Detecting intracellular thiol redox state in leukaemia and heterogeneous immune cell populations: An optimised protocol for digital flow cytometers

Autor: Alex J. Wadley, Rhys G. Morgan, Kate J. Heesom, Paul S. Hole, Steven J. Coles

Publikováno v: MethodsX, Vol 5, Iss , Pp 1473-1483 (2018)

Flow cytometric methods for detecting and quantifying reduced intracellular thiol content using fluorescein-5-maleimide (F5M) in viable eukaryotic cells date back to 1983 (Durand and Olive [1]). There has been little development in these methodologie

Externí odkaz: https://doaj.org/article/705c6ca9808142cca36f0c7c6650d22f

The PDK1 master kinase is over-expressed in acute myeloid leukemia and promotes PKC-mediated survival of leukemic blasts

Autor: Joanna Zabkiewicz, Lorna Pearn, Robert K. Hills, Rhys G. Morgan, Alex Tonks, Alan K. Burnett, Richard L. Darley

Publikováno v: Haematologica, Vol 99, Iss 5 (2014)

PDK1 is a master kinase that activates at least six protein kinase groups including AKT, PKC and S6K and is a potential target in the treatment of a range of malignancies. Here we show overexpression of PDK1 in over 40% of myelomonocytic acute leukem

Externí odkaz: https://doaj.org/article/4918f0a34d444548abd0899cbb4f7bb5

Targeting β-catenin in acute myeloid leukaemia: past, present, and future perspectives

Autor: Megan Wagstaff, Brandon Coke, Georgia R. Hodgkiss, Rhys G. Morgan

Acute myeloid leukaemia (AML) is an aggressive disease of the bone marrow with a poor prognosis. Evidence suggests long established chemotherapeutic regimens used to treat AML are reaching the limits of their efficacy, necessitating the urgent develo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a57e4404ecc9444219e65e71c060195b
http://sro.sussex.ac.uk/id/eprint/105241/1/BSR-2021-1841CR1_Merged_PDF.pdf

Crosstalk between β-catenin and WT1 signaling activity in acute myeloid leukemia

Autor: Allison Blair, Erika J. Mancini, O Tsaponina, Stefan G. E. Roberts, L Milton-Harris, Amanda Jayne Tonks, G Caalim, M Payne, Richard Lawrence Darley, Kate J. Heesom, H Greenfield, Rhys G. Morgan

Publikováno v: Haematologica. 108(1)

Wnt signalling is an evolutionary conserved signal transduction pathway heavily implicated in normal development and disease. The central mediator of this pathway, β-catenin, is frequently overexpressed, mislocalised and overactive in acute myeloid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c3583d5ab566ee7c5a7ccd4eaf1f4883
https://pubmed.ncbi.nlm.nih.gov/35443562

Inhibition of GATA2 restrains cell proliferation and enhances apoptosis and chemotherapy mediated apoptosis in human GATA2 overexpressing AML cells

Autor: Alex Gibbs, Samantha Sinnadurai, Leigh-anne Thomas, Zhengke Wang, Alfonso Garcia-Valverde, Neil P. Rodrigues, Rhys G. Morgan, Allison Blair, Maria Konstantinou, Ashleigh S. Boyd, Magali Boyer, Juan Bautista Menendez-Gonzalez

Publikováno v: Menendez-Gonzalez, J B, Sinnadurai, S, Gibbs, A, Thomas, L, Konstantinou, M, Garcia-Valverde, A, Boyer, M, Wang, Z, Boyd, A S, Blair, A, Morgan, R G & Rodrigues, N P 2019, ' Inhibition of GATA2 restrains cell proliferation and enhances apoptosis and chemotherapy mediated apoptosis in human GATA2 overexpressing AML cells ', Scientific Reports, vol. 9, 12212 (2019) . https://doi.org/10.1038/s41598-019-48589-0
Scientific Reports, Vol 9, Iss 1, Pp 1-8 (2019)
Scientific Reports

GATA2, a zinc finger transcription factor predominantly expressed in hematopoietic cells, acts as an essential regulator of hematopoietic stem cell generation, survival and functionality. Loss and gain of GATA2 expression has been implicated in myelo

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed752adc01c3daba6e99fc29f36f8c76
https://hdl.handle.net/1983/0fc9de71-230b-42b8-abd9-5194ca97349c