram, Sulbha Choudhari, Alekseyev, Yuriy, Fan, Jun, Podnar, Jessica, Wilcox, Edward, Gipson, Jenny, Gillaspy, Allison, Jepsen, Kristen, BonDurant, Sandra, Krystalynne Morris, Berkeley, Maura, LeClerc, Ashley, Simpson, Stephen, Sommerville, Gary, Grimmett, Leslie, Adams, Marie, Levine, Stuart
Figure S2. Clustering of differentially detected genes. Top 50 most differentially detected genes, as measured by variance of log2RPKM across all samples, were clustered based on their differential expression. TOP: Hierarchical tree of clustering bas
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad26cf714fcaf75f685183440ee33036
Autor:
Herbert, Zachary, Kershner, Jamie, Butty, Vincent, Jyothi Thimmapuram, Sulbha Choudhari, Alekseyev, Yuriy, Fan, Jun, Podnar, Jessica, Wilcox, Edward, Gipson, Jenny, Gillaspy, Allison, Jepsen, Kristen, BonDurant, Sandra, Krystalynne Morris, Berkeley, Maura, LeClerc, Ashley, Simpson, Stephen, Sommerville, Gary, Grimmett, Leslie, Adams, Marie, Levine, Stuart
Figure S3. Insert size distribution for RNAseq libraries from intact RNA. The insert size for each library passing the 50% rRNA filter was calculated for reads with convergent reads that were separated by
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1cbaa390d2e9ea3125f1b5a12218785c
Autor:
Herbert, Zachary, Kershner, Jamie, Butty, Vincent, Jyothi Thimmapuram, Sulbha Choudhari, Alekseyev, Yuriy, Fan, Jun, Podnar, Jessica, Wilcox, Edward, Gipson, Jenny, Gillaspy, Allison, Jepsen, Kristen, BonDurant, Sandra, Krystalynne Morris, Berkeley, Maura, LeClerc, Ashley, Simpson, Stephen, Sommerville, Gary, Grimmett, Leslie, Adams, Marie, Levine, Stuart
Figure S1. BioAnalyzer traces for samples used in the study. Left: Intact UHR, Right: Heat degraded UHR RNA. (PPTX 127Â kb)
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::175873692817b14c8af95b14e00fca4d
Publikováno v:
Frontiers in Microbiology; 2024, p1-14, 14p