Deuterium spin relaxation of fractionally deuterated ribonuclease H using paired 475 and 950 MHz NMR spectrometers.

Autor: Bhattacharya S; New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA., Varney KM; University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD, 21201, USA., Dahmane T; New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA., Johnson BA; Structural Biology Initiative, CUNY Advanced Science Research Center, 85 St. Nicholas Terrace, New York, NY, 10031, USA., Weber DJ; University of Maryland School of Medicine, 108 North Greene Street, Baltimore, MD, 21201, USA., Palmer AG 3rd; New York Structural Biology Center, 89 Convent Ave, New York, NY, 10027, USA. agp6@columbia.edu.; Department of Biochemistry and Molecular Biophysics, Columbia University, 630 West 168th Street, New York, NY, 10032, USA. agp6@columbia.edu.

Publikováno v: Journal of biomolecular NMR [J Biomol NMR] 2024 Sep; Vol. 78 (3), pp. 169-177. Date of Electronic Publication: 2024 Jun 10.

Selection pressures on evolution of ribonuclease H explored with rigorous free-energy-based design.

Autor: Hayes RL; Department of Chemical and Biomolecular Engineering, University of California, Irvine, CA 92697.; Department of Chemistry, University of Michigan, Ann Arbor, MI 48109., Nixon CF; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720., Marqusee S; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720.; California Institute for Quantitative Biosciences, University of California, Berkeley, CA 94720.; Department of Chemistry, University of California, Berkeley, CA 94720., Brooks CL 3rd; Department of Chemistry, University of Michigan, Ann Arbor, MI 48109.; Biophysics Program, University of Michigan, Ann Arbor, MI 48109.

Publikováno v: Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2024 Jan 16; Vol. 121 (3), pp. e2312029121. Date of Electronic Publication: 2024 Jan 09.

Origin and Evolution of Plant Long Terminal Repeat Retrotransposons with Additional Ribonuclease H.

Autor: Biryukov M; Sector of Molecular and Genetic Mechanisms of Regeneration, Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.; Novosibirsk State University, Novosibirsk, Russia., Ustyantsev K; Sector of Molecular and Genetic Mechanisms of Regeneration, Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.

Publikováno v: Genome biology and evolution [Genome Biol Evol] 2023 Sep 04; Vol. 15 (9).

Identification and assessment of the 1,6-dihydroxy-pyridin-2-one moiety as privileged scaffold for HBV ribonuclease H inhibition.

Autor: Giannakopoulou E; School of Health Sciences, Department of Pharmacy, Division of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece., Pardali V; School of Health Sciences, Department of Pharmacy, Division of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece., Edwards TC; Molecular Microbiology and Immunology, Saint Louis University School of Medicine, Saint Louis, MO, 63104, United States., Woodson M; Molecular Microbiology and Immunology, Saint Louis University School of Medicine, Saint Louis, MO, 63104, United States., Tajwar R; Molecular Microbiology and Immunology, Saint Louis University School of Medicine, Saint Louis, MO, 63104, United States., Tavis JE; Molecular Microbiology and Immunology, Saint Louis University School of Medicine, Saint Louis, MO, 63104, United States., Zoidis G; School of Health Sciences, Department of Pharmacy, Division of Pharmaceutical Chemistry, National and Kapodistrian University of Athens, Panepistimiopolis Zografou, 15771, Athens, Greece. Electronic address: zoidis@pharm.uoa.gr.

Publikováno v: Antiviral research [Antiviral Res] 2024 Mar; Vol. 223, pp. 105833. Date of Electronic Publication: 2024 Feb 05.

Discovery of Benzisothiazolone Derivatives as Bifunctional Inhibitors of HIV-1 Reverse Transcriptase DNA Polymerase and Ribonuclease H Activities

Autor: Alondra Vázquez Rivera, Heather Donald, Mounia Alaoui-El-Azher, John J. Skoko, John S. Lazo, Michael A. Parniak, Paul A. Johnston, Nicolas Sluis-Cremer

Publikováno v: Biomolecules, Vol 14, Iss 7, p 819 (2024)

The ribonuclease H (RNase H) active site of HIV-1 reverse transcriptase (RT) is the only viral enzyme not targeted by approved antiretroviral drugs. Using a fluorescence-based in vitro assay, we screened 65,239 compounds at a final concentration of 1

Externí odkaz: https://doaj.org/article/f8e64f106e54456d83df7089a79fcab8