Process for Assembly and Transformation into Saccharomyces cerevisiae of a Synthetic Yeast Artificial Chromosome Containing a Multigene Cassette to Express Enzymes That Enhance Xylose Utilization Designed for an Automated Platform.

Autor: Hughes SR; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, Peoria, IL, USA stephen.hughes@ars.usda.gov., Cox EJ; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, Peoria, IL, USA Department of Chemistry and Biochemistry, Bradley University, Peoria, IL, USA., Bang SS; South Dakota School of Mines & Technology, Chemical and Biological Engineering, Rapid City, SD, USA., Pinkelman RJ; South Dakota School of Mines & Technology, Chemical and Biological Engineering, Rapid City, SD, USA., López-Núñez JC; National Coffee Research Centre (Cenicafe), National Federation of Coffee Growers of Colombia (FNC), Manizales, Caldas, Colombia., Saha BC; USDA, ARS, NCAUR, Bioenergy Research Unit, Peoria, IL, USA., Qureshi N; USDA, ARS, NCAUR, Bioenergy Research Unit, Peoria, IL, USA., Gibbons WR; Department of Biology and Microbiology, South Dakota State University, Brookings, SD, USA., Fry MR; Department of Chemistry and Biochemistry, Bradley University, Peoria, IL, USA., Moser BR; USDA, ARS, NCAUR, Bio-Oils Research Unit, Peoria, IL, USA., Bischoff KM; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, Peoria, IL, USA., Liu S; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology Research Unit, Peoria, IL, USA., Sterner DE; Progenra, Inc., Malvern, PA, USA., Butt TR; LifeSensors, Inc., Malvern, PA, USA., Riedmuller SB; Hudson Control Group, Inc., Springfield, NJ, USA., Jones MA; Department of Chemistry, Illinois State University, Normal, IL, USA., Riaño-Herrera NM; National Coffee Research Centre (Cenicafe), National Federation of Coffee Growers of Colombia (FNC), Manizales, Caldas, Colombia.

Publikováno v: Journal of laboratory automation [J Lab Autom] 2015 Dec; Vol. 20 (6), pp. 621-35. Date of Electronic Publication: 2015 Feb 26.

Automated UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 and selection for microaerophilic growth and ethanol production at elevated temperature on biomass sugars.

Autor: Hughes SR; United States Department of Agriculture (USDA), Agricultural Research Service (ARS), National Center for Agricultural Utilization Research (NCAUR), Renewable Product Technology (RPT) Research Unit, Peoria, IL, USA. Stephen.Hughes@ars.usda.gov, Bang SS, Cox EJ, Schoepke A, Ochwat K, Pinkelman R, Nelson D, Qureshi N, Gibbons WR, Kurtzman CP, Bischoff KM, Liu S, Cote GL, Rich JO, Jones MA, Cedeño D, Doran-Peterson J, Riaño-Herrera NM, Rodríguez-Valencia N, López-Núñez JC

Publikováno v: Journal of laboratory automation [J Lab Autom] 2013 Aug; Vol. 18 (4), pp. 276-90. Date of Electronic Publication: 2013 Mar 29.