Zobrazeno 1 - 10
of 47
pro vyhledávání: '"Rex Lovrien"'
Publikováno v:
Journal of Agricultural and Food Chemistry. 50:6599-6604
Human red blood cell (RBC, erythrocyte) membranes have internal protein skeletons that govern the cells' distinctive discocyte-echinocyte morphology (shape) changes, seen in conventional microscopy. Glycophorin, the cell's transmembrane protein, pres
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc58e057afe0d99ead090284653f69aa
https://doi.org/10.1021/jf020261n
https://doi.org/10.1021/jf020261n
Publikováno v:
Separation Science and Technology. 35:1795-1811
Ligands are being developed for the upstream isolation-purification of sought-for proteins from dilute crudes by ligand–protein coprecipitation. The ligands are alkane-substituted azoaromatic anions (dyes) with sulfonate heads. Overall coprecipitat
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b91b54f8886b539a76dc8e3efd8fb111
https://doi.org/10.1081/ss-100102494
https://doi.org/10.1081/ss-100102494
Publikováno v:
Journal of Molecular Catalysis B: Enzymatic. 7:21-36
Selected azoaromatic sulfonate anions protect enzymes from inactivation by acid and elevated temperatures. These anionic sulfonate ligands bind to enzyme molecules by forming ion pairs between negatively charged sulfonate groups and positively charge
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::5d207f61df54aedc0183de9ea06cbeb0
https://doi.org/10.1016/s1381-1177(99)00019-3
https://doi.org/10.1016/s1381-1177(99)00019-3
Publikováno v:
Biopolymers. 49:451-458
1-Anilino-8-naphthalene sulfonate (ANS) anion is conventionally considered to bind to preexisting hydrophobic (nonpolar) surfaces of proteins, primarily through its nonpolar anilino-naphthalene group. Such binding is followed by an increase in ANS fl
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::3ea6ae038f627db735cb5acf0a568cda
https://doi.org/10.1002/(sici)1097-0282(199905)49:6<451::aid-bip3>3.0.co
https://doi.org/10.1002/(sici)1097-0282(199905)49:6<451::aid-bip3>3.0.co
Publikováno v:
Analytical Biochemistry. 257:33-39
Matrix ligands are intended for upstream use with dilute crudes on a large scale, splitting out soughtfor proteins by coprecipitating them as dense, protected aggregates. Matrix ligand coprecipitation is rapidly, quantitatively reversible, by pH shif
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::66971ab17bcce37b18302b35c9a8765b
https://doi.org/10.1006/abio.1997.2540
https://doi.org/10.1006/abio.1997.2540
Autor:
Clive Dennison, Rex Lovrien
Publikováno v:
Protein Expression and Purification. 11:149-161
Three phase partitioning (TPP) uses t-butanol and ammonium sulfate to precipitate enzymes and proteins from aqueous solutions. The method is useful both upstream with crude samples and downstream where a scaleable simple step is needed. About 25 enzy
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e14c3ece37bf89abe6821053aa663295
https://doi.org/10.1006/prep.1997.0779
https://doi.org/10.1006/prep.1997.0779
Publikováno v:
Journal of Molecular Recognition. 9:433-443
Matrix ligands are agents for isolating proteins out of dilute crudes by coprecipitating proteins. The ligands have a strong anion sulfonate head which initiates binding to proteins having a positive net charge, ZH+ approximately 5-20. Initial bindin
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f585ee70284d895d32592b17054d2be
https://doi.org/10.1002/(sici)1099-1352(199634/12)9:5/6<433::aid-jmr280>3.0.co
https://doi.org/10.1002/(sici)1099-1352(199634/12)9:5/6<433::aid-jmr280>3.0.co
Autor:
Rex Lovrien, Mark L. Ferrey
Publikováno v:
Talanta. 40:127-134
Adapted bacteria used in heat conduction calorimetry may be developed as ‘analytical reagents’ for compounds that can be metabolized by such bacteria. Adaption can be done by growing cells such as E. coli on the analyte of interest, for example a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6c19ca604381f8f4c02a37eab4ac108c
https://doi.org/10.1016/0039-9140(93)80311-e
https://doi.org/10.1016/0039-9140(93)80311-e
Autor:
Rex Lovrien, Mark J. Conroy
Publikováno v:
Journal of Crystal Growth. 122:213-222
Instead of trying to crystallize or precipitate amino acids and proteins as homogeneous products, often it is easier to coprecipitate or to cocrystallize them. Organic ionic ligands with large apolar groups bind to the solute or compound sought to is
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::1c98025eeb3ac03f1d8cf2f923bc6848
https://doi.org/10.1016/0022-0248(92)90248-h
https://doi.org/10.1016/0022-0248(92)90248-h
Publikováno v:
Thermochimica Acta. 172:187-195
When bacteria are adapted to metabolize organic compounds as carbon sources, in effect they become reactive reagents for analysis of specific compounds via calorimetry of their aerobic metabolic heat. From 2 to 5 mg. of adapted bacteria drive metabol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b2184a39e64b27b6d3b3728b1b3aca5f
https://doi.org/10.1016/0040-6031(90)80573-h
https://doi.org/10.1016/0040-6031(90)80573-h